Abstract
DNA repair and colony survival were measured in fibroblasts from a patient with Fanconi's anemia, HG 261, and from normal human donors after exposure to these cells to the cross-linking agent mitomycin C, X-rays or ultraviolet light. Survival was similar in HG 261 and normal cells after X-ray or ultraviolet radiation, but was reduced in the Fanconi's anemia cells after treatment with mitomycin C. The level of DNA cross-linking, as measured by the method of alkaline elution, was the same in both cell strains after exposure to various doses of mitomycin C. With incubation after drug treatment, a gradual decrease in the amount of cross-linking was observed, the rate of this apparent repair of cross-link damage was the same in both normal and HG 261 cells. The rejoining of DNA single strand breaks after X-irradiation and the production of excision breaks after ultraviolet radiation were also normal in HG 261 cells as determined by alkaline elution.
Fornace, Jr, A J;
Little, J B;
[1]
Weichselbaum, R R
[2]
- Harvard School of Public Health, Boston, MA (USA)
- Harvard Medical School, Boston, MA (USA)
Citation Formats
Fornace, Jr, A J, Little, J B, and Weichselbaum, R R.
DNA repair in a Fanconi's anemia fibroblast cell strain.
Netherlands: N. p.,
1979.
Web.
doi:10.1016/0005-2787(79)90494-5.
Fornace, Jr, A J, Little, J B, & Weichselbaum, R R.
DNA repair in a Fanconi's anemia fibroblast cell strain.
Netherlands.
https://doi.org/10.1016/0005-2787(79)90494-5
Fornace, Jr, A J, Little, J B, and Weichselbaum, R R.
1979.
"DNA repair in a Fanconi's anemia fibroblast cell strain."
Netherlands.
https://doi.org/10.1016/0005-2787(79)90494-5.
@misc{etde_5842965,
title = {DNA repair in a Fanconi's anemia fibroblast cell strain}
author = {Fornace, Jr, A J, Little, J B, and Weichselbaum, R R}
abstractNote = {DNA repair and colony survival were measured in fibroblasts from a patient with Fanconi's anemia, HG 261, and from normal human donors after exposure to these cells to the cross-linking agent mitomycin C, X-rays or ultraviolet light. Survival was similar in HG 261 and normal cells after X-ray or ultraviolet radiation, but was reduced in the Fanconi's anemia cells after treatment with mitomycin C. The level of DNA cross-linking, as measured by the method of alkaline elution, was the same in both cell strains after exposure to various doses of mitomycin C. With incubation after drug treatment, a gradual decrease in the amount of cross-linking was observed, the rate of this apparent repair of cross-link damage was the same in both normal and HG 261 cells. The rejoining of DNA single strand breaks after X-irradiation and the production of excision breaks after ultraviolet radiation were also normal in HG 261 cells as determined by alkaline elution.}
doi = {10.1016/0005-2787(79)90494-5}
journal = []
volume = {561:1}
journal type = {AC}
place = {Netherlands}
year = {1979}
month = {Jan}
}
title = {DNA repair in a Fanconi's anemia fibroblast cell strain}
author = {Fornace, Jr, A J, Little, J B, and Weichselbaum, R R}
abstractNote = {DNA repair and colony survival were measured in fibroblasts from a patient with Fanconi's anemia, HG 261, and from normal human donors after exposure to these cells to the cross-linking agent mitomycin C, X-rays or ultraviolet light. Survival was similar in HG 261 and normal cells after X-ray or ultraviolet radiation, but was reduced in the Fanconi's anemia cells after treatment with mitomycin C. The level of DNA cross-linking, as measured by the method of alkaline elution, was the same in both cell strains after exposure to various doses of mitomycin C. With incubation after drug treatment, a gradual decrease in the amount of cross-linking was observed, the rate of this apparent repair of cross-link damage was the same in both normal and HG 261 cells. The rejoining of DNA single strand breaks after X-irradiation and the production of excision breaks after ultraviolet radiation were also normal in HG 261 cells as determined by alkaline elution.}
doi = {10.1016/0005-2787(79)90494-5}
journal = []
volume = {561:1}
journal type = {AC}
place = {Netherlands}
year = {1979}
month = {Jan}
}