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DNA repair in a Fanconi's anemia fibroblast cell strain

Abstract

DNA repair and colony survival were measured in fibroblasts from a patient with Fanconi's anemia, HG 261, and from normal human donors after exposure to these cells to the cross-linking agent mitomycin C, X-rays or ultraviolet light. Survival was similar in HG 261 and normal cells after X-ray or ultraviolet radiation, but was reduced in the Fanconi's anemia cells after treatment with mitomycin C. The level of DNA cross-linking, as measured by the method of alkaline elution, was the same in both cell strains after exposure to various doses of mitomycin C. With incubation after drug treatment, a gradual decrease in the amount of cross-linking was observed, the rate of this apparent repair of cross-link damage was the same in both normal and HG 261 cells. The rejoining of DNA single strand breaks after X-irradiation and the production of excision breaks after ultraviolet radiation were also normal in HG 261 cells as determined by alkaline elution.
Authors:
Fornace, Jr, A J; Little, J B; [1]  Weichselbaum, R R [2] 
  1. Harvard School of Public Health, Boston, MA (USA)
  2. Harvard Medical School, Boston, MA (USA)
Publication Date:
Jan 26, 1979
Product Type:
Journal Article
Reference Number:
AIX-10-460966; EDB-80-013729
Resource Relation:
Journal Name: Biochim. Biophys. Acta - Nucl. Acids Protein Synth.; (Netherlands); Journal Volume: 561:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; ANEMIAS; COLONY FORMATION; CROSS-LINKING; FIBROBLASTS; MITOMYCIN; STRAND BREAKS; ULTRAVIOLET RADIATION; X RADIATION; ANIMAL CELLS; ANTIBIOTICS; ANTIMITOTIC DRUGS; BIOLOGICAL RECOVERY; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; HEMIC DISEASES; IONIZING RADIATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POLYMERIZATION; RADIATIONS; RECOVERY; REPAIR; SOMATIC CELLS; SYMPTOMS; 560400* - Other Environmental Pollutant Effects
OSTI ID:
5842965
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BBNPA
Submitting Site:
INIS
Size:
Pages: 99-109
Announcement Date:
Jul 01, 1979

Citation Formats

Fornace, Jr, A J, Little, J B, and Weichselbaum, R R. DNA repair in a Fanconi's anemia fibroblast cell strain. Netherlands: N. p., 1979. Web. doi:10.1016/0005-2787(79)90494-5.
Fornace, Jr, A J, Little, J B, & Weichselbaum, R R. DNA repair in a Fanconi's anemia fibroblast cell strain. Netherlands. doi:10.1016/0005-2787(79)90494-5.
Fornace, Jr, A J, Little, J B, and Weichselbaum, R R. 1979. "DNA repair in a Fanconi's anemia fibroblast cell strain." Netherlands. doi:10.1016/0005-2787(79)90494-5. https://www.osti.gov/servlets/purl/10.1016/0005-2787(79)90494-5.
@misc{etde_5842965,
title = {DNA repair in a Fanconi's anemia fibroblast cell strain}
author = {Fornace, Jr, A J, Little, J B, and Weichselbaum, R R}
abstractNote = {DNA repair and colony survival were measured in fibroblasts from a patient with Fanconi's anemia, HG 261, and from normal human donors after exposure to these cells to the cross-linking agent mitomycin C, X-rays or ultraviolet light. Survival was similar in HG 261 and normal cells after X-ray or ultraviolet radiation, but was reduced in the Fanconi's anemia cells after treatment with mitomycin C. The level of DNA cross-linking, as measured by the method of alkaline elution, was the same in both cell strains after exposure to various doses of mitomycin C. With incubation after drug treatment, a gradual decrease in the amount of cross-linking was observed, the rate of this apparent repair of cross-link damage was the same in both normal and HG 261 cells. The rejoining of DNA single strand breaks after X-irradiation and the production of excision breaks after ultraviolet radiation were also normal in HG 261 cells as determined by alkaline elution.}
doi = {10.1016/0005-2787(79)90494-5}
journal = {Biochim. Biophys. Acta - Nucl. Acids Protein Synth.; (Netherlands)}
volume = {561:1}
journal type = {AC}
place = {Netherlands}
year = {1979}
month = {Jan}
}