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First biological experiments at a vertical proton beam

Abstract

At the tandem accelerator laboratories in Munich and Erlangen vertical beamlines were installed last year. The advantage of a vertical beamline is that cells can be irradiated in a medium at 37 C and with simultaneous gassing, therefore also in physiological conditions. First experiments were carried out at the accelerator in Munich with a proton energy of 25 MeV. Chinese Hamster cells B14 were irradiated in Petri dishes where the base was of 1 mm polystyrol or 2 {mu}m hostaphan foils. The cell survival was measured by the cell survival assay and the repopulation of the colonies by the total colony volume. A solution of DNA with protein was irradiated to study DNA double strand breaks by constant field gel electrophoresis and DNA protein crosslinks by the nitrocellulose filter assay. For cell survival, total colony volume and DNA double-strand breaks X-rays and protons gave corresponding results, while with protons, higher yields of DNA-protein crosslinks were observed than with X-rays. (orig.)
Authors:
Distel, L; Distel, B; Roessner, B; Schwotzer, G; Sauer, R; [1]  Eyrich, W; Fritsch, M; Teufel, A; [2]  Besserer, J; Boer, J de; Moosburger, M; Quicken, P [3] 
  1. Erlangen-Nuernberg Univ., Erlangen (Germany). Klinik fuer Strahlentherapie
  2. Erlangen-Nuernberg Univ., Erlangen (Germany). Physikalisches Inst.
  3. Muenchen Univ. (Germany). Sektion Physik
Publication Date:
Sep 01, 1997
Product Type:
Conference
Report Number:
GSI-97-09; CONF-9709224-
Reference Number:
SCA: 560120; PA: DEN-98:0F5442; EDB-98:035773; SN: 98001927295
Resource Relation:
Conference: 6. workshop on heavy-charged particles in biology and medicine, Baveno (Italy), 29 Sep - 1 Oct 1997; Other Information: PBD: Sep 1997; Related Information: Is Part Of 6. Workshop on heavy-charged particles in biology and medicine. Book of abstracts; Kraft, G.; Langbein, K. [eds.]; PB: 290 p.
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; TANDEM ELECTROSTATIC ACCELERATORS; PROTON BEAMS; CELL CULTURES; IN VITRO; SURVIVAL CURVES; RADIATION INJURIES; EXTERNAL IRRADIATION; DNA; SOLUTIONS; STRAND BREAKS; PROTEINS; X RADIATION; CROSS-LINKING; BIOLOGICAL RADIATION EFFECTS
OSTI ID:
583912
Research Organizations:
Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany)
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Other: ON: DE98741859; TRN: DE98F5442
Availability:
INIS; OSTI as DE98741859
Submitting Site:
DEN
Size:
pp. D5.1-D5.4
Announcement Date:

Citation Formats

Distel, L, Distel, B, Roessner, B, Schwotzer, G, Sauer, R, Eyrich, W, Fritsch, M, Teufel, A, Besserer, J, Boer, J de, Moosburger, M, and Quicken, P. First biological experiments at a vertical proton beam. Germany: N. p., 1997. Web.
Distel, L, Distel, B, Roessner, B, Schwotzer, G, Sauer, R, Eyrich, W, Fritsch, M, Teufel, A, Besserer, J, Boer, J de, Moosburger, M, & Quicken, P. First biological experiments at a vertical proton beam. Germany.
Distel, L, Distel, B, Roessner, B, Schwotzer, G, Sauer, R, Eyrich, W, Fritsch, M, Teufel, A, Besserer, J, Boer, J de, Moosburger, M, and Quicken, P. 1997. "First biological experiments at a vertical proton beam." Germany.
@misc{etde_583912,
title = {First biological experiments at a vertical proton beam}
author = {Distel, L, Distel, B, Roessner, B, Schwotzer, G, Sauer, R, Eyrich, W, Fritsch, M, Teufel, A, Besserer, J, Boer, J de, Moosburger, M, and Quicken, P}
abstractNote = {At the tandem accelerator laboratories in Munich and Erlangen vertical beamlines were installed last year. The advantage of a vertical beamline is that cells can be irradiated in a medium at 37 C and with simultaneous gassing, therefore also in physiological conditions. First experiments were carried out at the accelerator in Munich with a proton energy of 25 MeV. Chinese Hamster cells B14 were irradiated in Petri dishes where the base was of 1 mm polystyrol or 2 {mu}m hostaphan foils. The cell survival was measured by the cell survival assay and the repopulation of the colonies by the total colony volume. A solution of DNA with protein was irradiated to study DNA double strand breaks by constant field gel electrophoresis and DNA protein crosslinks by the nitrocellulose filter assay. For cell survival, total colony volume and DNA double-strand breaks X-rays and protons gave corresponding results, while with protons, higher yields of DNA-protein crosslinks were observed than with X-rays. (orig.)}
place = {Germany}
year = {1997}
month = {Sep}
}