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Heavy ion-induced chromosomal aberrations analyzed by fluorescence in situ hybridization

Abstract

We have investigated the effectiveness of heavy ions in the induction of chromosomal aberrations in mammalian cells by the recent technique of fluorescence in situ hybridization (FISH) with whole-chromosome probes. FISH-painting was used both in metaphase and interphase (prematurely condensed) chromosomes. The purpose of our experiments was to address the following problems: (a) the ratio of different types of aberrations as a function of radiation quality (search for biomarkers); (b) the ratio between aberrations scored in interphase and metaphase as a function of radiation quality (role of apoptosis); (c) differences between cytogenetic effects produced by different ions at the same LET (role of track structure). (orig./MG)
Authors:
Durante, M; Gialanella, G; Grossi, G; Pugliese, M; [1]  Cella, L; Greco, O; [1]  Furusawa, Y; [2]  George, K; Yang, T C [3] 
  1. Univ. ``Federico II``, Naples (Italy). Dept. of Physics
  2. NIRS, Chiba (Japan)
  3. NASA Lyndon B. Johnson Space Center, Houston, TX (United States)
Publication Date:
Sep 01, 1997
Product Type:
Conference
Report Number:
GSI-97-09; CONF-9709224-
Reference Number:
SCA: 560120; PA: DEN-98:0F5455; EDB-98:035769; SN: 98001927291
Resource Relation:
Conference: 6. workshop on heavy-charged particles in biology and medicine, Baveno (Italy), 29 Sep - 1 Oct 1997; Other Information: PBD: Sep 1997; Related Information: Is Part Of 6. Workshop on heavy-charged particles in biology and medicine. Book of abstracts; Kraft, G.; Langbein, K. [eds.]; PB: 290 p.
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; MAMMALS; CELL CULTURES; EXTERNAL IRRADIATION; IN VITRO; HEAVY IONS; ION BEAMS; GENETIC RADIATION EFFECTS; CHROMOSOMAL ABERRATIONS; LET; CYTOLOGICAL TECHNIQUES; PARTICLE TRACKS; CELL KILLING; RADIATION QUALITY; GAMMA RADIATION; PROTONS
OSTI ID:
583908
Research Organizations:
Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany)
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Other: ON: DE98741859; TRN: DE98F5455
Availability:
INIS; OSTI as DE98741859
Submitting Site:
DEN
Size:
pp. C2.1-C2.4
Announcement Date:

Citation Formats

Durante, M, Gialanella, G, Grossi, G, Pugliese, M, Cella, L, Greco, O, Furusawa, Y, George, K, and Yang, T C. Heavy ion-induced chromosomal aberrations analyzed by fluorescence in situ hybridization. Germany: N. p., 1997. Web.
Durante, M, Gialanella, G, Grossi, G, Pugliese, M, Cella, L, Greco, O, Furusawa, Y, George, K, & Yang, T C. Heavy ion-induced chromosomal aberrations analyzed by fluorescence in situ hybridization. Germany.
Durante, M, Gialanella, G, Grossi, G, Pugliese, M, Cella, L, Greco, O, Furusawa, Y, George, K, and Yang, T C. 1997. "Heavy ion-induced chromosomal aberrations analyzed by fluorescence in situ hybridization." Germany.
@misc{etde_583908,
title = {Heavy ion-induced chromosomal aberrations analyzed by fluorescence in situ hybridization}
author = {Durante, M, Gialanella, G, Grossi, G, Pugliese, M, Cella, L, Greco, O, Furusawa, Y, George, K, and Yang, T C}
abstractNote = {We have investigated the effectiveness of heavy ions in the induction of chromosomal aberrations in mammalian cells by the recent technique of fluorescence in situ hybridization (FISH) with whole-chromosome probes. FISH-painting was used both in metaphase and interphase (prematurely condensed) chromosomes. The purpose of our experiments was to address the following problems: (a) the ratio of different types of aberrations as a function of radiation quality (search for biomarkers); (b) the ratio between aberrations scored in interphase and metaphase as a function of radiation quality (role of apoptosis); (c) differences between cytogenetic effects produced by different ions at the same LET (role of track structure). (orig./MG)}
place = {Germany}
year = {1997}
month = {Sep}
}