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Carbon ion induced DNA double-strand breaks in melanophore B{sub 16}

Abstract

DNA double-strand breaks (DSBs) in melanophore B{sub 16} induced by plateau and extended Bragg peak of 75 MeV/u {sup 12}C{sup 6+} ions were studied by using a technique of inverse pulsed-field gel electrophoresis (PIGE). DNA fragment lengths were distributed in two ranges: the larger in 1.4 Mbp-3.2 Mbp and the smaller in less than 1.2 Mbp. It indicates that distribution of DNA fragments induced by heavy ion irradiation is not stochastic and there probably are sensitive sites to heavy ions in DNA molecules of B{sub 16}. Percentage of DNA released from plug (PR) increased and trended towards a quasi-plateau {proportional_to}85% as dose increased. Content of the larger fragments decreased and flattened with increasing dose while content of the smaller ones increased and trended towards saturation. (orig.)
Authors:
Zengquan, Wei; Guangming, Zhou; Jufang, Wang; Jing, He; Qiang, Li; Wenjian, Li; Hongmei, Xie; Xichen, Cai; Huang, Tao; Bingrong, Dang; Guangwu, Han; [1]  Qingxiang, Gao [2] 
  1. Chinese Academy of Sciences, Lanzhou (China). Inst. of Modern Physics
  2. Lanzhou Univ. (China)
Publication Date:
Sep 01, 1997
Product Type:
Conference
Report Number:
GSI-97-09; CONF-9709224-
Reference Number:
SCA: 560120; PA: DEN-98:0F5464; EDB-98:035755; SN: 98001927288
Resource Relation:
Conference: 6. workshop on heavy-charged particles in biology and medicine, Baveno (Italy), 29 Sep - 1 Oct 1997; Other Information: PBD: Sep 1997; Related Information: Is Part Of 6. Workshop on heavy-charged particles in biology and medicine. Book of abstracts; Kraft, G.; Langbein, K. [eds.]; PB: 290 p.
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CARBON IONS; ION BEAMS; EXTERNAL IRRADIATION; BIOLOGICAL RADIATION EFFECTS; DNA; STRAND BREAKS; TUMOR CELLS; CELL CULTURES; IN VITRO; ELECTROPHORESIS; GELS
OSTI ID:
583905
Research Organizations:
Gesellschaft fuer Schwerionenforschung mbH, Darmstadt (Germany)
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Other: ON: DE98741859; TRN: DE98F5464
Availability:
INIS; OSTI as DE98741859
Submitting Site:
DEN
Size:
pp. A7.1-A7.4
Announcement Date:

Citation Formats

Zengquan, Wei, Guangming, Zhou, Jufang, Wang, Jing, He, Qiang, Li, Wenjian, Li, Hongmei, Xie, Xichen, Cai, Huang, Tao, Bingrong, Dang, Guangwu, Han, and Qingxiang, Gao. Carbon ion induced DNA double-strand breaks in melanophore B{sub 16}. Germany: N. p., 1997. Web.
Zengquan, Wei, Guangming, Zhou, Jufang, Wang, Jing, He, Qiang, Li, Wenjian, Li, Hongmei, Xie, Xichen, Cai, Huang, Tao, Bingrong, Dang, Guangwu, Han, & Qingxiang, Gao. Carbon ion induced DNA double-strand breaks in melanophore B{sub 16}. Germany.
Zengquan, Wei, Guangming, Zhou, Jufang, Wang, Jing, He, Qiang, Li, Wenjian, Li, Hongmei, Xie, Xichen, Cai, Huang, Tao, Bingrong, Dang, Guangwu, Han, and Qingxiang, Gao. 1997. "Carbon ion induced DNA double-strand breaks in melanophore B{sub 16}." Germany.
@misc{etde_583905,
title = {Carbon ion induced DNA double-strand breaks in melanophore B{sub 16}}
author = {Zengquan, Wei, Guangming, Zhou, Jufang, Wang, Jing, He, Qiang, Li, Wenjian, Li, Hongmei, Xie, Xichen, Cai, Huang, Tao, Bingrong, Dang, Guangwu, Han, and Qingxiang, Gao}
abstractNote = {DNA double-strand breaks (DSBs) in melanophore B{sub 16} induced by plateau and extended Bragg peak of 75 MeV/u {sup 12}C{sup 6+} ions were studied by using a technique of inverse pulsed-field gel electrophoresis (PIGE). DNA fragment lengths were distributed in two ranges: the larger in 1.4 Mbp-3.2 Mbp and the smaller in less than 1.2 Mbp. It indicates that distribution of DNA fragments induced by heavy ion irradiation is not stochastic and there probably are sensitive sites to heavy ions in DNA molecules of B{sub 16}. Percentage of DNA released from plug (PR) increased and trended towards a quasi-plateau {proportional_to}85% as dose increased. Content of the larger fragments decreased and flattened with increasing dose while content of the smaller ones increased and trended towards saturation. (orig.)}
place = {Germany}
year = {1997}
month = {Sep}
}