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Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid

Abstract

A method for evaluating an acellular biological fluid for the presence of a suspect viral DNA, such as DNA of the Hepatitis-B virus, is described. The acellular biological fluid is treated to immobilize in denatured form the DNAs including the suspect viral DNA on a solid substrate. This substrate is contacted with a solution including radioisotopically-labelled suspect viral denatured DNA to renature the immobilized suspect viral native DNA. The solid substrate is then evaluated for radioisotopically-labelled suspect viral renatured DNA.
Authors:
Publication Date:
Oct 06, 1982
Product Type:
Patent
Report Number:
GB 2095833; A
Reference Number:
AIX-14-778928; EDB-83-177840
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 62 RADIOLOGY AND NUCLEAR MEDICINE; DNA; DIAGNOSTIC TECHNIQUES; VIRUSES; BODY FLUIDS; HEPATITIS; HERPES SIMPLEX; IODINE 125; IODINE 131; LABELLED COMPOUNDS; MAN; NUCLEIC ACID DENATURATION; PHOSPHORUS 32; TRACER TECHNIQUES; TRITIUM; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM DISEASES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HYDROGEN ISOTOPES; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS ISOTOPES; PRIMATES; RADIOISOTOPES; SKIN DISEASES; VERTEBRATES; VIRAL DISEASES; YEARS LIVING RADIOISOTOPES; 400100* - Analytical & Separations Chemistry; 550601 - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5834191
Country of Origin:
United Kingdom
Language:
English
Submitting Site:
INIS
Size:
Pages: 3
Announcement Date:
May 13, 2001

Citation Formats

Berninger, M S. Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid. United Kingdom: N. p., 1982. Web.
Berninger, M S. Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid. United Kingdom.
Berninger, M S. 1982. "Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid." United Kingdom.
@misc{etde_5834191,
title = {Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid}
author = {Berninger, M S}
abstractNote = {A method for evaluating an acellular biological fluid for the presence of a suspect viral DNA, such as DNA of the Hepatitis-B virus, is described. The acellular biological fluid is treated to immobilize in denatured form the DNAs including the suspect viral DNA on a solid substrate. This substrate is contacted with a solution including radioisotopically-labelled suspect viral denatured DNA to renature the immobilized suspect viral native DNA. The solid substrate is then evaluated for radioisotopically-labelled suspect viral renatured DNA.}
place = {United Kingdom}
year = {1982}
month = {Oct}
}