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Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride

Abstract

The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl/sub 2/ at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus.
Authors:
Wakizaka, A; Okuhara, E [1] 
  1. Akita Univ. (Japan)
Publication Date:
Jan 23, 1979
Product Type:
Journal Article
Reference Number:
AIX-10-458768; EDB-79-132941
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 25:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; DNA; RADIOIMMUNOASSAY; GLOBULINS-GAMMA; LUPUS; DIAGNOSTIC TECHNIQUES; ANTIBODIES; ERYTHEMA; IMMUNE SERUMS; LABELLED COMPOUNDS; RABBITS; THYMIDINE; TRITIUM; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DISEASES; GLOBULINS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PROTEINS; PYRIMIDINES; RADIOASSAY; RADIOISOTOPES; RIBOSIDES; SKIN DISEASES; SYMPTOMS; TRACER TECHNIQUES; TUBERCULOSIS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5833736
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 119-125
Announcement Date:
Jun 01, 1979

Citation Formats

Wakizaka, A, and Okuhara, E. Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride. Netherlands: N. p., 1979. Web.
Wakizaka, A, & Okuhara, E. Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride. Netherlands.
Wakizaka, A, and Okuhara, E. 1979. "Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride." Netherlands.
@misc{etde_5833736,
title = {Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride}
author = {Wakizaka, A, and Okuhara, E}
abstractNote = {The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl/sub 2/ at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus.}
journal = []
volume = {25:2}
journal type = {AC}
place = {Netherlands}
year = {1979}
month = {Jan}
}