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Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemaglutination techniques for detecting cytomegalovirus IgG antibody

Abstract

The radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques were found to be comparable in sensitivity and specificity for detecting cytomegalovirus IgG antibody, and 10 to 100 times more sensitive than complement-fixation (CF), anticomplement immunofluorescence (ACIF) and passive haemagglutination (PHA). In screening tests for antibody, the frequency of false-positive and -negative results was 0.6% for RIA and ELISA, 1.5% for CF, 1.6% for ACIF and 3.6% for PHA. PHA was the least satisfactory test, largely because of technical problems.
Authors:
Booth, J C; Hannington, G; Bakir, T M.F.; Stern, H; Kangro, H; Griffiths, P D; Heath, R B; [1]  Saint Bartholomew's Hospital, London (UK))
  1. Saint George's Hospital Medical School, London (UK)
Publication Date:
Dec 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-14-778945; EDB-83-179589
Resource Relation:
Journal Name: J. Clin. Pathol.; (United Kingdom); Journal Volume: 35:12
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HEMAGGLUTININS; RADIOIMMUNOASSAY; VIRUSES; ANTIBODIES; BRAIN; COMPARATIVE EVALUATIONS; COMPLEMENT; DIAGNOSTIC TECHNIQUES; FLUORESCENCE; NEONATES; AGGLUTININS; BODY; CENTRAL NERVOUS SYSTEM; ISOTOPE APPLICATIONS; LUMINESCENCE; MICROORGANISMS; NERVOUS SYSTEM; ORGANS; PARASITES; RADIOASSAY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5820968
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JCPAA
Submitting Site:
HEDB
Size:
Pages: 1345-1348
Announcement Date:
Aug 01, 1983

Citation Formats

Booth, J C, Hannington, G, Bakir, T M.F., Stern, H, Kangro, H, Griffiths, P D, Heath, R B, and Saint Bartholomew's Hospital, London (UK)). Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemaglutination techniques for detecting cytomegalovirus IgG antibody. United Kingdom: N. p., 1982. Web.
Booth, J C, Hannington, G, Bakir, T M.F., Stern, H, Kangro, H, Griffiths, P D, Heath, R B, & Saint Bartholomew's Hospital, London (UK)). Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemaglutination techniques for detecting cytomegalovirus IgG antibody. United Kingdom.
Booth, J C, Hannington, G, Bakir, T M.F., Stern, H, Kangro, H, Griffiths, P D, Heath, R B, and Saint Bartholomew's Hospital, London (UK)). 1982. "Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemaglutination techniques for detecting cytomegalovirus IgG antibody." United Kingdom.
@misc{etde_5820968,
title = {Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemaglutination techniques for detecting cytomegalovirus IgG antibody}
author = {Booth, J C, Hannington, G, Bakir, T M.F., Stern, H, Kangro, H, Griffiths, P D, Heath, R B, and Saint Bartholomew's Hospital, London (UK))}
abstractNote = {The radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques were found to be comparable in sensitivity and specificity for detecting cytomegalovirus IgG antibody, and 10 to 100 times more sensitive than complement-fixation (CF), anticomplement immunofluorescence (ACIF) and passive haemagglutination (PHA). In screening tests for antibody, the frequency of false-positive and -negative results was 0.6% for RIA and ELISA, 1.5% for CF, 1.6% for ACIF and 3.6% for PHA. PHA was the least satisfactory test, largely because of technical problems.}
journal = []
volume = {35:12}
journal type = {AC}
place = {United Kingdom}
year = {1982}
month = {Dec}
}