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Differentiation and distribution of potato virus Y strains isolated from tobacco in South Africa

Thesis/Dissertation:

Abstract

Four strains of potato virus Y (PVY) orginally isolated from tobacco in South Africa belonging to three different strain groups (PVY/sup N/, PVY/sup c/ and PVY/sup o/) were differentiated according to their effect on various tobacco cultivars. The results obtained in this study confirm previous reports which indicated that inoculation with PVY had a detrimental effect on the yield and quality of tobacco. The severity of the effects was generally related to the length of time that the virus was present in the host, with late infections having less effect than early infections. An important aspect that evolved from the present study is the differences in reactions of the various strains (necrotic to mild strains) of PVY on the tobacco cultivars tested. A direct correlation was evident between the virulence of the different PVY strains and the effect of O/sub 2/-uptake of the host. cDNA probes prepared from PVY-RNA are specific to RNA extracted from purified PVY suspensions as well as crude sap from tobacco plants infected with the PVY strains used in this study. Radioactive probes and /sup 32/Phosporus labelling were used in the DNA and RNA studies of PVY. A procedure described by Bar-Joseph, et al (1983) were  More>>
Authors:
Publication Date:
Jan 01, 1986
Product Type:
Thesis/Dissertation
Reference Number:
AIX-19-001471; EDB-88-003727
Resource Relation:
Other Information: Thesis (M.Sc.)
Subject:
60 APPLIED LIFE SCIENCES; NICOTIANA; VIRAL DISEASES; VIRUSES; BIOLOGICAL VARIABILITY; STRAINS; DNA; ELECTROPHORESIS; EXPERIMENTAL DATA; HYBRIDIZATION; INOCULATION; LABELLING; NICOTINE; NITROGEN; OXYGEN; PHOSPHORUS 32; PLANT DISEASES; POTASSIUM; PROBES; RNA; SACCHARIDES; SOUTH AFRICA; SYNTHESIS; TOBACCO; ULTRAVIOLET SPECTRA; UPTAKE; AFRICA; ALKALI METALS; ALKALOIDS; AMINES; AUTONOMIC NERVOUS SYSTEM AGENTS; AZINES; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; DATA; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELEMENTS; HETEROCYCLIC COMPOUNDS; INFECTIOUS DISEASES; INFORMATION; ISOTOPES; LIGHT NUCLEI; METALS; MICROORGANISMS; NONMETALS; NUCLEI; NUCLEIC ACIDS; NUMERICAL DATA; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASITES; PARASYMPATHOLYTICS; PARASYMPATHOMIMETICS; PHOSPHORUS ISOTOPES; PLANTS; PYRIDINES; PYRROLES; PYRROLIDINES; RADIOISOTOPES; SPECTRA; 553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)
OSTI ID:
5820769
Research Organizations:
Pretoria Univ. (South Africa)
Country of Origin:
South Africa
Language:
English
Availability:
Registrar, University of Pretoria, Pretoria, 0002, South Africa.
Submitting Site:
INIS
Size:
Pages: 129
Announcement Date:

Thesis/Dissertation:

Citation Formats

Vorster, L L. Differentiation and distribution of potato virus Y strains isolated from tobacco in South Africa. South Africa: N. p., 1986. Web.
Vorster, L L. Differentiation and distribution of potato virus Y strains isolated from tobacco in South Africa. South Africa.
Vorster, L L. 1986. "Differentiation and distribution of potato virus Y strains isolated from tobacco in South Africa." South Africa.
@misc{etde_5820769,
title = {Differentiation and distribution of potato virus Y strains isolated from tobacco in South Africa}
author = {Vorster, L L}
abstractNote = {Four strains of potato virus Y (PVY) orginally isolated from tobacco in South Africa belonging to three different strain groups (PVY/sup N/, PVY/sup c/ and PVY/sup o/) were differentiated according to their effect on various tobacco cultivars. The results obtained in this study confirm previous reports which indicated that inoculation with PVY had a detrimental effect on the yield and quality of tobacco. The severity of the effects was generally related to the length of time that the virus was present in the host, with late infections having less effect than early infections. An important aspect that evolved from the present study is the differences in reactions of the various strains (necrotic to mild strains) of PVY on the tobacco cultivars tested. A direct correlation was evident between the virulence of the different PVY strains and the effect of O/sub 2/-uptake of the host. cDNA probes prepared from PVY-RNA are specific to RNA extracted from purified PVY suspensions as well as crude sap from tobacco plants infected with the PVY strains used in this study. Radioactive probes and /sup 32/Phosporus labelling were used in the DNA and RNA studies of PVY. A procedure described by Bar-Joseph, et al (1983) were used successfully for the isolation of viral double-stranded RNA from various tissues. However, from the results obtained in this study it is clear that this method is of little or no value for the detection and diagnosis of PVY strains.}
place = {South Africa}
year = {1986}
month = {Jan}
}