Abstract
8-Methoxypsoralen (8-MOP) when irradiated with long wavelength ultraviolet radiation (UV-A) inhibits DNA synthesis in lymphocytes in vitro and in vivo. 8-MOP binds reversibly to DNA in the dark; when exposed to UV-A, covalent monoadducts and cross-links are formed with the DNA. The present study correlates the cytotoxic effects of 8-MOP plus UV-A with DNA crosslinking. E-B virus transformed human lymphoblastoid cells were suspended in a colorless salt solution containing 8-MOP and exposed to UV-A from fluorescent lamps filtered to remove radiation below 320 nm (22.5 J/m/sup 2/-sec). Cells were then returned to complete medium and assayed for survival (by daily counts of viable cells and by cloning in microtiter wells) and for DNA crosslinking by alkaline elution. 8-MOP alone or UV-A alone resulted in minimal to no alterations in survivial or in DNA crosslinking. DNA crosslinking was found to be linearly dependent on 8-MOP concentration (in the range of 0.01-1.0 ..mu..g/ml) for 3 different UV-A doses (3000-15000 J/m/sup 2/). The surviving fraction declined exponentially as a function of the relative number of DNA crosslinks.
Cohen, L F;
Glaubiger, D L;
[1]
Kraemer, K H;
Waters, H L;
[2]
Kohn, K W
[3]
- National Cancer Inst., Bethesda, MD (USA). Pediatric Oncology Branch
- National Cancer Inst., Bethesda, MD (USA). Lab. of Molecular Carcinogenesis
- National Cancer Inst., Bethesda, MD (USA). Lab. of Molecular Pharmacology
Citation Formats
Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W.
DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation.
Netherlands: N. p.,
1981.
Web.
Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, & Kohn, K W.
DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation.
Netherlands.
Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W.
1981.
"DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation."
Netherlands.
@misc{etde_5820696,
title = {DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation}
author = {Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W}
abstractNote = {8-Methoxypsoralen (8-MOP) when irradiated with long wavelength ultraviolet radiation (UV-A) inhibits DNA synthesis in lymphocytes in vitro and in vivo. 8-MOP binds reversibly to DNA in the dark; when exposed to UV-A, covalent monoadducts and cross-links are formed with the DNA. The present study correlates the cytotoxic effects of 8-MOP plus UV-A with DNA crosslinking. E-B virus transformed human lymphoblastoid cells were suspended in a colorless salt solution containing 8-MOP and exposed to UV-A from fluorescent lamps filtered to remove radiation below 320 nm (22.5 J/m/sup 2/-sec). Cells were then returned to complete medium and assayed for survival (by daily counts of viable cells and by cloning in microtiter wells) and for DNA crosslinking by alkaline elution. 8-MOP alone or UV-A alone resulted in minimal to no alterations in survivial or in DNA crosslinking. DNA crosslinking was found to be linearly dependent on 8-MOP concentration (in the range of 0.01-1.0 ..mu..g/ml) for 3 different UV-A doses (3000-15000 J/m/sup 2/). The surviving fraction declined exponentially as a function of the relative number of DNA crosslinks.}
journal = []
volume = {80}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}
title = {DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation}
author = {Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W}
abstractNote = {8-Methoxypsoralen (8-MOP) when irradiated with long wavelength ultraviolet radiation (UV-A) inhibits DNA synthesis in lymphocytes in vitro and in vivo. 8-MOP binds reversibly to DNA in the dark; when exposed to UV-A, covalent monoadducts and cross-links are formed with the DNA. The present study correlates the cytotoxic effects of 8-MOP plus UV-A with DNA crosslinking. E-B virus transformed human lymphoblastoid cells were suspended in a colorless salt solution containing 8-MOP and exposed to UV-A from fluorescent lamps filtered to remove radiation below 320 nm (22.5 J/m/sup 2/-sec). Cells were then returned to complete medium and assayed for survival (by daily counts of viable cells and by cloning in microtiter wells) and for DNA crosslinking by alkaline elution. 8-MOP alone or UV-A alone resulted in minimal to no alterations in survivial or in DNA crosslinking. DNA crosslinking was found to be linearly dependent on 8-MOP concentration (in the range of 0.01-1.0 ..mu..g/ml) for 3 different UV-A doses (3000-15000 J/m/sup 2/). The surviving fraction declined exponentially as a function of the relative number of DNA crosslinks.}
journal = []
volume = {80}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}