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DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation

Journal Article:

Abstract

8-Methoxypsoralen (8-MOP) when irradiated with long wavelength ultraviolet radiation (UV-A) inhibits DNA synthesis in lymphocytes in vitro and in vivo. 8-MOP binds reversibly to DNA in the dark; when exposed to UV-A, covalent monoadducts and cross-links are formed with the DNA. The present study correlates the cytotoxic effects of 8-MOP plus UV-A with DNA crosslinking. E-B virus transformed human lymphoblastoid cells were suspended in a colorless salt solution containing 8-MOP and exposed to UV-A from fluorescent lamps filtered to remove radiation below 320 nm (22.5 J/m/sup 2/-sec). Cells were then returned to complete medium and assayed for survival (by daily counts of viable cells and by cloning in microtiter wells) and for DNA crosslinking by alkaline elution. 8-MOP alone or UV-A alone resulted in minimal to no alterations in survivial or in DNA crosslinking. DNA crosslinking was found to be linearly dependent on 8-MOP concentration (in the range of 0.01-1.0 ..mu..g/ml) for 3 different UV-A doses (3000-15000 J/m/sup 2/). The surviving fraction declined exponentially as a function of the relative number of DNA crosslinks.
Authors:
Cohen, L F; Glaubiger, D L; [1]  Kraemer, K H; Waters, H L; [2]  Kohn, K W [3] 
  1. National Cancer Inst., Bethesda, MD (USA). Pediatric Oncology Branch
  2. National Cancer Inst., Bethesda, MD (USA). Lab. of Molecular Carcinogenesis
  3. National Cancer Inst., Bethesda, MD (USA). Lab. of Molecular Pharmacology
Publication Date:
Jan 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-12-626832; EDB-82-032750
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 80
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; RADIOSENSITIVITY; PSORALEN; RESPONSE MODIFYING FACTORS; CELL CULTURES; CROSS-LINKING; DNA; IN VITRO; MAN; NEAR ULTRAVIOLET RADIATION; SURVIVAL CURVES; TOXICITY; ANIMAL CELLS; ANIMALS; ANTICOAGULANTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; COUMARINS; DRUGS; ELECTROMAGNETIC RADIATION; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; LEUKOCYTES; MAMMALS; MATERIALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; POLYMERIZATION; PRIMATES; RADIATIONS; SOMATIC CELLS; ULTRAVIOLET RADIATION; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
5820696
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
INIS
Size:
Pages: 347-356
Announcement Date:
Dec 01, 1981

Journal Article:

Citation Formats

Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W. DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation. Netherlands: N. p., 1981. Web.
Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, & Kohn, K W. DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation. Netherlands.
Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W. 1981. "DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation." Netherlands.
@misc{etde_5820696,
title = {DNA crosslinking and cell survival in human lymphoid cells treated with 8-methoxypsoralen and long wavelength ultraviolet radiation}
author = {Cohen, L F, Glaubiger, D L, Kraemer, K H, Waters, H L, and Kohn, K W}
abstractNote = {8-Methoxypsoralen (8-MOP) when irradiated with long wavelength ultraviolet radiation (UV-A) inhibits DNA synthesis in lymphocytes in vitro and in vivo. 8-MOP binds reversibly to DNA in the dark; when exposed to UV-A, covalent monoadducts and cross-links are formed with the DNA. The present study correlates the cytotoxic effects of 8-MOP plus UV-A with DNA crosslinking. E-B virus transformed human lymphoblastoid cells were suspended in a colorless salt solution containing 8-MOP and exposed to UV-A from fluorescent lamps filtered to remove radiation below 320 nm (22.5 J/m/sup 2/-sec). Cells were then returned to complete medium and assayed for survival (by daily counts of viable cells and by cloning in microtiter wells) and for DNA crosslinking by alkaline elution. 8-MOP alone or UV-A alone resulted in minimal to no alterations in survivial or in DNA crosslinking. DNA crosslinking was found to be linearly dependent on 8-MOP concentration (in the range of 0.01-1.0 ..mu..g/ml) for 3 different UV-A doses (3000-15000 J/m/sup 2/). The surviving fraction declined exponentially as a function of the relative number of DNA crosslinks.}
journal = {Mutat. Res.; (Netherlands)}
volume = {80}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}