Abstract
The Wide-Line NMR technique was used to determine the oil content in single corn seeds. Using distinct radio frequency (RF) power, a systematic work was done in kernels with about 10% of moisture, and also in artificially dried seeds with approximated 5% of moisture. For nondried seeds NMR spectra showed clearly the presence of three resonances with different RF saturation factor. For dried seeds, the oil concentration determined by NMR was highly correlated (r = 0,997) with that determined by a gravimetric method. The highest discrepancy between the two methods was found to be about 1,3%. When relative measurements are required as in the case of single kernel for recurrent selection program, precision in the individual selected kernel will be about 2,5%. Applying this technique, a first cycle of recurrent selection using S/sub 1/ lines for low and high oil content was performed in an open pollinated variety. Gain from selection was 12.0 and 14.1% in the populations for high and low oil contents, respectively.
Wilmers, M C.C.;
Rettori, C;
Vargas, H;
Barberis, G E;
[1]
da Silva, W J
[2]
- Universidade Estadual de Campinas (Brazil). Inst. de Fisica
- Universidade Estadual de Campinas (Brazil). Inst. de Biologia
Citation Formats
Wilmers, M C.C., Rettori, C, Vargas, H, Barberis, G E, and da Silva, W J.
Single corn kernel wide-line NMR oil analysis for breeding purpose.
Brazil: N. p.,
1978.
Web.
Wilmers, M C.C., Rettori, C, Vargas, H, Barberis, G E, & da Silva, W J.
Single corn kernel wide-line NMR oil analysis for breeding purpose.
Brazil.
Wilmers, M C.C., Rettori, C, Vargas, H, Barberis, G E, and da Silva, W J.
1978.
"Single corn kernel wide-line NMR oil analysis for breeding purpose."
Brazil.
@misc{etde_5796934,
title = {Single corn kernel wide-line NMR oil analysis for breeding purpose}
author = {Wilmers, M C.C., Rettori, C, Vargas, H, Barberis, G E, and da Silva, W J}
abstractNote = {The Wide-Line NMR technique was used to determine the oil content in single corn seeds. Using distinct radio frequency (RF) power, a systematic work was done in kernels with about 10% of moisture, and also in artificially dried seeds with approximated 5% of moisture. For nondried seeds NMR spectra showed clearly the presence of three resonances with different RF saturation factor. For dried seeds, the oil concentration determined by NMR was highly correlated (r = 0,997) with that determined by a gravimetric method. The highest discrepancy between the two methods was found to be about 1,3%. When relative measurements are required as in the case of single kernel for recurrent selection program, precision in the individual selected kernel will be about 2,5%. Applying this technique, a first cycle of recurrent selection using S/sub 1/ lines for low and high oil content was performed in an open pollinated variety. Gain from selection was 12.0 and 14.1% in the populations for high and low oil contents, respectively.}
journal = []
volume = {8:3}
journal type = {AC}
place = {Brazil}
year = {1978}
month = {Dec}
}
title = {Single corn kernel wide-line NMR oil analysis for breeding purpose}
author = {Wilmers, M C.C., Rettori, C, Vargas, H, Barberis, G E, and da Silva, W J}
abstractNote = {The Wide-Line NMR technique was used to determine the oil content in single corn seeds. Using distinct radio frequency (RF) power, a systematic work was done in kernels with about 10% of moisture, and also in artificially dried seeds with approximated 5% of moisture. For nondried seeds NMR spectra showed clearly the presence of three resonances with different RF saturation factor. For dried seeds, the oil concentration determined by NMR was highly correlated (r = 0,997) with that determined by a gravimetric method. The highest discrepancy between the two methods was found to be about 1,3%. When relative measurements are required as in the case of single kernel for recurrent selection program, precision in the individual selected kernel will be about 2,5%. Applying this technique, a first cycle of recurrent selection using S/sub 1/ lines for low and high oil content was performed in an open pollinated variety. Gain from selection was 12.0 and 14.1% in the populations for high and low oil contents, respectively.}
journal = []
volume = {8:3}
journal type = {AC}
place = {Brazil}
year = {1978}
month = {Dec}
}