Abstract
The quality control of insulin radioreceptor assay for human erythrocytes is based on the storage of erythrocyte preparations in Hepes buffer of pH 8.0, containing 10 g/l of albumin and 20 mmol/l of glucose. The change of erythrocytes into spherocytes and crenated cells reduces the apparent number of insulin receptors in a relatively constant way by less than 8% a week after 10 days of storage. At the same time the dissociation constants of the insulin-receptor complex increase rapidly. Thus the use of a preparation must be limited to controlling the determination of the insulin binding sites of erythrocytes, and not to the measurement of the affinities of the receptors. When mono-/sup 125/I-Tyr-A14-insulin gets old, a slow decrease in the insulin binding sites can be measured, but the dissociation constants of the insulin receptor complex are not affected.
Marttinen, A;
Pasternack, A;
[1]
Koivula, T;
Jokela, H;
Lehtinen, M
[2]
- Tampere Univ. (Finland). Dept. of Clinical Sciences
- Tampere Univ. Central Hospital (Finland). Dept. of Clinical Chemistry
Citation Formats
Marttinen, A, Pasternack, A, Koivula, T, Jokela, H, and Lehtinen, M.
Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-/sup 125/I-Tyr-A14-insulin preparation.
United Kingdom: N. p.,
1984.
Web.
doi:10.3109/00365518409083828.
Marttinen, A, Pasternack, A, Koivula, T, Jokela, H, & Lehtinen, M.
Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-/sup 125/I-Tyr-A14-insulin preparation.
United Kingdom.
https://doi.org/10.3109/00365518409083828
Marttinen, A, Pasternack, A, Koivula, T, Jokela, H, and Lehtinen, M.
1984.
"Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-/sup 125/I-Tyr-A14-insulin preparation."
United Kingdom.
https://doi.org/10.3109/00365518409083828.
@misc{etde_5754182,
title = {Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-/sup 125/I-Tyr-A14-insulin preparation}
author = {Marttinen, A, Pasternack, A, Koivula, T, Jokela, H, and Lehtinen, M}
abstractNote = {The quality control of insulin radioreceptor assay for human erythrocytes is based on the storage of erythrocyte preparations in Hepes buffer of pH 8.0, containing 10 g/l of albumin and 20 mmol/l of glucose. The change of erythrocytes into spherocytes and crenated cells reduces the apparent number of insulin receptors in a relatively constant way by less than 8% a week after 10 days of storage. At the same time the dissociation constants of the insulin-receptor complex increase rapidly. Thus the use of a preparation must be limited to controlling the determination of the insulin binding sites of erythrocytes, and not to the measurement of the affinities of the receptors. When mono-/sup 125/I-Tyr-A14-insulin gets old, a slow decrease in the insulin binding sites can be measured, but the dissociation constants of the insulin receptor complex are not affected.}
doi = {10.3109/00365518409083828}
journal = []
volume = {44:5}
journal type = {AC}
place = {United Kingdom}
year = {1984}
month = {Sep}
}
title = {Quality control of insulin radioreceptor assay for human erythrocytes. Effect of ageing of mono-/sup 125/I-Tyr-A14-insulin preparation}
author = {Marttinen, A, Pasternack, A, Koivula, T, Jokela, H, and Lehtinen, M}
abstractNote = {The quality control of insulin radioreceptor assay for human erythrocytes is based on the storage of erythrocyte preparations in Hepes buffer of pH 8.0, containing 10 g/l of albumin and 20 mmol/l of glucose. The change of erythrocytes into spherocytes and crenated cells reduces the apparent number of insulin receptors in a relatively constant way by less than 8% a week after 10 days of storage. At the same time the dissociation constants of the insulin-receptor complex increase rapidly. Thus the use of a preparation must be limited to controlling the determination of the insulin binding sites of erythrocytes, and not to the measurement of the affinities of the receptors. When mono-/sup 125/I-Tyr-A14-insulin gets old, a slow decrease in the insulin binding sites can be measured, but the dissociation constants of the insulin receptor complex are not affected.}
doi = {10.3109/00365518409083828}
journal = []
volume = {44:5}
journal type = {AC}
place = {United Kingdom}
year = {1984}
month = {Sep}
}