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Immunological measurements on the disappearance of creatine kinase MM from the circulation. [Immunoradiometric assay]

Abstract

Both a two-site immunoradiometric assay and a two-site enzyme-linked immunosorbent assay for creatine kinase MM are described. Linearity, reproducibility and cross-reactivity of the assays are satisfactory. Creatine kinase MM incubated in a pH-controlled serum matrix loses its activity, but has its antigenic determinants affected as well. Of all the techniques used, only the immunoradiometric assay is capable of measuring part of the inactivated enzyme. Measurements with this assay on the sera of patients after a myocardial infarction show identical results for enzyme activity and creatine kinase protein quantity. The in vitro disappearance rate of creatine kinase activity is slow compared with the in vivo half-life of the enzyme. These two observations lead to the conclusion that creatine kinase is removed from the circulation long before it is inactivated in the blood stream.
Authors:
Wevers, R A; van Landeghem, A A.J.; Mul-Steinbusch, M W.F.J.; Bijdendijk, J G; Weerts, P; Kloeg, P; Soons, J B.J. [1] 
  1. Rijksuniversiteit Utrecht (Netherlands)
Publication Date:
Jul 15, 1983
Product Type:
Journal Article
Reference Number:
AIX-14-792756; EDB-83-188361
Resource Relation:
Journal Name: Clin. Chim. Acta; (Netherlands); Journal Volume: 131:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PHOSPHOTRANSFERASES; RADIOASSAY; ACCURACY; COMPARATIVE EVALUATIONS; CREATINE; IMMUNE SERUMS; IODINE 125; LABELLED COMPOUNDS; SPECIFICITY; AMINO ACIDS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; RADIOISOTOPES; TRANSFERASES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5748584
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CCATA
Submitting Site:
HEDB
Size:
Pages: 193-200
Announcement Date:

Citation Formats

Wevers, R A, van Landeghem, A A.J., Mul-Steinbusch, M W.F.J., Bijdendijk, J G, Weerts, P, Kloeg, P, and Soons, J B.J. Immunological measurements on the disappearance of creatine kinase MM from the circulation. [Immunoradiometric assay]. Netherlands: N. p., 1983. Web. doi:10.1016/0009-8981(83)90088-8.
Wevers, R A, van Landeghem, A A.J., Mul-Steinbusch, M W.F.J., Bijdendijk, J G, Weerts, P, Kloeg, P, & Soons, J B.J. Immunological measurements on the disappearance of creatine kinase MM from the circulation. [Immunoradiometric assay]. Netherlands. doi:10.1016/0009-8981(83)90088-8.
Wevers, R A, van Landeghem, A A.J., Mul-Steinbusch, M W.F.J., Bijdendijk, J G, Weerts, P, Kloeg, P, and Soons, J B.J. 1983. "Immunological measurements on the disappearance of creatine kinase MM from the circulation. [Immunoradiometric assay]." Netherlands. doi:10.1016/0009-8981(83)90088-8. https://www.osti.gov/servlets/purl/10.1016/0009-8981(83)90088-8.
@misc{etde_5748584,
title = {Immunological measurements on the disappearance of creatine kinase MM from the circulation. [Immunoradiometric assay]}
author = {Wevers, R A, van Landeghem, A A.J., Mul-Steinbusch, M W.F.J., Bijdendijk, J G, Weerts, P, Kloeg, P, and Soons, J B.J.}
abstractNote = {Both a two-site immunoradiometric assay and a two-site enzyme-linked immunosorbent assay for creatine kinase MM are described. Linearity, reproducibility and cross-reactivity of the assays are satisfactory. Creatine kinase MM incubated in a pH-controlled serum matrix loses its activity, but has its antigenic determinants affected as well. Of all the techniques used, only the immunoradiometric assay is capable of measuring part of the inactivated enzyme. Measurements with this assay on the sera of patients after a myocardial infarction show identical results for enzyme activity and creatine kinase protein quantity. The in vitro disappearance rate of creatine kinase activity is slow compared with the in vivo half-life of the enzyme. These two observations lead to the conclusion that creatine kinase is removed from the circulation long before it is inactivated in the blood stream.}
doi = {10.1016/0009-8981(83)90088-8}
journal = {Clin. Chim. Acta; (Netherlands)}
volume = {131:3}
journal type = {AC}
place = {Netherlands}
year = {1983}
month = {Jul}
}