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(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors

Journal Article:

Abstract

(/sup 3/H)Spiperone ((/sup 3/H)SP) binding sites were localized by light microscopic autoradiography, after in vitro labelling. The kinetic and pharmacological characteristics of these binding sites were studied in slide-mounted sections of rat forebrain, and optimal labeling conditions were defined. Autoradiograms were obtained by apposing emulsion-coated coverslips to labeled sections. Differential drug sensitivity allowed the selective displacement of (/sup 3/H)SP from dopamine receptors by ADTN, from serotonin receptors by cinanserin, from both by haloperidol and from unique spiperone sites by unlabeled spiperone. The various sites presented a differential anatomical localization. For example, only dopaminergic sites were found in the glomerular layer of the olfactory bulb; only serotonergic sites were found in lamina IV of the neocortex, and a high concentration of unique spiperone sites were found in parts of the hippocampus.
Authors:
Palacios, J M; Niehoff, D L; Kuhar, M J [1] 
  1. Johns Hopkins Univ., Baltimore, MD (USA). School of Medicine
Publication Date:
Jan 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-12-626993; EDB-82-050919
Resource Relation:
Journal Name: Brain Res.; (Netherlands); Journal Volume: 213:2
Subject:
59 BASIC BIOLOGICAL SCIENCES; HORMONES; RECEPTORS; AUTORADIOGRAPHY; BRAIN; LABELLED COMPOUNDS; RATS; TRITIUM COMPOUNDS; ANIMALS; BODY; CENTRAL NERVOUS SYSTEM; MAMMALS; NERVOUS SYSTEM; ORGANS; RODENTS; VERTEBRATES; 550901* - Pathology- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques
OSTI ID:
5727592
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BRREA
Submitting Site:
INIS
Size:
Pages: 277-289
Announcement Date:

Journal Article:

Citation Formats

Palacios, J M, Niehoff, D L, and Kuhar, M J. (/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors. Netherlands: N. p., 1981. Web.
Palacios, J M, Niehoff, D L, & Kuhar, M J. (/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors. Netherlands.
Palacios, J M, Niehoff, D L, and Kuhar, M J. 1981. "(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors." Netherlands.
@misc{etde_5727592,
title = {(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors}
author = {Palacios, J M, Niehoff, D L, and Kuhar, M J}
abstractNote = {(/sup 3/H)Spiperone ((/sup 3/H)SP) binding sites were localized by light microscopic autoradiography, after in vitro labelling. The kinetic and pharmacological characteristics of these binding sites were studied in slide-mounted sections of rat forebrain, and optimal labeling conditions were defined. Autoradiograms were obtained by apposing emulsion-coated coverslips to labeled sections. Differential drug sensitivity allowed the selective displacement of (/sup 3/H)SP from dopamine receptors by ADTN, from serotonin receptors by cinanserin, from both by haloperidol and from unique spiperone sites by unlabeled spiperone. The various sites presented a differential anatomical localization. For example, only dopaminergic sites were found in the glomerular layer of the olfactory bulb; only serotonergic sites were found in lamina IV of the neocortex, and a high concentration of unique spiperone sites were found in parts of the hippocampus.}
journal = {Brain Res.; (Netherlands)}
volume = {213:2}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}