Abstract
(/sup 3/H)Spiperone ((/sup 3/H)SP) binding sites were localized by light microscopic autoradiography, after in vitro labelling. The kinetic and pharmacological characteristics of these binding sites were studied in slide-mounted sections of rat forebrain, and optimal labeling conditions were defined. Autoradiograms were obtained by apposing emulsion-coated coverslips to labeled sections. Differential drug sensitivity allowed the selective displacement of (/sup 3/H)SP from dopamine receptors by ADTN, from serotonin receptors by cinanserin, from both by haloperidol and from unique spiperone sites by unlabeled spiperone. The various sites presented a differential anatomical localization. For example, only dopaminergic sites were found in the glomerular layer of the olfactory bulb; only serotonergic sites were found in lamina IV of the neocortex, and a high concentration of unique spiperone sites were found in parts of the hippocampus.
Palacios, J M;
Niehoff, D L;
Kuhar, M J
[1]
- Johns Hopkins Univ., Baltimore, MD (USA). School of Medicine
Citation Formats
Palacios, J M, Niehoff, D L, and Kuhar, M J.
(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors.
Netherlands: N. p.,
1981.
Web.
Palacios, J M, Niehoff, D L, & Kuhar, M J.
(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors.
Netherlands.
Palacios, J M, Niehoff, D L, and Kuhar, M J.
1981.
"(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors."
Netherlands.
@misc{etde_5727592,
title = {(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors}
author = {Palacios, J M, Niehoff, D L, and Kuhar, M J}
abstractNote = {(/sup 3/H)Spiperone ((/sup 3/H)SP) binding sites were localized by light microscopic autoradiography, after in vitro labelling. The kinetic and pharmacological characteristics of these binding sites were studied in slide-mounted sections of rat forebrain, and optimal labeling conditions were defined. Autoradiograms were obtained by apposing emulsion-coated coverslips to labeled sections. Differential drug sensitivity allowed the selective displacement of (/sup 3/H)SP from dopamine receptors by ADTN, from serotonin receptors by cinanserin, from both by haloperidol and from unique spiperone sites by unlabeled spiperone. The various sites presented a differential anatomical localization. For example, only dopaminergic sites were found in the glomerular layer of the olfactory bulb; only serotonergic sites were found in lamina IV of the neocortex, and a high concentration of unique spiperone sites were found in parts of the hippocampus.}
journal = []
volume = {213:2}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}
title = {(/sup 3/H)Spiperone binding sites in brain: autoradiographic localization of multiple receptors}
author = {Palacios, J M, Niehoff, D L, and Kuhar, M J}
abstractNote = {(/sup 3/H)Spiperone ((/sup 3/H)SP) binding sites were localized by light microscopic autoradiography, after in vitro labelling. The kinetic and pharmacological characteristics of these binding sites were studied in slide-mounted sections of rat forebrain, and optimal labeling conditions were defined. Autoradiograms were obtained by apposing emulsion-coated coverslips to labeled sections. Differential drug sensitivity allowed the selective displacement of (/sup 3/H)SP from dopamine receptors by ADTN, from serotonin receptors by cinanserin, from both by haloperidol and from unique spiperone sites by unlabeled spiperone. The various sites presented a differential anatomical localization. For example, only dopaminergic sites were found in the glomerular layer of the olfactory bulb; only serotonergic sites were found in lamina IV of the neocortex, and a high concentration of unique spiperone sites were found in parts of the hippocampus.}
journal = []
volume = {213:2}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}