Abstract
Four normal adults were given (/sup 15/N)-glycine in a single dose either orally or intravenously. Rates of whole-body protein turnover were estimated from the excretion of /sup 15/N in ammonia and in urea during the following 9 h. The rate derived from urea took account of the (/sup 15/N)urea retained in body water. In postabsorptive subjects the rates of protein synthesis given by ammonia were equal to those from urea, when the isotope was given orally, but lower when an intravenous dose was given. In subjects receiving equal portions of food every 2 h rates of synthesis calculated from ammonia were much lower than those from urea whether an oral or intravenous isotope was given. Comparison of rates obtained during the postabsorptive and absorptive periods indicated regulation by food intake primarily of synthesis when measurements were made on urea, but regulation primarily of breakdown when measurements were made on ammonia. These inconsistencies suggest that changes in protein metabolism might be assessed better by correlating results given by different end-products, and it is suggested that the mean value given by urea and ammonia will be useful for this purpose.
Fern, E B;
Garlick, P J;
McNurlan, M A;
Waterlow, J C
[1]
- London School of Hygiene and Tropical Medicine (UK)
Citation Formats
Fern, E B, Garlick, P J, McNurlan, M A, and Waterlow, J C.
Excretion of isotope in urea and ammonia for estimating protein turnover in man with (/sup 15/N)glycine.
United Kingdom: N. p.,
1981.
Web.
Fern, E B, Garlick, P J, McNurlan, M A, & Waterlow, J C.
Excretion of isotope in urea and ammonia for estimating protein turnover in man with (/sup 15/N)glycine.
United Kingdom.
Fern, E B, Garlick, P J, McNurlan, M A, and Waterlow, J C.
1981.
"Excretion of isotope in urea and ammonia for estimating protein turnover in man with (/sup 15/N)glycine."
United Kingdom.
@misc{etde_5714841,
title = {Excretion of isotope in urea and ammonia for estimating protein turnover in man with (/sup 15/N)glycine}
author = {Fern, E B, Garlick, P J, McNurlan, M A, and Waterlow, J C}
abstractNote = {Four normal adults were given (/sup 15/N)-glycine in a single dose either orally or intravenously. Rates of whole-body protein turnover were estimated from the excretion of /sup 15/N in ammonia and in urea during the following 9 h. The rate derived from urea took account of the (/sup 15/N)urea retained in body water. In postabsorptive subjects the rates of protein synthesis given by ammonia were equal to those from urea, when the isotope was given orally, but lower when an intravenous dose was given. In subjects receiving equal portions of food every 2 h rates of synthesis calculated from ammonia were much lower than those from urea whether an oral or intravenous isotope was given. Comparison of rates obtained during the postabsorptive and absorptive periods indicated regulation by food intake primarily of synthesis when measurements were made on urea, but regulation primarily of breakdown when measurements were made on ammonia. These inconsistencies suggest that changes in protein metabolism might be assessed better by correlating results given by different end-products, and it is suggested that the mean value given by urea and ammonia will be useful for this purpose.}
journal = []
volume = {61:2}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {Jan}
}
title = {Excretion of isotope in urea and ammonia for estimating protein turnover in man with (/sup 15/N)glycine}
author = {Fern, E B, Garlick, P J, McNurlan, M A, and Waterlow, J C}
abstractNote = {Four normal adults were given (/sup 15/N)-glycine in a single dose either orally or intravenously. Rates of whole-body protein turnover were estimated from the excretion of /sup 15/N in ammonia and in urea during the following 9 h. The rate derived from urea took account of the (/sup 15/N)urea retained in body water. In postabsorptive subjects the rates of protein synthesis given by ammonia were equal to those from urea, when the isotope was given orally, but lower when an intravenous dose was given. In subjects receiving equal portions of food every 2 h rates of synthesis calculated from ammonia were much lower than those from urea whether an oral or intravenous isotope was given. Comparison of rates obtained during the postabsorptive and absorptive periods indicated regulation by food intake primarily of synthesis when measurements were made on urea, but regulation primarily of breakdown when measurements were made on ammonia. These inconsistencies suggest that changes in protein metabolism might be assessed better by correlating results given by different end-products, and it is suggested that the mean value given by urea and ammonia will be useful for this purpose.}
journal = []
volume = {61:2}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {Jan}
}