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Non-circadian rhythm in proliferation of haematopoietic stem cells

Journal Article:

Abstract

The proportion of haematopoietic stem cells (CFU-s) engaged in DNA synthesis was determined by means of the (/sup 3/H)-thymidine ((/sup 3/H)TdR) suicide technique during recovery of bone marrow from the damage caused by a sublethal total body irradiation. In contrast with previous reports the (/sup 3/H)TdR suicide rate was not permanently increased. It was observed that CFU-s passed through S phase in synchronous waves, following a dose of irradiation of 1.5 Gy. After a dose of 2.6 Gy, there was only one initial wave of increased CFU-s sensitivity to the action of (/sup 3/H)TdR. Following the depression occurring 26 hr after the irradiation with 2.6 Gy, the proportion of CFU-s killed by the (/sup 3/H)TdR was permanently increased until 5-6 days after irradiation. Thereafter large differences in the (/sup 3/H)TdR suicide data were observed among individual mice. Evidence was obtained that individual mice, which had been irradiated by a dose of 2.6 Gy 8-9 days before, had identical values of the CFU-s (/sup 3/H)TdR suicide rate in the bone marrow from different bones of the lower extremities. The recurrence of the synchronous waves in CFU-s passage through the cell cycle was recorded when the CFU-s population regenerated to only about  More>>
Authors:
Necas, E; Znojil, V [1] 
  1. Charles Univ., Prague (Czechoslovakia). Faculty of Medicine
Publication Date:
Mar 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-20-075710; EDB-89-147967
Resource Relation:
Journal Name: Cell and Tissue Kinetics; (UK); Journal Volume: 21:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CFU; BIOLOGICAL RADIATION EFFECTS; CELL PROLIFERATION; WHOLE-BODY IRRADIATION; STEM CELLS; BONE MARROW; GAMMA RADIATION; MICE; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BIOLOGICAL EFFECTS; BODY; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; IONIZING RADIATIONS; IRRADIATION; MAMMALS; ORGANS; RADIATION EFFECTS; RADIATIONS; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
5714289
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0008-8730; CODEN: CTKIA
Submitting Site:
GBN
Size:
Pages: 73-80
Announcement Date:

Journal Article:

Citation Formats

Necas, E, and Znojil, V. Non-circadian rhythm in proliferation of haematopoietic stem cells. United Kingdom: N. p., 1988. Web.
Necas, E, & Znojil, V. Non-circadian rhythm in proliferation of haematopoietic stem cells. United Kingdom.
Necas, E, and Znojil, V. 1988. "Non-circadian rhythm in proliferation of haematopoietic stem cells." United Kingdom.
@misc{etde_5714289,
title = {Non-circadian rhythm in proliferation of haematopoietic stem cells}
author = {Necas, E, and Znojil, V}
abstractNote = {The proportion of haematopoietic stem cells (CFU-s) engaged in DNA synthesis was determined by means of the (/sup 3/H)-thymidine ((/sup 3/H)TdR) suicide technique during recovery of bone marrow from the damage caused by a sublethal total body irradiation. In contrast with previous reports the (/sup 3/H)TdR suicide rate was not permanently increased. It was observed that CFU-s passed through S phase in synchronous waves, following a dose of irradiation of 1.5 Gy. After a dose of 2.6 Gy, there was only one initial wave of increased CFU-s sensitivity to the action of (/sup 3/H)TdR. Following the depression occurring 26 hr after the irradiation with 2.6 Gy, the proportion of CFU-s killed by the (/sup 3/H)TdR was permanently increased until 5-6 days after irradiation. Thereafter large differences in the (/sup 3/H)TdR suicide data were observed among individual mice. Evidence was obtained that individual mice, which had been irradiated by a dose of 2.6 Gy 8-9 days before, had identical values of the CFU-s (/sup 3/H)TdR suicide rate in the bone marrow from different bones of the lower extremities. The recurrence of the synchronous waves in CFU-s passage through the cell cycle was recorded when the CFU-s population regenerated to only about 10% of its normal value. It is concluded that the synchronous waves in which CFU-s proliferation occurred reflected the action of the control mechanism on CFU-s proliferation. (author).}
journal = {Cell and Tissue Kinetics; (UK)}
volume = {21:2}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Mar}
}