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Quantitation of specific myeloid cells in rat bone marrow measured by in vitro /sup 35/S-sulphate incorporation

Abstract

A biochemical measurement which can be used for quantitation of specific early myeloid cells in rat bone marrow has been developed. This measurement consists of a rapid, simple assay for the in vitro quantitation of /sup 35/S-sulfate incorporation into rat bone marrow cells. Incubation of bone marrow cells with /sup 35/S-sulfate led to a time-dependent increase in radioactivity obtained in perchloric acid insoluble fractions of bone marrow cell suspensions. This incorporation was inhibited by cyanide and puromycin. Autoradiography has demonstrated the radiolabel to be specifically associated with immature cells of the myeloid series. The cells most active in this respect were eosinophils. When rats were treated with endotoxin, the rate of /sup 35/S-sulfate incorporation was increased. Cell number measurements, using conventional histopathology and a Coulter Counter, demonstrated that endotoxin caused an initial release of mature granulocytes from the bone marrow. The regeneration of this mature population in the marrow was rapid, and was characterized by an increase in the number of immature cells and a concomitant increase in the rate of /sup 35/S-sulfate incorporation measured in preparations of bone marrow cells in vitro. Furthermore, this response to endotoxin has demonstrated that Coulter Counting techniques can be used to distinguish specific  More>>
Publication Date:
Aug 01, 1984
Product Type:
Journal Article
Reference Number:
EDB-85-088310
Resource Relation:
Journal Name: JAT, J. Appl. Toxicol.; (United Kingdom); Journal Volume: 4:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; BONE MARROW; AUTORADIOGRAPHY; CYTOLOGY; SULFUR 35; UPTAKE; BONE MARROW CELLS; ENDOTOXINS; IN VITRO; LABELLED COMPOUNDS; RATS; SULFATES; TIME DEPENDENCE; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGY; BODY; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; HEMATOPOIETIC SYSTEM; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; NUCLEI; ORGANS; OXYGEN COMPOUNDS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; SULFUR COMPOUNDS; SULFUR ISOTOPES; TISSUES; TOXIC MATERIALS; TOXINS; VERTEBRATES; 550301* - Cytology- Tracer Techniques
OSTI ID:
5709849
Research Organizations:
Imperial Chemical Industries PLC, Macclesfield, Cheshire, England
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JJATD
Submitting Site:
HEDB
Size:
Pages: 211-218
Announcement Date:

Citation Formats

Wright, A F, and Rose, M S. Quantitation of specific myeloid cells in rat bone marrow measured by in vitro /sup 35/S-sulphate incorporation. United Kingdom: N. p., 1984. Web. doi:10.1002/jat.2550040410.
Wright, A F, & Rose, M S. Quantitation of specific myeloid cells in rat bone marrow measured by in vitro /sup 35/S-sulphate incorporation. United Kingdom. doi:10.1002/jat.2550040410.
Wright, A F, and Rose, M S. 1984. "Quantitation of specific myeloid cells in rat bone marrow measured by in vitro /sup 35/S-sulphate incorporation." United Kingdom. doi:10.1002/jat.2550040410. https://www.osti.gov/servlets/purl/10.1002/jat.2550040410.
@misc{etde_5709849,
title = {Quantitation of specific myeloid cells in rat bone marrow measured by in vitro /sup 35/S-sulphate incorporation}
author = {Wright, A F, and Rose, M S}
abstractNote = {A biochemical measurement which can be used for quantitation of specific early myeloid cells in rat bone marrow has been developed. This measurement consists of a rapid, simple assay for the in vitro quantitation of /sup 35/S-sulfate incorporation into rat bone marrow cells. Incubation of bone marrow cells with /sup 35/S-sulfate led to a time-dependent increase in radioactivity obtained in perchloric acid insoluble fractions of bone marrow cell suspensions. This incorporation was inhibited by cyanide and puromycin. Autoradiography has demonstrated the radiolabel to be specifically associated with immature cells of the myeloid series. The cells most active in this respect were eosinophils. When rats were treated with endotoxin, the rate of /sup 35/S-sulfate incorporation was increased. Cell number measurements, using conventional histopathology and a Coulter Counter, demonstrated that endotoxin caused an initial release of mature granulocytes from the bone marrow. The regeneration of this mature population in the marrow was rapid, and was characterized by an increase in the number of immature cells and a concomitant increase in the rate of /sup 35/S-sulfate incorporation measured in preparations of bone marrow cells in vitro. Furthermore, this response to endotoxin has demonstrated that Coulter Counting techniques can be used to distinguish specific populations of cells (e.g. mature granulocytes) within the bone marrow.}
doi = {10.1002/jat.2550040410}
journal = {JAT, J. Appl. Toxicol.; (United Kingdom)}
volume = {4:4}
journal type = {AC}
place = {United Kingdom}
year = {1984}
month = {Aug}
}