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Sensitive method for the assay of guanylate cyclase activity

Abstract

A method for the assay of guanylate cyclase is described utilizing ..cap alpha..-(/sup 32/P)-GTP as substrate for the enzyme reaction. 100-150 ..mu..g of enzyme protein is incubated in a 15.6 mM Tris-HCl buffer incubation mixture, pH 7.6. The reaction is stopped by the addition of EDTA. The (/sup 32/P)-cyclic GMP formed is separated by a two-step column chromatography on Dowex 50W-X4 ion-exchange resin and neutral alumina. The recovery for cyclic GMP was about 70%. The blank values ranged from 0.001-0.003 % of the added ..cap alpha..-(/sup 32/P)-GTP which had been purified by Dowex 50W-X4 column chromatography. This method was employed for the assay of guanylate cyclase activities in different tissues.
Authors:
Karczewski, P; Krause, E G [1] 
  1. Akademie der Wissenschaften der DDR, Berlin-Buch. Zentralinstitut fuer Herz- und Kreislauf-Regulationsforschung
Publication Date:
Jul 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-10-469876; EDB-80-006587
Resource Relation:
Journal Name: Acta Biol. Med. Ger.; (German Democratic Republic); Journal Volume: 37:7
Subject:
59 BASIC BIOLOGICAL SCIENCES; GUANOSINE; RADIOASSAY; BIOCHEMISTRY; CATALYSIS; GUANYLIC ACID; HOMOGENATES; LABELLED COMPOUNDS; LYASES; METABOLISM; PHOSPHORUS 32; TISSUES; TRITIUM COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CHEMISTRY; DAYS LIVING RADIOISOTOPES; ENZYMES; HETEROCYCLIC COMPOUNDS; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOSPHORUS ISOTOPES; PURINES; RADIOISOTOPES; RIBOSIDES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
5704450
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ABMGA
Submitting Site:
INIS
Size:
Pages: 961-967
Announcement Date:
Sep 01, 1979

Citation Formats

Karczewski, P, and Krause, E G. Sensitive method for the assay of guanylate cyclase activity. Germany: N. p., 1978. Web.
Karczewski, P, & Krause, E G. Sensitive method for the assay of guanylate cyclase activity. Germany.
Karczewski, P, and Krause, E G. 1978. "Sensitive method for the assay of guanylate cyclase activity." Germany.
@misc{etde_5704450,
title = {Sensitive method for the assay of guanylate cyclase activity}
author = {Karczewski, P, and Krause, E G}
abstractNote = {A method for the assay of guanylate cyclase is described utilizing ..cap alpha..-(/sup 32/P)-GTP as substrate for the enzyme reaction. 100-150 ..mu..g of enzyme protein is incubated in a 15.6 mM Tris-HCl buffer incubation mixture, pH 7.6. The reaction is stopped by the addition of EDTA. The (/sup 32/P)-cyclic GMP formed is separated by a two-step column chromatography on Dowex 50W-X4 ion-exchange resin and neutral alumina. The recovery for cyclic GMP was about 70%. The blank values ranged from 0.001-0.003 % of the added ..cap alpha..-(/sup 32/P)-GTP which had been purified by Dowex 50W-X4 column chromatography. This method was employed for the assay of guanylate cyclase activities in different tissues.}
journal = []
volume = {37:7}
journal type = {AC}
place = {Germany}
year = {1978}
month = {Jul}
}