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Studies on the propagation in cell culture and the infectivity for baboons of human hepatitis A virus

Thesis/Dissertation:

Abstract

Current aspects of hepatitis A and hepatitis A virus (HAV) research and the techniques used for the propagation and monitoring of HAV and HAV antigen (HA Ag) production in vitro and HAV infection in vivo, and its sequelae are reviewed. Radioimmunoassay, immunofluorescence and electron microscopic techniques for the demonstration of HA Ag were adapted for this investigation. The cell-adapted strain of HAV(MBB) was successfully propagated in the human hepatoma cell line PLC/PRF/5 at 32 degrees Celsius. A crystalline structure was demonstrated in the cytoplasm of HAV-infected cells by thin-section electron microscopy. The origin and significance of this structure is uncertain. A possible temperature variant of HAV (strain MBB) or an HAV-related baboon virus was detected in PLC/PRF/5 cells maintained at 37 degrees Celsius after infection with a faecal extract prepared from baboons which had been infected with the cell-cultured HAV. Baboons, both free-ranging and in captivity, were found to have antibodies to HAV, which suggests susceptibility to human HAV or another cross-reacting virus. The experimental infection of the Cape baboon orally, intravenously or by both routes with HAV were investigated. The results of the study suggest reasons for the presence of anti-HAV antibodies in certain baboon populations and show that  More>>
Authors:
Publication Date:
Jan 01, 1985
Product Type:
Thesis/Dissertation
Reference Number:
AIX-17-031643; EDB-86-111652
Resource Relation:
Other Information: Thesis (Ph.D.)
Subject:
59 BASIC BIOLOGICAL SCIENCES; BABOONS; INFECTIOUS HEPATITIS; INFECTIVITY; VIRUSES; RADIOIMMUNOASSAY; ANTIBODIES; ANTIGENS; CELL CULTURES; DISEASE VECTORS; ELECTRON MICROSCOPY; EXPERIMENTAL DATA; FLUORESCENCE; GROWTH; IMMUNE SERUMS; IN VITRO; IN VIVO; INDIUM 125; MAN; STRAINS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DATA; DISEASES; IMMUNOASSAY; IMMUNOLOGY; INDIUM ISOTOPES; INFECTIOUS DISEASES; INFORMATION; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LUMINESCENCE; MAMMALS; MICROORGANISMS; MICROSCOPY; MONKEYS; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; PARASITES; PRIMATES; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; TRACER TECHNIQUES; VERTEBRATES; VIRAL DISEASES; 550701* - Microbiology- Tracer Techniques
OSTI ID:
5687433
Research Organizations:
Pretoria Univ. (South Africa)
Country of Origin:
South Africa
Language:
English
Availability:
Registrar, University of Pretoria, Pretoria, 0002, South Africa.
Submitting Site:
INIS
Size:
Pages: 164
Announcement Date:

Thesis/Dissertation:

Citation Formats

Taylor, M B. Studies on the propagation in cell culture and the infectivity for baboons of human hepatitis A virus. South Africa: N. p., 1985. Web.
Taylor, M B. Studies on the propagation in cell culture and the infectivity for baboons of human hepatitis A virus. South Africa.
Taylor, M B. 1985. "Studies on the propagation in cell culture and the infectivity for baboons of human hepatitis A virus." South Africa.
@misc{etde_5687433,
title = {Studies on the propagation in cell culture and the infectivity for baboons of human hepatitis A virus}
author = {Taylor, M B}
abstractNote = {Current aspects of hepatitis A and hepatitis A virus (HAV) research and the techniques used for the propagation and monitoring of HAV and HAV antigen (HA Ag) production in vitro and HAV infection in vivo, and its sequelae are reviewed. Radioimmunoassay, immunofluorescence and electron microscopic techniques for the demonstration of HA Ag were adapted for this investigation. The cell-adapted strain of HAV(MBB) was successfully propagated in the human hepatoma cell line PLC/PRF/5 at 32 degrees Celsius. A crystalline structure was demonstrated in the cytoplasm of HAV-infected cells by thin-section electron microscopy. The origin and significance of this structure is uncertain. A possible temperature variant of HAV (strain MBB) or an HAV-related baboon virus was detected in PLC/PRF/5 cells maintained at 37 degrees Celsius after infection with a faecal extract prepared from baboons which had been infected with the cell-cultured HAV. Baboons, both free-ranging and in captivity, were found to have antibodies to HAV, which suggests susceptibility to human HAV or another cross-reacting virus. The experimental infection of the Cape baboon orally, intravenously or by both routes with HAV were investigated. The results of the study suggest reasons for the presence of anti-HAV antibodies in certain baboon populations and show that the baboon is not an ideal model for hepatitis A investigations.}
place = {South Africa}
year = {1985}
month = {Jan}
}