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Storage of human platelets by freezing

Journal Article:

Abstract

Prolonged, probably indefinite storage of viable and functional human platelets is now possible by freezing with dimethylsulfoxide (DMSO). The platelets have a nearly normal survival upon reinfusion and are capable of sustained hemostatic effectiveness in thrombocytopenic patients. Adaptation of the freezing technique for large-scale usage has more recently been achieved. The method is mainly based on the following principles: (1) use of plasma for suspension of the platelet concentrate; (2) gradual addition (0.5% every 2 min) of DMSO to a final concentration of 5% and its gradual removal; (3) a slow cooling rate of about 1/sup 0/C per min and rapid thawing (in 1 min); (4) use of a polyolefin plastic bag for freezing; (5) a washing medium of 20% plasma in Hanks' balanced salt solution; (6) final resuspension of the platelets in 50% plasma in Hanks' solution.
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
EDB-80-024076
Resource Relation:
Journal Name: Vox Sang.; (Switzerland); Journal Volume: 30
Subject:
59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD PLATELETS; FREEZING; BLOOD PLASMA; DMSO; HEMIC DISEASES; HEMORRHAGE; INFUSION; MAN; POLYOLEFINS; PRESERVATION; STORAGE LIFE; THERAPY; VIABILITY; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; DISEASES; INTAKE; MAMMALS; ORGANIC COMPOUNDS; ORGANIC POLYMERS; ORGANIC SULFUR COMPOUNDS; PATHOLOGICAL CHANGES; POLYMERS; PRIMATES; SULFOXIDES; SYMPTOMS; VERTEBRATES; 550300* - Cytology; 550600 - Medicine
OSTI ID:
5679920
Research Organizations:
Brown Univ., Providence, RI
Country of Origin:
Switzerland
Language:
English
Contract Number:
AT(11-1)-3234
Other Identifying Numbers:
Journal ID: CODEN: VOSAA
Submitting Site:
TIC
Size:
Pages: 401-411
Announcement Date:

Journal Article:

Citation Formats

Kim, B K, Tanoue, K, and Baldini, M G. Storage of human platelets by freezing. Switzerland: N. p., 1976. Web.
Kim, B K, Tanoue, K, & Baldini, M G. Storage of human platelets by freezing. Switzerland.
Kim, B K, Tanoue, K, and Baldini, M G. 1976. "Storage of human platelets by freezing." Switzerland.
@misc{etde_5679920,
title = {Storage of human platelets by freezing}
author = {Kim, B K, Tanoue, K, and Baldini, M G}
abstractNote = {Prolonged, probably indefinite storage of viable and functional human platelets is now possible by freezing with dimethylsulfoxide (DMSO). The platelets have a nearly normal survival upon reinfusion and are capable of sustained hemostatic effectiveness in thrombocytopenic patients. Adaptation of the freezing technique for large-scale usage has more recently been achieved. The method is mainly based on the following principles: (1) use of plasma for suspension of the platelet concentrate; (2) gradual addition (0.5% every 2 min) of DMSO to a final concentration of 5% and its gradual removal; (3) a slow cooling rate of about 1/sup 0/C per min and rapid thawing (in 1 min); (4) use of a polyolefin plastic bag for freezing; (5) a washing medium of 20% plasma in Hanks' balanced salt solution; (6) final resuspension of the platelets in 50% plasma in Hanks' solution.}
journal = {Vox Sang.; (Switzerland)}
volume = {30}
journal type = {AC}
place = {Switzerland}
year = {1976}
month = {Jan}
}