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Determination of antithrombin III by radioimmunoassay and its clinical application

Journal Article:

Abstract

A radioimmunoassay (RIA) has been developed for the determination of antithrombin III (AT III) in man. The detection limit was 25 ..mu..g/dl. At III-RIA level and biological activity (anti-Xa) was significantly correlated (r = 0.737, P < 0.0001). Plasma levels in 36 healthy males (mean +- SD, 19.9 +- 2.5 mg/dl) and 21 healthy females (19.1 +- 2.4 mg/dl) were similiar. Serial AT III measurements in normal menstruating females showed lower levels during midcycle and higher concentrations during menstruation. In carcinomas, the AT III levels were lower than normal, particularly in hepatocellular carcinoma. In cirrhosis of liver, the levels were markedly decreased and in some patients were below that found in congenital AT III deficiency. Patients with deep vein thrombosis and patients with heart valve replacement had lower levels than normal, while patients with cerebral vascular occlusion had normal levels. The possible use of AT III as a diagnostic tool of post-operative deep vein thrombosis was demonstrated in one patient after hysterectomy. The increased sensitivity, specificity and precision of this type of assay offer distinct advantages over existing methods of AT III estimation.
Authors:
Chan, V; Chan, T K; Wong, V; Tso, S G; Todd, D [1] 
  1. Queen Mary Hospital, Hong Kong
Publication Date:
Apr 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-10-473411; EDB-80-024155
Resource Relation:
Journal Name: Br. J. Haematol.; (United Kingdom); Journal Volume: 41:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTICOAGULANTS; RADIOIMMUNOASSAY; THROMBOSIS; DIAGNOSTIC TECHNIQUES; ACCURACY; BLOOD PLASMA; BRAIN; CARCINOMAS; ENZYMES; ESTROUS CYCLE; EXPERIMENTAL DATA; GLOBULINS; GRAPHS; HEART; IODINE 125; LABELLED COMPOUNDS; LIVER CIRRHOSIS; PROSTHESES; SENSITIVITY; SURGERY; THROMBIN; VALVES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD COAGULATION FACTORS; BODY; BODY FLUIDS; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; CENTRAL NERVOUS SYSTEM; CONTROL EQUIPMENT; DATA; DATA FORMS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM DISEASES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EQUIPMENT; FLOW REGULATORS; HYDROLASES; INFORMATION; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MEDICAL SUPPLIES; MEDICINE; NEOPLASMS; NERVOUS SYSTEM; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HYDROLASES; PROTEINS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5679252
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BJHEA
Submitting Site:
INIS
Size:
Pages: 563-572
Announcement Date:
Aug 01, 1979

Journal Article:

Citation Formats

Chan, V, Chan, T K, Wong, V, Tso, S G, and Todd, D. Determination of antithrombin III by radioimmunoassay and its clinical application. United Kingdom: N. p., 1979. Web.
Chan, V, Chan, T K, Wong, V, Tso, S G, & Todd, D. Determination of antithrombin III by radioimmunoassay and its clinical application. United Kingdom.
Chan, V, Chan, T K, Wong, V, Tso, S G, and Todd, D. 1979. "Determination of antithrombin III by radioimmunoassay and its clinical application." United Kingdom.
@misc{etde_5679252,
title = {Determination of antithrombin III by radioimmunoassay and its clinical application}
author = {Chan, V, Chan, T K, Wong, V, Tso, S G, and Todd, D}
abstractNote = {A radioimmunoassay (RIA) has been developed for the determination of antithrombin III (AT III) in man. The detection limit was 25 ..mu..g/dl. At III-RIA level and biological activity (anti-Xa) was significantly correlated (r = 0.737, P < 0.0001). Plasma levels in 36 healthy males (mean +- SD, 19.9 +- 2.5 mg/dl) and 21 healthy females (19.1 +- 2.4 mg/dl) were similiar. Serial AT III measurements in normal menstruating females showed lower levels during midcycle and higher concentrations during menstruation. In carcinomas, the AT III levels were lower than normal, particularly in hepatocellular carcinoma. In cirrhosis of liver, the levels were markedly decreased and in some patients were below that found in congenital AT III deficiency. Patients with deep vein thrombosis and patients with heart valve replacement had lower levels than normal, while patients with cerebral vascular occlusion had normal levels. The possible use of AT III as a diagnostic tool of post-operative deep vein thrombosis was demonstrated in one patient after hysterectomy. The increased sensitivity, specificity and precision of this type of assay offer distinct advantages over existing methods of AT III estimation.}
journal = {Br. J. Haematol.; (United Kingdom)}
volume = {41:4}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Apr}
}