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Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to cell kinetics in patients with acute leukemia

Journal Article:

Abstract

Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to leukemic cell kinetics in five patients with acute myeloblastic leukemia (AML) were examined. Labeling efficiency of leukemic cells was 80.3 +- 3.6% for more than 1 x 10/sup 8/ cells at room temperature for 20 minutes of incubation followed by two times washes. Cell viability determined by means of trypanblue exclusion test was 95.3 +- 2.6%. In vitro elution rate of /sup 111/In from the labeled cells during 12 hours was 10.0 +- 1.2%. The disappearance curves of labeled leukemic cells in AMLs followed a single exponential fashion, and the half time of disappearance (T 1/2) ranged from 9.6 to 31.8 hours. Total blood leukemic cell pool (TBLCP) calculated with the dilution principles of radioisotopes correlated significantly with the leukemic cell counts (LC) in the peripheral blood (Y = 0.32 + 1.94X, r = 0.99). In the studies of organ distribution which were observed and analyzed with gamma camera and computer, labeled leukemic cells passed through lungs within 15 minutes. Radioactivity in the spleen increased rapidly for 30 - 60 minutes, then reached a plateau. Hepatic radioactivity showed a temporary decrease during 10 - 60 minutes following  More>>
Authors:
Takagi, Yuhkoh; Matsuda, Shin; Uchida, Tatsumi; Kariyone, Shigeo [1] 
  1. Fukushima Medical Coll. (Japan)
Publication Date:
Apr 01, 1984
Product Type:
Journal Article
Reference Number:
AIX-16-024139; EDB-85-097561
Resource Relation:
Journal Name: Kaku Igaku; (Japan); Journal Volume: 21:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; INDIUM 111; CELL CYCLE; LEUKOCYTES; LABELLING; BLOOD-PLASMA CLEARANCE; CELL CULTURES; DIAGNOSIS; DISTRIBUTION; IMAGES; INCUBATION; INDIUM OXIDES; MYELOID LEUKEMIA; OXINE; PATIENTS; VIABILITY; AROMATICS; AZAARENES; AZINES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHALCOGENIDES; CLEARANCE; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; HEMIC DISEASES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDIUM COMPOUNDS; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKEMIA; MATERIALS; MINUTES LIVING RADIOISOTOPES; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PYRIDINES; QUINOLINES; RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5665300
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: KAIGB
Submitting Site:
HEDB
Size:
Pages: 341-351
Announcement Date:

Journal Article:

Citation Formats

Takagi, Yuhkoh, Matsuda, Shin, Uchida, Tatsumi, and Kariyone, Shigeo. Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to cell kinetics in patients with acute leukemia. Japan: N. p., 1984. Web.
Takagi, Yuhkoh, Matsuda, Shin, Uchida, Tatsumi, & Kariyone, Shigeo. Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to cell kinetics in patients with acute leukemia. Japan.
Takagi, Yuhkoh, Matsuda, Shin, Uchida, Tatsumi, and Kariyone, Shigeo. 1984. "Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to cell kinetics in patients with acute leukemia." Japan.
@misc{etde_5665300,
title = {Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to cell kinetics in patients with acute leukemia}
author = {Takagi, Yuhkoh, Matsuda, Shin, Uchida, Tatsumi, and Kariyone, Shigeo}
abstractNote = {Fundamental studies of leukemic cell labeling with /sup 111/In-oxine and their applications to leukemic cell kinetics in five patients with acute myeloblastic leukemia (AML) were examined. Labeling efficiency of leukemic cells was 80.3 +- 3.6% for more than 1 x 10/sup 8/ cells at room temperature for 20 minutes of incubation followed by two times washes. Cell viability determined by means of trypanblue exclusion test was 95.3 +- 2.6%. In vitro elution rate of /sup 111/In from the labeled cells during 12 hours was 10.0 +- 1.2%. The disappearance curves of labeled leukemic cells in AMLs followed a single exponential fashion, and the half time of disappearance (T 1/2) ranged from 9.6 to 31.8 hours. Total blood leukemic cell pool (TBLCP) calculated with the dilution principles of radioisotopes correlated significantly with the leukemic cell counts (LC) in the peripheral blood (Y = 0.32 + 1.94X, r = 0.99). In the studies of organ distribution which were observed and analyzed with gamma camera and computer, labeled leukemic cells passed through lungs within 15 minutes. Radioactivity in the spleen increased rapidly for 30 - 60 minutes, then reached a plateau. Hepatic radioactivity showed a temporary decrease during 10 - 60 minutes following the moderate accumulation in initial 10 minutes. In two cases, bone marrow was visualized 24 hours after the injection. Radioactivity of the leukemic cells isolated from the bone marrow at 22 hours after the injection in one case was one third of the radioactivity in leukemic cells obtained from the peripheral blood at the same time.}
journal = {Kaku Igaku; (Japan)}
volume = {21:4}
journal type = {AC}
place = {Japan}
year = {1984}
month = {Apr}
}