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Ethanol fermentation with a flocculating yeast

Abstract

A 100 cm x 5.7 cm internal diameter tower fermentor was fabricated and operated continuously for 11 months using the floc-forming yeast, Saccharomyces cerevisiae (American Type Culture Collection 4097). Steady state operation of the system was characterized at 32/sup 0/C and pH 4.0 for glucose concentrations ranging from 105 to 215 g l/sup -1/. The height of the yeast bed in the tower was maintained at 80 cm. The high yeast density, ethanol concentration and low pH prevented bacterial contamination in the reactor. The concentration profiles of glucose and ethanol within the bed were described by a dispersion model. Modeling parameters were determined for the yeast by batch kinetics and tracer experiments. The kinetic model included ethanol inhibition and substrate limitation. A tracer study with step input of D-xylose (a non-metabolizable sugar for S. cerevisiae) determined the dispersion number (D/uL=0.16) and liquid voidage (epsilonsub(L)=0.25). Measurements taken after 6 months of continuous operation indicated that there was no significant change in fermentor performance.
Publication Date:
Aug 01, 1985
Product Type:
Journal Article
Reference Number:
DE-86-006929; EDB-86-115927
Resource Relation:
Journal Name: Chem. Eng. J. (Lausanne); (Switzerland); Journal Volume: 31:1
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ETHANOL; BIOSYNTHESIS; SACCHAROMYCES CEREVISIAE; FLOCCULATION; BATCH CULTURE; BIOCHEMICAL REACTION KINETICS; BIOREACTORS; CULTURE MEDIA; FERMENTATION; GLUCOSE; MATHEMATICAL MODELS; MESOPHILIC CONDITIONS; PERFORMANCE; PH VALUE; PRODUCTIVITY; XYLOSE; ALCOHOLS; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; FUNGI; HEXOSES; HYDROXY COMPOUNDS; KINETICS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; PLANTS; PRECIPITATION; REACTION KINETICS; SACCHARIDES; SACCHAROMYCES; SEPARATION PROCESSES; SYNTHESIS; YEASTS; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 550200 - Biochemistry; 550700 - Microbiology
OSTI ID:
5663572
Research Organizations:
Idaho Univ., Moscow, USA. Dept. of Chemical Engineering; Idaho Univ., Moscow, USA. Dept. of Bacteriology and Biochemistry
Country of Origin:
Switzerland
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CMEJA
Submitting Site:
DE
Size:
Pages: B1-B8
Announcement Date:

Citation Formats

Admassu, W, Korus, R A, and Heimsch, R C. Ethanol fermentation with a flocculating yeast. Switzerland: N. p., 1985. Web. doi:10.1016/0300-9467(85)85014-0.
Admassu, W, Korus, R A, & Heimsch, R C. Ethanol fermentation with a flocculating yeast. Switzerland. doi:10.1016/0300-9467(85)85014-0.
Admassu, W, Korus, R A, and Heimsch, R C. 1985. "Ethanol fermentation with a flocculating yeast." Switzerland. doi:10.1016/0300-9467(85)85014-0. https://www.osti.gov/servlets/purl/10.1016/0300-9467(85)85014-0.
@misc{etde_5663572,
title = {Ethanol fermentation with a flocculating yeast}
author = {Admassu, W, Korus, R A, and Heimsch, R C}
abstractNote = {A 100 cm x 5.7 cm internal diameter tower fermentor was fabricated and operated continuously for 11 months using the floc-forming yeast, Saccharomyces cerevisiae (American Type Culture Collection 4097). Steady state operation of the system was characterized at 32/sup 0/C and pH 4.0 for glucose concentrations ranging from 105 to 215 g l/sup -1/. The height of the yeast bed in the tower was maintained at 80 cm. The high yeast density, ethanol concentration and low pH prevented bacterial contamination in the reactor. The concentration profiles of glucose and ethanol within the bed were described by a dispersion model. Modeling parameters were determined for the yeast by batch kinetics and tracer experiments. The kinetic model included ethanol inhibition and substrate limitation. A tracer study with step input of D-xylose (a non-metabolizable sugar for S. cerevisiae) determined the dispersion number (D/uL=0.16) and liquid voidage (epsilonsub(L)=0.25). Measurements taken after 6 months of continuous operation indicated that there was no significant change in fermentor performance.}
doi = {10.1016/0300-9467(85)85014-0}
journal = {Chem. Eng. J. (Lausanne); (Switzerland)}
volume = {31:1}
journal type = {AC}
place = {Switzerland}
year = {1985}
month = {Aug}
}