Abstract
Iodination of insulin using N-dichloro-p-toluene sulphonamide(dichloramine-T) has been standardised. Dichloramine-T, a water insoluble derivative of chloramine-T showed excellent properties as an iodination reagent, for the preparation of radiolabeled insulin for use in radioimmunoassay. Iodination using dichloramine-T could be done at as low as 0.5-2 ..mu..g of the reagent, and at this concentration the molar ratio worked out to 1:3 to 1:12 (protein: dichloramine-T). Iodination yields of greater than 90% were obtained at pH 5-7. Evaluation of the iodinated tracer for suitability in radioimmunoassay was carried out by estimating radiochemical purity, immunological purity, non specific binding and stability on storage.
Citation Formats
Jyotsna, T, Pillai, M R.A., Gupte, J H, and Mani, R S.
Mild radioiodination of insulin with dichloramine-T.
United Kingdom: N. p.,
1986.
Web.
Jyotsna, T, Pillai, M R.A., Gupte, J H, & Mani, R S.
Mild radioiodination of insulin with dichloramine-T.
United Kingdom.
Jyotsna, T, Pillai, M R.A., Gupte, J H, and Mani, R S.
1986.
"Mild radioiodination of insulin with dichloramine-T."
United Kingdom.
@misc{etde_5659006,
title = {Mild radioiodination of insulin with dichloramine-T}
author = {Jyotsna, T, Pillai, M R.A., Gupte, J H, and Mani, R S}
abstractNote = {Iodination of insulin using N-dichloro-p-toluene sulphonamide(dichloramine-T) has been standardised. Dichloramine-T, a water insoluble derivative of chloramine-T showed excellent properties as an iodination reagent, for the preparation of radiolabeled insulin for use in radioimmunoassay. Iodination using dichloramine-T could be done at as low as 0.5-2 ..mu..g of the reagent, and at this concentration the molar ratio worked out to 1:3 to 1:12 (protein: dichloramine-T). Iodination yields of greater than 90% were obtained at pH 5-7. Evaluation of the iodinated tracer for suitability in radioimmunoassay was carried out by estimating radiochemical purity, immunological purity, non specific binding and stability on storage.}
journal = []
volume = {13:5}
journal type = {AC}
place = {United Kingdom}
year = {1986}
month = {Jan}
}
title = {Mild radioiodination of insulin with dichloramine-T}
author = {Jyotsna, T, Pillai, M R.A., Gupte, J H, and Mani, R S}
abstractNote = {Iodination of insulin using N-dichloro-p-toluene sulphonamide(dichloramine-T) has been standardised. Dichloramine-T, a water insoluble derivative of chloramine-T showed excellent properties as an iodination reagent, for the preparation of radiolabeled insulin for use in radioimmunoassay. Iodination using dichloramine-T could be done at as low as 0.5-2 ..mu..g of the reagent, and at this concentration the molar ratio worked out to 1:3 to 1:12 (protein: dichloramine-T). Iodination yields of greater than 90% were obtained at pH 5-7. Evaluation of the iodinated tracer for suitability in radioimmunoassay was carried out by estimating radiochemical purity, immunological purity, non specific binding and stability on storage.}
journal = []
volume = {13:5}
journal type = {AC}
place = {United Kingdom}
year = {1986}
month = {Jan}
}