You need JavaScript to view this

Primary assimilation process of triply (/sup 15/N, /sup 14/C and /sup 3/H) labeled arginine in the roots of arginine-fed barley

Abstract

To clarify the mechanism of arginine utilization in barley roots, triply labeled (ureido-/sup 15/N, ureido-/sup 14/C and 5-/sup 3/H) arginine was applied to plants precultured with arginine (Arg-plants). (5-/sup 3/H) Arginine was incorporated mainly into ornithine, suggesting that arginase contributes in the first step of arginine metabolism. The arginase activity in the tissues was greatly enhanced by continuous supply of arginine, whereas urease activity was not by the same treatment. The amount of /sup 14/CO/sub 2/ evolved from (ureido-/sup 14/C) arginine in the Arg-plants was several times higher than that in plants treated with NO/sub 3//sup -/(NO/sub 3/-plants), and most /sup 14/C-urea exogenously supplied to detached roots of Arg-plants was immediately decomposed to /sup 14/CO/sub 2/. The urea released from arginine by arginase was cleaved to /sup 15/NH/sub 4//sup +/ + /sup 14/CO/sub 2/ by urease. Most of the /sup 14/CO/sub 2/ was then lost from the root system. On the other hand, the released /sup 15/NH/sub 4//sup +/ was reassimilated into amino acids probably through the pathway of ammonia assimilation. Released (5-/sup 3/H) ornithine was metabolized dominantly to proline.
Authors:
Mori, Satoshi [1] 
  1. Tokyo Univ. (Japan). Faculty of Agriculture
Publication Date:
Mar 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-13-668734; EDB-82-090383
Resource Relation:
Journal Name: Soil Sci. Plant Nutr. (Tokyo); (Japan); Journal Volume: 27:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; ARGININE; METABOLISM; ROOT ABSORPTION; CARBON 14 COMPOUNDS; TRACER TECHNIQUES; NITROGEN 15; TRITIUM COMPOUNDS; BARLEY; LABELLED COMPOUNDS; LABELLING; ROOTS; ABSORPTION; AMINO ACIDS; CARBOXYLIC ACIDS; CEREALS; GRAMINEAE; GRASS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NITROGEN ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; STABLE ISOTOPES; UPTAKE; 550501* - Metabolism- Tracer Techniques
OSTI ID:
5655703
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: SSPNA
Submitting Site:
HEDB
Size:
Pages: 29-43
Announcement Date:
Apr 01, 1982

Citation Formats

Mori, Satoshi. Primary assimilation process of triply (/sup 15/N, /sup 14/C and /sup 3/H) labeled arginine in the roots of arginine-fed barley. Japan: N. p., 1981. Web.
Mori, Satoshi. Primary assimilation process of triply (/sup 15/N, /sup 14/C and /sup 3/H) labeled arginine in the roots of arginine-fed barley. Japan.
Mori, Satoshi. 1981. "Primary assimilation process of triply (/sup 15/N, /sup 14/C and /sup 3/H) labeled arginine in the roots of arginine-fed barley." Japan.
@misc{etde_5655703,
title = {Primary assimilation process of triply (/sup 15/N, /sup 14/C and /sup 3/H) labeled arginine in the roots of arginine-fed barley}
author = {Mori, Satoshi}
abstractNote = {To clarify the mechanism of arginine utilization in barley roots, triply labeled (ureido-/sup 15/N, ureido-/sup 14/C and 5-/sup 3/H) arginine was applied to plants precultured with arginine (Arg-plants). (5-/sup 3/H) Arginine was incorporated mainly into ornithine, suggesting that arginase contributes in the first step of arginine metabolism. The arginase activity in the tissues was greatly enhanced by continuous supply of arginine, whereas urease activity was not by the same treatment. The amount of /sup 14/CO/sub 2/ evolved from (ureido-/sup 14/C) arginine in the Arg-plants was several times higher than that in plants treated with NO/sub 3//sup -/(NO/sub 3/-plants), and most /sup 14/C-urea exogenously supplied to detached roots of Arg-plants was immediately decomposed to /sup 14/CO/sub 2/. The urea released from arginine by arginase was cleaved to /sup 15/NH/sub 4//sup +/ + /sup 14/CO/sub 2/ by urease. Most of the /sup 14/CO/sub 2/ was then lost from the root system. On the other hand, the released /sup 15/NH/sub 4//sup +/ was reassimilated into amino acids probably through the pathway of ammonia assimilation. Released (5-/sup 3/H) ornithine was metabolized dominantly to proline.}
journal = []
volume = {27:1}
journal type = {AC}
place = {Japan}
year = {1981}
month = {Mar}
}