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Fermentation of sugar to ethyl alcohol in the presence of proteolytic enzymes

Abstract

Sugar is fermented to EtOH by yeasts capable of elaborating zymase and proteolytic enzymes, the zymase component comprising exceptionally large amounts of phosphatase. Saccharomyces ellipsoideus was acclimated to 20% EtOH by growing on fresh pineapple juice in a medium consisting of malt sirup 15, sugar sirup 3, and pineapple juice 82%. An aqueous solution of 2000 gallons of sugar cane molasses in H/sub 2/O to give a Brix of 16/sup 0/ was placed in a 48,000-gallon fermentor. S. ellipsoideus with a cell constant of 1 x 10/sup 9/ cells/ml was added, with sufficient H/sub 2/SO/sub 4/ to adjust the pH to approximately 4.5. Fermentation was carried out at 35/sup 0/ until the Brix dropped to 8/sup 0/, after which it was brought back to 16/sup 0/ by adding 6000 gallons of sirup containing nutrients in H/sub 2/O. This process was repeated with another 6000 and then 2000 gallons of sirup. The total fermentation required 48 h and the EtOH content was 15.25% by volume. Te EtOH was recovered in the usual manner by removal of solids and fractional distillation.
Publication Date:
Jun 11, 1963
Product Type:
Patent
Report Number:
US 3093548
Reference Number:
EDB-80-039400
Resource Relation:
Patent File Date: Filed date 3 Aug 1961
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; ETHANOL; BIOSYNTHESIS; SACCHAROSE; FERMENTATION; ENZYMES; MALTOSE; MOLASSES; PHOSPHATASES; SULFURIC ACID; YEASTS; ALCOHOLS; BIOCONVERSION; CARBOHYDRATES; DISACCHARIDES; ESTERASES; FUNGI; HYDROGEN COMPOUNDS; HYDROLASES; HYDROXY COMPOUNDS; INORGANIC ACIDS; MICROORGANISMS; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; SACCHARIDES; SYNTHESIS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550700 - Microbiology
OSTI ID:
5649509
Country of Origin:
United States
Language:
English
Submitting Site:
IEA
Size:
Pages: 4
Announcement Date:
May 13, 2001

Citation Formats

Coates, E W, and Conde Julio, C. Fermentation of sugar to ethyl alcohol in the presence of proteolytic enzymes. United States: N. p., 1963. Web.
Coates, E W, & Conde Julio, C. Fermentation of sugar to ethyl alcohol in the presence of proteolytic enzymes. United States.
Coates, E W, and Conde Julio, C. 1963. "Fermentation of sugar to ethyl alcohol in the presence of proteolytic enzymes." United States.
@misc{etde_5649509,
title = {Fermentation of sugar to ethyl alcohol in the presence of proteolytic enzymes}
author = {Coates, E W, and Conde Julio, C}
abstractNote = {Sugar is fermented to EtOH by yeasts capable of elaborating zymase and proteolytic enzymes, the zymase component comprising exceptionally large amounts of phosphatase. Saccharomyces ellipsoideus was acclimated to 20% EtOH by growing on fresh pineapple juice in a medium consisting of malt sirup 15, sugar sirup 3, and pineapple juice 82%. An aqueous solution of 2000 gallons of sugar cane molasses in H/sub 2/O to give a Brix of 16/sup 0/ was placed in a 48,000-gallon fermentor. S. ellipsoideus with a cell constant of 1 x 10/sup 9/ cells/ml was added, with sufficient H/sub 2/SO/sub 4/ to adjust the pH to approximately 4.5. Fermentation was carried out at 35/sup 0/ until the Brix dropped to 8/sup 0/, after which it was brought back to 16/sup 0/ by adding 6000 gallons of sirup containing nutrients in H/sub 2/O. This process was repeated with another 6000 and then 2000 gallons of sirup. The total fermentation required 48 h and the EtOH content was 15.25% by volume. Te EtOH was recovered in the usual manner by removal of solids and fractional distillation.}
place = {United States}
year = {1963}
month = {Jun}
}