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Effects of irradiation on cytokine production in glioma cell lines

Journal Article:

Abstract

The effects of irradiation on cytokine production in glioma cell lines, NP1, NP2 and NP3, were studied. Culture supernatants were collected after 6, 24, 48 or 72 hours and the concentrations of interleukin (IL)-6 and IL-8 measured by enzyme-linked immunosorbent assay. Spontaneous and IL-1[beta]-stimulated productions were analyzed. Some cells were given a single dose of Lineac irradiation (10 or 20 Gy). Production of IL-6 (with or without IL-1[beta] stimulation) increased gradually to a maximum after 72 hours, more in the 20 Gy-irradiated cells than 10 Gy cells (p<0.01). Production of IL-8 increased gradually to a maximum after 48 or 72 hours. Spontaneous production of IL-8 increased more in 20 Gy-irradiated cells than 10 Gy cells after 6 and 24 hours (p<0.01), but increased more in 10 Gy cells than 20 Gy cells after 48 and 72 hours (p<0.01). The production of IL-8 stimulated by IL-1[beta] increased more in 10 Gy cells than 20 Gy cells 24 hours later (p<0.01). IL-6 and IL-8 production differed in the response to irradiation. Our data suggest that bidirectional communication between the immune system and glioma cells changes after radiotherapy. (author).
Authors:
Yamanaka, Ryuya; Tanaka, Ryuichi; Yoshida, Seiichi [1] 
  1. Niigata Univ. (Japan). Brain Research Inst.
Publication Date:
Nov 01, 1993
Product Type:
Journal Article
Reference Number:
JPN-93-012272; EDB-94-027166
Resource Relation:
Journal Name: Neurologia Medico-Chirurgica; (Japan); Journal Volume: 33:11
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; COMPLEMENT; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; CULTURE MEDIA; GLIOMAS; IMMUNITY; INCUBATION; RADIATION DOSES; RADIOTHERAPY; TUMOR CELLS; ANIMAL CELLS; BIOLOGICAL EFFECTS; DISEASES; DOSES; MEDICINE; NEOPLASMS; NUCLEAR MEDICINE; ORGANIC COMPOUNDS; PROTEINS; RADIATION EFFECTS; RADIOLOGY; THERAPY; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
5645676
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0387-2572; CODEN: NMCHBN
Submitting Site:
JPN
Size:
Pages: 744-748
Announcement Date:

Journal Article:

Citation Formats

Yamanaka, Ryuya, Tanaka, Ryuichi, and Yoshida, Seiichi. Effects of irradiation on cytokine production in glioma cell lines. Japan: N. p., 1993. Web.
Yamanaka, Ryuya, Tanaka, Ryuichi, & Yoshida, Seiichi. Effects of irradiation on cytokine production in glioma cell lines. Japan.
Yamanaka, Ryuya, Tanaka, Ryuichi, and Yoshida, Seiichi. 1993. "Effects of irradiation on cytokine production in glioma cell lines." Japan.
@misc{etde_5645676,
title = {Effects of irradiation on cytokine production in glioma cell lines}
author = {Yamanaka, Ryuya, Tanaka, Ryuichi, and Yoshida, Seiichi}
abstractNote = {The effects of irradiation on cytokine production in glioma cell lines, NP1, NP2 and NP3, were studied. Culture supernatants were collected after 6, 24, 48 or 72 hours and the concentrations of interleukin (IL)-6 and IL-8 measured by enzyme-linked immunosorbent assay. Spontaneous and IL-1[beta]-stimulated productions were analyzed. Some cells were given a single dose of Lineac irradiation (10 or 20 Gy). Production of IL-6 (with or without IL-1[beta] stimulation) increased gradually to a maximum after 72 hours, more in the 20 Gy-irradiated cells than 10 Gy cells (p<0.01). Production of IL-8 increased gradually to a maximum after 48 or 72 hours. Spontaneous production of IL-8 increased more in 20 Gy-irradiated cells than 10 Gy cells after 6 and 24 hours (p<0.01), but increased more in 10 Gy cells than 20 Gy cells after 48 and 72 hours (p<0.01). The production of IL-8 stimulated by IL-1[beta] increased more in 10 Gy cells than 20 Gy cells 24 hours later (p<0.01). IL-6 and IL-8 production differed in the response to irradiation. Our data suggest that bidirectional communication between the immune system and glioma cells changes after radiotherapy. (author).}
journal = {Neurologia Medico-Chirurgica; (Japan)}
volume = {33:11}
journal type = {AC}
place = {Japan}
year = {1993}
month = {Nov}
}