Abstract
The effect of Ro 7-0582 (1-(2-hydroxy-3-methoxypropyl)-2-nitro-imidazole) on the formation of free radicals in ..gamma..-irradiated dry DNA has been investigated. Dry samples of DNA-Ro 7-0582 and DNA nucleotide-Ro 7-0582 were prepared, and e.s.r. spectra observed at 77 K immediately after gamma-irradiation. The samples were then warmed to 297 K for 30 min, and the spectra again observed at 77 K. The sensitizer brought about an increase of 30 to 40% in radical formation in DNA. The results indicated that Ro 7-0582 acts as an efficient electron scavenger on the TMP and dAMP moieties, increasing the incidence of sugar damage. Since TMP and dAMP form a complementary pair in the DNA double helix, the increase in double strand breaks induced by electron-affinic compounds seems to be responsible for the molecular mechanism of radiosensitization in living cells.
Citation Formats
Washino, K, Kuwabara, M, and Yoshii, G.
E. S. R. study of free radicals formed in the irradiated DNA-Ro 7-0582 complex.
United Kingdom: N. p.,
1979.
Web.
doi:10.1080/09553007914550081.
Washino, K, Kuwabara, M, & Yoshii, G.
E. S. R. study of free radicals formed in the irradiated DNA-Ro 7-0582 complex.
United Kingdom.
https://doi.org/10.1080/09553007914550081
Washino, K, Kuwabara, M, and Yoshii, G.
1979.
"E. S. R. study of free radicals formed in the irradiated DNA-Ro 7-0582 complex."
United Kingdom.
https://doi.org/10.1080/09553007914550081.
@misc{etde_5638971,
title = {E. S. R. study of free radicals formed in the irradiated DNA-Ro 7-0582 complex}
author = {Washino, K, Kuwabara, M, and Yoshii, G}
abstractNote = {The effect of Ro 7-0582 (1-(2-hydroxy-3-methoxypropyl)-2-nitro-imidazole) on the formation of free radicals in ..gamma..-irradiated dry DNA has been investigated. Dry samples of DNA-Ro 7-0582 and DNA nucleotide-Ro 7-0582 were prepared, and e.s.r. spectra observed at 77 K immediately after gamma-irradiation. The samples were then warmed to 297 K for 30 min, and the spectra again observed at 77 K. The sensitizer brought about an increase of 30 to 40% in radical formation in DNA. The results indicated that Ro 7-0582 acts as an efficient electron scavenger on the TMP and dAMP moieties, increasing the incidence of sugar damage. Since TMP and dAMP form a complementary pair in the DNA double helix, the increase in double strand breaks induced by electron-affinic compounds seems to be responsible for the molecular mechanism of radiosensitization in living cells.}
doi = {10.1080/09553007914550081}
journal = []
volume = {35:1}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}
title = {E. S. R. study of free radicals formed in the irradiated DNA-Ro 7-0582 complex}
author = {Washino, K, Kuwabara, M, and Yoshii, G}
abstractNote = {The effect of Ro 7-0582 (1-(2-hydroxy-3-methoxypropyl)-2-nitro-imidazole) on the formation of free radicals in ..gamma..-irradiated dry DNA has been investigated. Dry samples of DNA-Ro 7-0582 and DNA nucleotide-Ro 7-0582 were prepared, and e.s.r. spectra observed at 77 K immediately after gamma-irradiation. The samples were then warmed to 297 K for 30 min, and the spectra again observed at 77 K. The sensitizer brought about an increase of 30 to 40% in radical formation in DNA. The results indicated that Ro 7-0582 acts as an efficient electron scavenger on the TMP and dAMP moieties, increasing the incidence of sugar damage. Since TMP and dAMP form a complementary pair in the DNA double helix, the increase in double strand breaks induced by electron-affinic compounds seems to be responsible for the molecular mechanism of radiosensitization in living cells.}
doi = {10.1080/09553007914550081}
journal = []
volume = {35:1}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}