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Radioimmunoassay of inactive creatine kinase B protein in human plasma

Abstract

The authors describe a rapid, sensitive radioimmunoassay for enzymatically inactive creatine kinase B protein (CK-Bi) in plasma. /sup 125/I-CK-Bi of high specific activity and good stability was prepared by oxidant-based iodination. A 12-minute first antibody incubation was used. Bound and free antigen were separated by a second antibody system. Large excesses of purified CK-MM from human skeletal muscle did not react in the assay. Cross reactivity to CK-MB purified from the plasma of patients with acute myocardial infarction was negligible. The 95th percentile of plasma CK-Bi in 150 adults was 145 ..mu..g equivalents/ml. Within-assay and between-assay precision ranged from 5% to 9% and 6% to 10%, respectively. Evidence is presented indicating that the assay measures inactive creatine kinase B protein, a protein not measured by current assay systems dependent on biological activity.
Authors:
Burnam, M H; Shell, W E [1] 
  1. California Univ., Los Angeles (USA). School of Medicine
Publication Date:
Aug 27, 1981
Product Type:
Journal Article
Reference Number:
AIX-13-647877; EDB-82-050571
Resource Relation:
Journal Name: Clin. Chim. Acta; (Netherlands); Journal Volume: 115:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CREATINE; PHOSPHOTRANSFERASES; RADIOIMMUNOASSAY; ACCURACY; ANTIBODIES; BIOCHEMICAL REACTION KINETICS; BLOOD PLASMA; IODINATION; IODINE 125; MYOCARDIAL INFARCTION; PROTEINS; QUANTITY RATIO; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CARBOXYLIC ACIDS; CARDIOVASCULAR DISEASES; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HALOGENATION; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; RADIOASSAY; RADIOISOTOPES; REACTION KINETICS; TRACER TECHNIQUES; TRANSFERASES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5631656
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CCATA
Submitting Site:
INIS
Size:
Pages: 33-46
Announcement Date:
Jan 01, 1982

Citation Formats

Burnam, M H, and Shell, W E. Radioimmunoassay of inactive creatine kinase B protein in human plasma. Netherlands: N. p., 1981. Web. doi:10.1016/0009-8981(81)90104-2.
Burnam, M H, & Shell, W E. Radioimmunoassay of inactive creatine kinase B protein in human plasma. Netherlands. doi:10.1016/0009-8981(81)90104-2.
Burnam, M H, and Shell, W E. 1981. "Radioimmunoassay of inactive creatine kinase B protein in human plasma." Netherlands. doi:10.1016/0009-8981(81)90104-2. https://www.osti.gov/servlets/purl/10.1016/0009-8981(81)90104-2.
@misc{etde_5631656,
title = {Radioimmunoassay of inactive creatine kinase B protein in human plasma}
author = {Burnam, M H, and Shell, W E}
abstractNote = {The authors describe a rapid, sensitive radioimmunoassay for enzymatically inactive creatine kinase B protein (CK-Bi) in plasma. /sup 125/I-CK-Bi of high specific activity and good stability was prepared by oxidant-based iodination. A 12-minute first antibody incubation was used. Bound and free antigen were separated by a second antibody system. Large excesses of purified CK-MM from human skeletal muscle did not react in the assay. Cross reactivity to CK-MB purified from the plasma of patients with acute myocardial infarction was negligible. The 95th percentile of plasma CK-Bi in 150 adults was 145 ..mu..g equivalents/ml. Within-assay and between-assay precision ranged from 5% to 9% and 6% to 10%, respectively. Evidence is presented indicating that the assay measures inactive creatine kinase B protein, a protein not measured by current assay systems dependent on biological activity.}
doi = {10.1016/0009-8981(81)90104-2}
journal = {Clin. Chim. Acta; (Netherlands)}
volume = {115:1}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Aug}
}