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Properties of murine leukemia viruses produced by leukemic cells established from NIH Swiss mice with radiation-induced leukemia

Journal Article:

Abstract

Three leukemic cell lines, designated NIH-RL1, NIH-RL2 and NFS-RL1, were established from spleen and thymuses of NIH Swiss and NFS mice with radiation-induced leukemia. The culture fluids of these cell lines contained RNA-dependent DNA polymerase (RDDP) activities associated with particles of buoyant density of 1.15-1.17 (g/cm/sup 3/). The divalent cation reqirement of these enzymes was characteristic for that of murine leukemia viruses. In competition radioimmunoassay, a major core protein, p30, was detected in culture fluid of each leukemic cell line. Competition curves of viral p30 produced by these cell lines revealed that these viruses were very similar to those of xenotropic viruses of NZB mice. These viruses were undetectable both by XC plaque assay using SC-1 cells as an indicator cell, and by mink S/sup +/L/sup -/ focus induction assay. These viruses also lacked productive infectivity to mink lung cells (CCL-64), and were nononcogenic in syngeneic mice when the viruses were intrathymically inoculated.
Authors:
Okumoto, Masaaki; Nishikawa, Ryosuke; Takamori, Yasuhiko; Iwai, Yoshiaki; Iwai, Mineko; [1]  Imai, Shunsuke; Morimoto, Junji; Tsubura, Yoshihiko
  1. Radiation Center of Osaka Prefecture, Sakai (Japan)
Publication Date:
Jun 01, 1984
Product Type:
Journal Article
Reference Number:
AIX-16-053802; EDB-85-116973
Resource Relation:
Journal Name: Nara Igaku Zasshi; (Japan); Journal Volume: 35:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LEUKEMIA VIRUSES; BIOLOGICAL RADIATION EFFECTS; X RADIATION; LEUKEMOGENESIS; CELL CULTURES; EXPERIMENTAL NEOPLASMS; IODINE 125; MICE; PLAQUE FORMATION; POLYMERASES; RADIOIMMUNOASSAY; RNA; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CARCINOGENESIS; DAYS LIVING RADIOISOTOPES; ELECTROMAGNETIC RADIATION; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; IONIZING RADIATIONS; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ODD-EVEN NUCLEI; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; PARASITES; PATHOGENESIS; PHOSPHORUS-GROUP TRANSFERASES; RADIATION EFFECTS; RADIATIONS; RADIOASSAY; RADIOISOTOPES; RODENTS; TRACER TECHNIQUES; TRANSFERASES; VERTEBRATES; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
5608783
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: NAIZA
Submitting Site:
HEDB
Size:
Pages: 334-342
Announcement Date:

Journal Article:

Citation Formats

Okumoto, Masaaki, Nishikawa, Ryosuke, Takamori, Yasuhiko, Iwai, Yoshiaki, Iwai, Mineko, Imai, Shunsuke, Morimoto, Junji, and Tsubura, Yoshihiko. Properties of murine leukemia viruses produced by leukemic cells established from NIH Swiss mice with radiation-induced leukemia. Japan: N. p., 1984. Web.
Okumoto, Masaaki, Nishikawa, Ryosuke, Takamori, Yasuhiko, Iwai, Yoshiaki, Iwai, Mineko, Imai, Shunsuke, Morimoto, Junji, & Tsubura, Yoshihiko. Properties of murine leukemia viruses produced by leukemic cells established from NIH Swiss mice with radiation-induced leukemia. Japan.
Okumoto, Masaaki, Nishikawa, Ryosuke, Takamori, Yasuhiko, Iwai, Yoshiaki, Iwai, Mineko, Imai, Shunsuke, Morimoto, Junji, and Tsubura, Yoshihiko. 1984. "Properties of murine leukemia viruses produced by leukemic cells established from NIH Swiss mice with radiation-induced leukemia." Japan.
@misc{etde_5608783,
title = {Properties of murine leukemia viruses produced by leukemic cells established from NIH Swiss mice with radiation-induced leukemia}
author = {Okumoto, Masaaki, Nishikawa, Ryosuke, Takamori, Yasuhiko, Iwai, Yoshiaki, Iwai, Mineko, Imai, Shunsuke, Morimoto, Junji, and Tsubura, Yoshihiko}
abstractNote = {Three leukemic cell lines, designated NIH-RL1, NIH-RL2 and NFS-RL1, were established from spleen and thymuses of NIH Swiss and NFS mice with radiation-induced leukemia. The culture fluids of these cell lines contained RNA-dependent DNA polymerase (RDDP) activities associated with particles of buoyant density of 1.15-1.17 (g/cm/sup 3/). The divalent cation reqirement of these enzymes was characteristic for that of murine leukemia viruses. In competition radioimmunoassay, a major core protein, p30, was detected in culture fluid of each leukemic cell line. Competition curves of viral p30 produced by these cell lines revealed that these viruses were very similar to those of xenotropic viruses of NZB mice. These viruses were undetectable both by XC plaque assay using SC-1 cells as an indicator cell, and by mink S/sup +/L/sup -/ focus induction assay. These viruses also lacked productive infectivity to mink lung cells (CCL-64), and were nononcogenic in syngeneic mice when the viruses were intrathymically inoculated.}
journal = {Nara Igaku Zasshi; (Japan)}
volume = {35:3}
journal type = {AC}
place = {Japan}
year = {1984}
month = {Jun}
}