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Studies on quantitative physiology of Trichoderma reesei with two-stage continuous culture for cellulase production

Abstract

By employing a two-stage continuous-culture system, some of the more important physiological parameters involved in cellulase biosynthesis have been evaluated with an ultimate objective of designing an optimally controlled cellulase process. The two-stage continuous-culture system was run for a period of 1350 hr with Trichoderma reesei strain MCG-77. The temperature and pH were controlled at 32/sup 0/C and pH 4.5 for the first stage (growth) and 28/sup 0/C and pH 3.5 for the second stage (enzyme production). Lactose was the only carbon source for both stages. The ratio of specific uptake rate of carbon to that of nitrogen, Q(C)/Q(N), that supported good cell growth ranged from 11 to 15, and the ratio for maximum specific enzyme productivity ranged from 5 to 13. The maintenance coefficients determined for oxygen, M/sub 0/, and for carbon source, M/sub c/, are 0.85 mmol O/sub 2//g biomass/hr and 0.14 mmol hexose/g biomass/hr, respectively. The yield constants determined are: Y/sub X/O/ = 32.3 g biomass/mol O/sub 2/, Y/sub X/C/ = 1.1 g biomass/g C or Y/sub X/C/ = 0.44 g biomass/g hexose, Y/sub X/N/ = 12.5 g biomass/g nitrogen for the cell growth stage, and Y/sub X/N/ = 16.6 g biomass/g nitrogen for the enzyme production  More>>
Publication Date:
Nov 01, 1979
Product Type:
Journal Article
Reference Number:
ERA-05-009312; EDB-80-018174
Resource Relation:
Journal Name: Biotechnol. Bioeng.; (United Kingdom); Journal Volume: 21:11
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; BIOSYNTHESIS; OPTIMIZATION; CELLULOSE; TRICHODERMA; PHYSIOLOGY; BATCH CULTURE; CARBON; CONTINUOUS CULTURE; ENZYMATIC HYDROLYSIS; GROWTH; NITROGEN; OXYGEN; PH VALUE; PRODUCTIVITY; TEMPERATURE DEPENDENCE; UPTAKE; CARBOHYDRATES; CHEMICAL REACTIONS; CRYOGENIC FLUIDS; DECOMPOSITION; ELEMENTS; FLUIDS; FUNGI; HYDROLYSIS; LYSIS; NONMETALS; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; SYNTHESIS; 550700* - Microbiology; 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
5607507
Research Organizations:
Army Natick Research and Development Command, MA
Country of Origin:
United Kingdom
Language:
English
Contract Number:
E4928-1007
Other Identifying Numbers:
Journal ID: CODEN: BIBIA
Submitting Site:
TIC
Size:
Pages: 1887-1903
Announcement Date:
Feb 01, 1980

Citation Formats

Ryu, D, Andreotti, R, Mandels, M, Gallo, B, and Reese, E T. Studies on quantitative physiology of Trichoderma reesei with two-stage continuous culture for cellulase production. United Kingdom: N. p., 1979. Web. doi:10.1002/bit.260211102.
Ryu, D, Andreotti, R, Mandels, M, Gallo, B, & Reese, E T. Studies on quantitative physiology of Trichoderma reesei with two-stage continuous culture for cellulase production. United Kingdom. doi:10.1002/bit.260211102.
Ryu, D, Andreotti, R, Mandels, M, Gallo, B, and Reese, E T. 1979. "Studies on quantitative physiology of Trichoderma reesei with two-stage continuous culture for cellulase production." United Kingdom. doi:10.1002/bit.260211102. https://www.osti.gov/servlets/purl/10.1002/bit.260211102.
@misc{etde_5607507,
title = {Studies on quantitative physiology of Trichoderma reesei with two-stage continuous culture for cellulase production}
author = {Ryu, D, Andreotti, R, Mandels, M, Gallo, B, and Reese, E T}
abstractNote = {By employing a two-stage continuous-culture system, some of the more important physiological parameters involved in cellulase biosynthesis have been evaluated with an ultimate objective of designing an optimally controlled cellulase process. The two-stage continuous-culture system was run for a period of 1350 hr with Trichoderma reesei strain MCG-77. The temperature and pH were controlled at 32/sup 0/C and pH 4.5 for the first stage (growth) and 28/sup 0/C and pH 3.5 for the second stage (enzyme production). Lactose was the only carbon source for both stages. The ratio of specific uptake rate of carbon to that of nitrogen, Q(C)/Q(N), that supported good cell growth ranged from 11 to 15, and the ratio for maximum specific enzyme productivity ranged from 5 to 13. The maintenance coefficients determined for oxygen, M/sub 0/, and for carbon source, M/sub c/, are 0.85 mmol O/sub 2//g biomass/hr and 0.14 mmol hexose/g biomass/hr, respectively. The yield constants determined are: Y/sub X/O/ = 32.3 g biomass/mol O/sub 2/, Y/sub X/C/ = 1.1 g biomass/g C or Y/sub X/C/ = 0.44 g biomass/g hexose, Y/sub X/N/ = 12.5 g biomass/g nitrogen for the cell growth stage, and Y/sub X/N/ = 16.6 g biomass/g nitrogen for the enzyme production stage. Enzyme was produced only in the second stage. Volumetric and specific enzyme productivities obtained were 90 IU/liter/hrand 8 IU/g biomass/hr, respectively. The maximum specific enzyme productivity observed was 14.8 IU/g biomass/hr. The optimal dilution rate in the second stage that corresponded to the maximum enzyme productivity was 0.026 approx. 0.028 hr/sup -1/, and the specific growth rate in the second stage that supported maximum specific enzyme productivity was equal to or slightly less than zero.}
doi = {10.1002/bit.260211102}
journal = {Biotechnol. Bioeng.; (United Kingdom)}
volume = {21:11}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Nov}
}