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Microbial electrode sensor for alcohols

Abstract

A microbial electrode consisting of immobilized microorganisms, a gas permeable Teflon membrane, and an oxygen electrode was prepared for the continuous determination of methyl and ethyl alcohols. Immobilized Trichosporon brassicae was employed for a microbial electrode sensor for ethyl alcohol. When a sample solution containing ethyl alcohol was injected into a microbial electrode system, the current of the electrode decreased markedly with time until a steady state was reached. The response time was within 10 min by the steady state method and within 6 min by the pulse method. A linear relationship was observed between the current decrease and the concentration of ethyl alcohol below 22.5 mg/liter. The current was reproducible within +- 6% of the relative error when a sample solution containing 16.5 mg/liter ethyl alcohol. The standard deviation was 0.5 mg/liter in 40 experiments. The selectivity of the microbial electrode sensor for ethyl alcohol was satisfactory. The microbial electrode sensor was applied to a fermentation broth of yeasts and satisfactory comparative results were obtained (correlation coefficient 0.98). The current output of the microbial electrode sensor was almost constant for more than three weeks and 2100 assays. A microbial electrode sensor using immobilized bacteria for methyl alcohol was also  More>>
Authors:
Hikuma, M; [1]  Kubo, T; Yasuda, T; Karube, I; Suzuki, S
  1. Ajinomoto Co., Inc., Kawasaki, Japan
Publication Date:
Oct 01, 1979
Product Type:
Journal Article
Reference Number:
EDB-80-018179
Resource Relation:
Journal Name: Biotechnol. Bioeng.; (United Kingdom); Journal Volume: 21:10
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; ALCOHOLS; BIOASSAY; ELECTRODES; FERMENTATION; ON-LINE MEASUREMENT SYSTEMS; BACTERIA; CONTINUOUS CULTURE; ETHANOL; METHANOL; MICROORGANISMS; OXYGEN; TEFLON; YEASTS; BIOCONVERSION; CRYOGENIC FLUIDS; ELEMENTS; FLUIDS; FLUORINATED ALIPHATIC HYDROCARBONS; FUNGI; HALOGENATED ALIPHATIC HYDROCARBONS; HYDROXY COMPOUNDS; NONMETALS; ON-LINE SYSTEMS; ORGANIC COMPOUNDS; ORGANIC FLUORINE COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC POLYMERS; PETROCHEMICALS; PETROLEUM PRODUCTS; PLANTS; PLASTICS; POLYETHYLENES; POLYMERS; POLYOLEFINS; POLYTETRAFLUOROETHYLENE; 550700* - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
OSTI ID:
5607468
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BIBIA
Submitting Site:
TIC
Size:
Pages: 1845-1853
Announcement Date:
Feb 01, 1980

Citation Formats

Hikuma, M, Kubo, T, Yasuda, T, Karube, I, and Suzuki, S. Microbial electrode sensor for alcohols. United Kingdom: N. p., 1979. Web. doi:10.1002/bit.260211011.
Hikuma, M, Kubo, T, Yasuda, T, Karube, I, & Suzuki, S. Microbial electrode sensor for alcohols. United Kingdom. doi:10.1002/bit.260211011.
Hikuma, M, Kubo, T, Yasuda, T, Karube, I, and Suzuki, S. 1979. "Microbial electrode sensor for alcohols." United Kingdom. doi:10.1002/bit.260211011. https://www.osti.gov/servlets/purl/10.1002/bit.260211011.
@misc{etde_5607468,
title = {Microbial electrode sensor for alcohols}
author = {Hikuma, M, Kubo, T, Yasuda, T, Karube, I, and Suzuki, S}
abstractNote = {A microbial electrode consisting of immobilized microorganisms, a gas permeable Teflon membrane, and an oxygen electrode was prepared for the continuous determination of methyl and ethyl alcohols. Immobilized Trichosporon brassicae was employed for a microbial electrode sensor for ethyl alcohol. When a sample solution containing ethyl alcohol was injected into a microbial electrode system, the current of the electrode decreased markedly with time until a steady state was reached. The response time was within 10 min by the steady state method and within 6 min by the pulse method. A linear relationship was observed between the current decrease and the concentration of ethyl alcohol below 22.5 mg/liter. The current was reproducible within +- 6% of the relative error when a sample solution containing 16.5 mg/liter ethyl alcohol. The standard deviation was 0.5 mg/liter in 40 experiments. The selectivity of the microbial electrode sensor for ethyl alcohol was satisfactory. The microbial electrode sensor was applied to a fermentation broth of yeasts and satisfactory comparative results were obtained (correlation coefficient 0.98). The current output of the microbial electrode sensor was almost constant for more than three weeks and 2100 assays. A microbial electrode sensor using immobilized bacteria for methyl alcohol was also described.}
doi = {10.1002/bit.260211011}
journal = {Biotechnol. Bioeng.; (United Kingdom)}
volume = {21:10}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Oct}
}