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Investigation on the pancreatic and stomach secretion in pigs by means of continuous infusion of /sup 14/C-amino acids

Journal Article:

Abstract

2 pigs received a barley-soya bean meal diet and another 2 a casein-wheat starch diet. The specific radioactivity (SR = dpm/..mu..mol) of leucine and phenylalanine in the TCA soluble fraction of plasma and in the TCA soluble and TCA precipitable fractions of pancreatic juice and of digesta leaving the stomach was determined during 6 hours of intravenous infusion of /sup 14/C-leucine and /sup 14/C-phenylalanine. At the end of the infusion the SR of both amino acids in both fractions of several tissues was measured and used for calculations of the rate of tissue protein synthesis. The results are that mainly amino acids derived from the extracellular space were used for synthesis that the process of synthesis, concentration and secretion of secretory proteins requires in pigs 120 to 180 minutes, and that TCA soluble amino acids in pancreatic juice are not free amino acids per se, but originate from processing of presecretory proteins. In the duodenal digesta labelled proteins appeared 3 to 4 hours after the beginning of the infusion. Both, secretion of proteins by the pancreas and by the stomach seemed to be more stimulated after feeding the barley-soya diet than the casein-starch diet. Of all tissues, the SR of  More>>
Authors:
Simon, O; Bergner, H; Muenchmeyer, R; [1]  Zebrowska, T [2] 
  1. Humboldt-Universitaet, Berlin (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin
  2. Polska Akademia Nauk, Warsaw
Publication Date:
Jan 01, 1983
Product Type:
Journal Article
Reference Number:
AIX-14-775788; EDB-83-180009
Resource Relation:
Journal Name: Arch. Tierernaehr.; (German Democratic Republic); Journal Volume: 33:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; PANCREAS; SECRETION; STOMACH; BIOLOGICAL LOCALIZATION; BIOLOGICAL MATERIALS; BIOSYNTHESIS; BLOOD PLASMA; CARBON 14 COMPOUNDS; DIET; DIGESTION; INFUSION; LARGE INTESTINE; LEUCINE; LIVER; MUCOUS MEMBRANES; MUSCLES; PHENYLALANINE; PROTEINS; RADIONUCLIDE KINETICS; SMALL INTESTINE; SWINE; TIME DEPENDENCE; TISSUES; TRACER TECHNIQUES; UPTAKE; AMINO ACIDS; ANIMALS; BLOOD; BODY; BODY FLUIDS; CARBOXYLIC ACIDS; DIGESTIVE SYSTEM; DOMESTIC ANIMALS; ENDOCRINE GLANDS; GASTROINTESTINAL TRACT; GLANDS; INTAKE; INTESTINES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; MEMBRANES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; SYNTHESIS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
5598719
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ARTIA
Submitting Site:
HEDB
Size:
Pages: 9-22
Announcement Date:

Journal Article:

Citation Formats

Simon, O, Bergner, H, Muenchmeyer, R, and Zebrowska, T. Investigation on the pancreatic and stomach secretion in pigs by means of continuous infusion of /sup 14/C-amino acids. Germany: N. p., 1983. Web.
Simon, O, Bergner, H, Muenchmeyer, R, & Zebrowska, T. Investigation on the pancreatic and stomach secretion in pigs by means of continuous infusion of /sup 14/C-amino acids. Germany.
Simon, O, Bergner, H, Muenchmeyer, R, and Zebrowska, T. 1983. "Investigation on the pancreatic and stomach secretion in pigs by means of continuous infusion of /sup 14/C-amino acids." Germany.
@misc{etde_5598719,
title = {Investigation on the pancreatic and stomach secretion in pigs by means of continuous infusion of /sup 14/C-amino acids}
author = {Simon, O, Bergner, H, Muenchmeyer, R, and Zebrowska, T}
abstractNote = {2 pigs received a barley-soya bean meal diet and another 2 a casein-wheat starch diet. The specific radioactivity (SR = dpm/..mu..mol) of leucine and phenylalanine in the TCA soluble fraction of plasma and in the TCA soluble and TCA precipitable fractions of pancreatic juice and of digesta leaving the stomach was determined during 6 hours of intravenous infusion of /sup 14/C-leucine and /sup 14/C-phenylalanine. At the end of the infusion the SR of both amino acids in both fractions of several tissues was measured and used for calculations of the rate of tissue protein synthesis. The results are that mainly amino acids derived from the extracellular space were used for synthesis that the process of synthesis, concentration and secretion of secretory proteins requires in pigs 120 to 180 minutes, and that TCA soluble amino acids in pancreatic juice are not free amino acids per se, but originate from processing of presecretory proteins. In the duodenal digesta labelled proteins appeared 3 to 4 hours after the beginning of the infusion. Both, secretion of proteins by the pancreas and by the stomach seemed to be more stimulated after feeding the barley-soya diet than the casein-starch diet. Of all tissues, the SR of amino acids in proteins was highest in the pancreas. However, proteins secreted by the pancreas were 3 to 4 times higher labelled than those retained in the tissue. The range of the fractional rate of protein synthesis was calculated for the sections of the gastrointestinal tract, the liver and skeletal muscles and discussed with other findings.}
journal = {Arch. Tierernaehr.; (German Democratic Republic)}
volume = {33:1}
journal type = {AC}
place = {Germany}
year = {1983}
month = {Jan}
}