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Monitoring and control of enzymic sucrose hydrolysis using on-line biosensors

Journal Article:

Abstract

Previously reported flow microcalorimeter devices for enzymic reaction heat measurement, enzyme thermistors, have here been extended with systems for on-line sample treatment. Glucose analysis was performed by intermittent flow injection of 50 ..mu..l samples through such an enzyme thermistor device containing immobilized glucose oxidase and catalase. Sucrose analysis was performed by allowing diluted samples to continuously pass through an additional enzyme thermistor containing immobilized invertase. The reaction heats were recorded as temperature changes in the order of 10-50 m degrees C for concentration of 0.05-0.30 M glucose or sucrose present in the original non-diluted samples. The performance of this system was investigated by its ability to follow concentration changes obtained from a gradient mixer. The system was applied to monitoring and controlling the hydrolysis of sucrose to glucose and fructose in a plug-flow reactor with immobilized invertase. The reactor was continuously fed by a flow of sucrose of up to 0.3 M (100 g/l). Glucose and remaining sucrose were monitored in the effluent of the column. By using flow rate controlled feed pumps for sucrose and diluent the influent concentration of sucrose was varied while the overall flow rate remained constant. On-line control of the effluent concentration of glucose and  More>>
Publication Date:
Feb 01, 1985
Product Type:
Journal Article
Reference Number:
ERA-11-040102; EDB-86-126313
Resource Relation:
Journal Name: Appl. Microbiol. Biotechnol.; (Germany, Federal Republic of); Journal Volume: 21:3/4
Subject:
09 BIOMASS FUELS; ETHANOL PLANTS; ON-LINE CONTROL SYSTEMS; ON-LINE MEASUREMENT SYSTEMS; GLUCOSE; MONITORING; SACCHAROSE; ENZYMATIC HYDROLYSIS; CALORIMETRY; IMMOBILIZED ENZYMES; MICROPROCESSORS; PERFORMANCE; PROCESS CONTROL; REACTION HEAT; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; COMPUTERS; CONTROL; CONTROL SYSTEMS; DECOMPOSITION; DISACCHARIDES; ELECTRONIC CIRCUITS; ENTHALPY; HEXOSES; HYDROLYSIS; INDUSTRIAL PLANTS; LYSIS; MICROELECTRONIC CIRCUITS; MONOSACCHARIDES; OLIGOSACCHARIDES; ON-LINE SYSTEMS; ORGANIC COMPOUNDS; PHYSICAL PROPERTIES; SACCHARIDES; SOLVOLYSIS; THERMODYNAMIC PROPERTIES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
5583098
Research Organizations:
Univ. of Lund, Sweden
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: AMBID
Submitting Site:
HEDB
Size:
Pages: 135-142
Announcement Date:

Journal Article:

Citation Formats

Mandenius, C F, Buelow, L, Danielsson, B, and Mosbach, K. Monitoring and control of enzymic sucrose hydrolysis using on-line biosensors. Germany: N. p., 1985. Web.
Mandenius, C F, Buelow, L, Danielsson, B, & Mosbach, K. Monitoring and control of enzymic sucrose hydrolysis using on-line biosensors. Germany.
Mandenius, C F, Buelow, L, Danielsson, B, and Mosbach, K. 1985. "Monitoring and control of enzymic sucrose hydrolysis using on-line biosensors." Germany.
@misc{etde_5583098,
title = {Monitoring and control of enzymic sucrose hydrolysis using on-line biosensors}
author = {Mandenius, C F, Buelow, L, Danielsson, B, and Mosbach, K}
abstractNote = {Previously reported flow microcalorimeter devices for enzymic reaction heat measurement, enzyme thermistors, have here been extended with systems for on-line sample treatment. Glucose analysis was performed by intermittent flow injection of 50 ..mu..l samples through such an enzyme thermistor device containing immobilized glucose oxidase and catalase. Sucrose analysis was performed by allowing diluted samples to continuously pass through an additional enzyme thermistor containing immobilized invertase. The reaction heats were recorded as temperature changes in the order of 10-50 m degrees C for concentration of 0.05-0.30 M glucose or sucrose present in the original non-diluted samples. The performance of this system was investigated by its ability to follow concentration changes obtained from a gradient mixer. The system was applied to monitoring and controlling the hydrolysis of sucrose to glucose and fructose in a plug-flow reactor with immobilized invertase. The reactor was continuously fed by a flow of sucrose of up to 0.3 M (100 g/l). Glucose and remaining sucrose were monitored in the effluent of the column. By using flow rate controlled feed pumps for sucrose and diluent the influent concentration of sucrose was varied while the overall flow rate remained constant. On-line control of the effluent concentration of glucose and sucrose was achieved by a proportional and integral regulator implemented on a microcomputer. Present concentration of glucose in the effluent could be maintained over an extended period of time despite changes in the overall capacity of the invertase reactor. Long delay times in the sensor system and the enzyme column made it necessary to carefully tune the control parameters. Changes of set-point value and temperature disturbances were used to verify accuracy of controlling performance. 32 references.}
journal = {Appl. Microbiol. Biotechnol.; (Germany, Federal Republic of)}
volume = {21:3/4}
journal type = {AC}
place = {Germany}
year = {1985}
month = {Feb}
}