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Adenine-N-oxide produced from adenine with gamma-rays and its binding to SH protein

Journal Article:

Abstract

/sup 14/C-labeled adenine aqueous solution was irradiated with /sup 60/Co gamma-rays. The yield of adenine-7-N-oxide, a radiolytic product, was determined by Sephadex G-10 column chromatography and TLC autoradiography. The apparent productive yield was very low, but the true yield should be much higher because of the reversible reaction to adenine and the easy decomposition of the N-oxide itself. Using synthesized /sup 14/C-adenine-7-N-oxide, noncovalent binding of this N-oxide to urease, an SH protein, was confirmed in comparison between the presence and absence of SDS by Ultrogel AcA 22 column chromatography. The noncovalent binding of the gamma-irradiated /sup 35/S-cysteine was also observed. The yield reached a limit in O/sub 2/ easier than in N/sub 2/ as the atmosphere for DNA irradiation. These results support an interaction structure, chemical bonds N-O---H-S-, for noncovalent binding which may be applied to the biological system as a radiation-induced damage.
Authors:
Yamamoto, O [1] 
  1. Hiroshima Univ. (Japan). Research Inst. for Nuclear Medicine and Biology
Publication Date:
Dec 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-13-668520; EDB-82-090641
Resource Relation:
Journal Name: J. Radiat. Res.; (Japan); Journal Volume: 21:3-4
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ADENINES; RADIOLYSIS; CARBON 14 COMPOUNDS; CHEMICAL REACTION YIELD; COVALENCE; CROSS-LINKING; CYSTEINE; DNA; GAMMA RADIATION; IRRADIATION; RADIOCHROMATOGRAPHY; SULFHYDRYL RADICALS; UREASE; AMIDINASES; AMINES; AMINO ACIDS; ANTIMETABOLITES; CARBOXYLIC ACIDS; CHEMICAL RADIATION EFFECTS; CHEMICAL REACTIONS; CHEMISTRY; CHROMATOGRAPHY; DECOMPOSITION; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC COMPOUNDS; HYDROLASES; IONIZING RADIATIONS; LABELLED COMPOUNDS; NON-PEPTIDE C-N HYDROLASES; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; POLYMERIZATION; PURINES; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RADICALS; SEPARATION PROCESSES; THIOLS; YIELDS; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
5579541
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JRARA
Submitting Site:
HEDB
Size:
Pages: 239-247
Announcement Date:

Journal Article:

Citation Formats

Yamamoto, O. Adenine-N-oxide produced from adenine with gamma-rays and its binding to SH protein. Japan: N. p., 1980. Web.
Yamamoto, O. Adenine-N-oxide produced from adenine with gamma-rays and its binding to SH protein. Japan.
Yamamoto, O. 1980. "Adenine-N-oxide produced from adenine with gamma-rays and its binding to SH protein." Japan.
@misc{etde_5579541,
title = {Adenine-N-oxide produced from adenine with gamma-rays and its binding to SH protein}
author = {Yamamoto, O}
abstractNote = {/sup 14/C-labeled adenine aqueous solution was irradiated with /sup 60/Co gamma-rays. The yield of adenine-7-N-oxide, a radiolytic product, was determined by Sephadex G-10 column chromatography and TLC autoradiography. The apparent productive yield was very low, but the true yield should be much higher because of the reversible reaction to adenine and the easy decomposition of the N-oxide itself. Using synthesized /sup 14/C-adenine-7-N-oxide, noncovalent binding of this N-oxide to urease, an SH protein, was confirmed in comparison between the presence and absence of SDS by Ultrogel AcA 22 column chromatography. The noncovalent binding of the gamma-irradiated /sup 35/S-cysteine was also observed. The yield reached a limit in O/sub 2/ easier than in N/sub 2/ as the atmosphere for DNA irradiation. These results support an interaction structure, chemical bonds N-O---H-S-, for noncovalent binding which may be applied to the biological system as a radiation-induced damage.}
journal = {J. Radiat. Res.; (Japan)}
volume = {21:3-4}
journal type = {AC}
place = {Japan}
year = {1980}
month = {Dec}
}