Abstract
Cells subjected to nucleoside incorporation studies using radiolabelled materials may suffer radiation damage that can alter the results. Scintimetric and cytofluorographic analyses were performed to confirm this and to determine the optimal experimental doses of, and exposure times to, (/sup 3/H)-TdR, in order to prevent or minimize such radiation damage to cells. The results showed that cultures of human mononuclear cells should be pulsed with 0.125 ..mu..Ci for 14 hr when stimulated with phytohaemagglutinin, 0.125 ..mu..Ci for 18 hr when stimulated with pokeweed mitogen, 0.5 ..mu..Ci for 8 hr when stimulated with concanavalin A and 0.5 ..mu..Ci for 8 hr when subjected to allogeneic stimulus, in order to achieve optimal incorporation with minimal disturbances of the cell cycle.
Ruiz-Arguelles, A;
Llorente, L;
Diaz-Jouanen, E;
Alarcon-Segovia, D
[1]
- Instituto Nacional de la Nutricion, Mexico City
Citation Formats
Ruiz-Arguelles, A, Llorente, L, Diaz-Jouanen, E, and Alarcon-Segovia, D.
Optimal conditions in (/sup 3/H)-thymidine uptake studies to prevent radiation damage to cells. A scintimetric and cytofluorographic analysis.
United Kingdom: N. p.,
1981.
Web.
Ruiz-Arguelles, A, Llorente, L, Diaz-Jouanen, E, & Alarcon-Segovia, D.
Optimal conditions in (/sup 3/H)-thymidine uptake studies to prevent radiation damage to cells. A scintimetric and cytofluorographic analysis.
United Kingdom.
Ruiz-Arguelles, A, Llorente, L, Diaz-Jouanen, E, and Alarcon-Segovia, D.
1981.
"Optimal conditions in (/sup 3/H)-thymidine uptake studies to prevent radiation damage to cells. A scintimetric and cytofluorographic analysis."
United Kingdom.
@misc{etde_5579441,
title = {Optimal conditions in (/sup 3/H)-thymidine uptake studies to prevent radiation damage to cells. A scintimetric and cytofluorographic analysis}
author = {Ruiz-Arguelles, A, Llorente, L, Diaz-Jouanen, E, and Alarcon-Segovia, D}
abstractNote = {Cells subjected to nucleoside incorporation studies using radiolabelled materials may suffer radiation damage that can alter the results. Scintimetric and cytofluorographic analyses were performed to confirm this and to determine the optimal experimental doses of, and exposure times to, (/sup 3/H)-TdR, in order to prevent or minimize such radiation damage to cells. The results showed that cultures of human mononuclear cells should be pulsed with 0.125 ..mu..Ci for 14 hr when stimulated with phytohaemagglutinin, 0.125 ..mu..Ci for 18 hr when stimulated with pokeweed mitogen, 0.5 ..mu..Ci for 8 hr when stimulated with concanavalin A and 0.5 ..mu..Ci for 8 hr when subjected to allogeneic stimulus, in order to achieve optimal incorporation with minimal disturbances of the cell cycle.}
journal = []
volume = {44:4}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {Dec}
}
title = {Optimal conditions in (/sup 3/H)-thymidine uptake studies to prevent radiation damage to cells. A scintimetric and cytofluorographic analysis}
author = {Ruiz-Arguelles, A, Llorente, L, Diaz-Jouanen, E, and Alarcon-Segovia, D}
abstractNote = {Cells subjected to nucleoside incorporation studies using radiolabelled materials may suffer radiation damage that can alter the results. Scintimetric and cytofluorographic analyses were performed to confirm this and to determine the optimal experimental doses of, and exposure times to, (/sup 3/H)-TdR, in order to prevent or minimize such radiation damage to cells. The results showed that cultures of human mononuclear cells should be pulsed with 0.125 ..mu..Ci for 14 hr when stimulated with phytohaemagglutinin, 0.125 ..mu..Ci for 18 hr when stimulated with pokeweed mitogen, 0.5 ..mu..Ci for 8 hr when stimulated with concanavalin A and 0.5 ..mu..Ci for 8 hr when subjected to allogeneic stimulus, in order to achieve optimal incorporation with minimal disturbances of the cell cycle.}
journal = []
volume = {44:4}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {Dec}
}