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Investigation of benzo(a)pyrene-globin adducts

Abstract

The nature of the adducts formed between benzo(a)pyrene (BP) and globin were investigated in animals treated with (/sup 3/H)BP. Modification levels on globin were determined by radioactivity measurements. Since BP tetraols can be released from benzo(a)pyrene diol epoxide modified protein and DNA by acid treatment, globin samples were treated with acid, released tetraols separated by HPLC and quantitated by scintillation counting. In addition, acid released material was measured in competitive enzyme linked immunosorbent assay (ELISA) using antibodies which recognize BP tetraols. Both measurements indicate that only 2% of bound radioactivity could be released as free BP tetraols. These studies indicate that benzo(a)pyrene diol epoxide may not be the major metabolite of BP involved in globin binding. (author). 14 refs.
Publication Date:
May 01, 1987
Product Type:
Journal Article
Reference Number:
AIX-19-022021; EDB-88-062051
Resource Relation:
Journal Name: Cancer Lett. (Shannon); (Ireland); Journal Volume: 35:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ADDUCTS; BENZOPYRENE; ANIMALS; BIOLOGICAL LOCALIZATION; CARCINOGENS; CHROMATOGRAPHY; GLOBIN; LABELLING; SCINTILLATION COUNTING; AROMATICS; CONDENSED AROMATICS; COUNTING TECHNIQUES; HYDROCARBONS; ORGANIC COMPOUNDS; PROTEINS; SEPARATION PROCESSES; 560162* - Radionuclide Effects, Kinetics, & Toxicology- Animals, Plants, Microorganisms, & Cells
OSTI ID:
5551828
Research Organizations:
Columbia Univ., New York, USA. Cancer Centre/Institute of Cancer Research
Country of Origin:
Ireland
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CALED
Submitting Site:
INIS
Size:
Pages: 139-146
Announcement Date:

Citation Formats

Wallin, H, Jeffre, A M, and Santella, R M. Investigation of benzo(a)pyrene-globin adducts. Ireland: N. p., 1987. Web. doi:10.1016/0304-3835(87)90037-1.
Wallin, H, Jeffre, A M, & Santella, R M. Investigation of benzo(a)pyrene-globin adducts. Ireland. doi:10.1016/0304-3835(87)90037-1.
Wallin, H, Jeffre, A M, and Santella, R M. 1987. "Investigation of benzo(a)pyrene-globin adducts." Ireland. doi:10.1016/0304-3835(87)90037-1. https://www.osti.gov/servlets/purl/10.1016/0304-3835(87)90037-1.
@misc{etde_5551828,
title = {Investigation of benzo(a)pyrene-globin adducts}
author = {Wallin, H, Jeffre, A M, and Santella, R M}
abstractNote = {The nature of the adducts formed between benzo(a)pyrene (BP) and globin were investigated in animals treated with (/sup 3/H)BP. Modification levels on globin were determined by radioactivity measurements. Since BP tetraols can be released from benzo(a)pyrene diol epoxide modified protein and DNA by acid treatment, globin samples were treated with acid, released tetraols separated by HPLC and quantitated by scintillation counting. In addition, acid released material was measured in competitive enzyme linked immunosorbent assay (ELISA) using antibodies which recognize BP tetraols. Both measurements indicate that only 2% of bound radioactivity could be released as free BP tetraols. These studies indicate that benzo(a)pyrene diol epoxide may not be the major metabolite of BP involved in globin binding. (author). 14 refs.}
doi = {10.1016/0304-3835(87)90037-1}
journal = {Cancer Lett. (Shannon); (Ireland)}
volume = {35:2}
journal type = {AC}
place = {Ireland}
year = {1987}
month = {May}
}