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Citric acid by fermentation

Patent:

Abstract

Citric acid is produced in 2 stages, in the first of which Escherichia coli KG93 F-is cultured on a substrate consisting of whey permeate to which phosphate and nitrate or urea is added. In the second stage, Hansenula wickerhamii CBS 4308 is cultured on the culture solution from the first stage and the citric acid isolated in the usual manner. Thus, a whey permeate comprising fat 0.20, protein 0.05, lactose 5.0, lactic acid 0.1, and salts 0.6% (Ca 0.06, P 0.06, K 0.16, N 0.03, and NaCl 0.3%) was amended with 1.2 g phosphate/L and 1.0 g NO/sub 3/sup -///L. After introducing the whey permeate into a continuous fermentor, it was inoculated with E. coli K693 F- and allowed to incubate at 37/sup 0/ and pH 7 (Maintained with NH/sub 3/ gas) for 12 h with aeration, thereby producing concentration pyruvic acid. After an additional 6 h cultivation without aeration, the broth was transferred to another fermentor and there inoculated with H. wickerhamii CBS 4308. The second cultivation step was carried out at 30/sup 0/ and a pH of 5 for 24 h with the pH stabilized by NH/sub 3/ gas. To the second stage culture, 0.3 g Fe(CN)/sup 4  More>>
Authors:
Publication Date:
Nov 30, 1978
Product Type:
Patent
Report Number:
DE 2821762
Reference Number:
EDB-80-048594
Resource Relation:
Patent Priority Date: Priority date 18 May 1977, Sweden
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CITRIC ACID; BIOSYNTHESIS; WHEY; FERMENTATION; AMMONIA; BACTERIA; CHEMICAL REACTION YIELD; ESCHERICHIA COLI; NITRATES; PHOSPHATES; PYRUVIC ACID; BIOCONVERSION; CARBOXYLIC ACIDS; HYDRIDES; HYDROGEN COMPOUNDS; HYDROXY ACIDS; INDUSTRIAL WASTES; KETO ACIDS; MICROORGANISMS; NITROGEN COMPOUNDS; NITROGEN HYDRIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; SYNTHESIS; WASTES; YIELDS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550700 - Microbiology
OSTI ID:
5547284
Country of Origin:
Germany
Language:
English
Submitting Site:
IEA
Size:
Pages: 13
Announcement Date:

Patent:

Citation Formats

El-Sayed, R M. Citric acid by fermentation. Germany: N. p., 1978. Web.
El-Sayed, R M. Citric acid by fermentation. Germany.
El-Sayed, R M. 1978. "Citric acid by fermentation." Germany.
@misc{etde_5547284,
title = {Citric acid by fermentation}
author = {El-Sayed, R M}
abstractNote = {Citric acid is produced in 2 stages, in the first of which Escherichia coli KG93 F-is cultured on a substrate consisting of whey permeate to which phosphate and nitrate or urea is added. In the second stage, Hansenula wickerhamii CBS 4308 is cultured on the culture solution from the first stage and the citric acid isolated in the usual manner. Thus, a whey permeate comprising fat 0.20, protein 0.05, lactose 5.0, lactic acid 0.1, and salts 0.6% (Ca 0.06, P 0.06, K 0.16, N 0.03, and NaCl 0.3%) was amended with 1.2 g phosphate/L and 1.0 g NO/sub 3/sup -///L. After introducing the whey permeate into a continuous fermentor, it was inoculated with E. coli K693 F- and allowed to incubate at 37/sup 0/ and pH 7 (Maintained with NH/sub 3/ gas) for 12 h with aeration, thereby producing concentration pyruvic acid. After an additional 6 h cultivation without aeration, the broth was transferred to another fermentor and there inoculated with H. wickerhamii CBS 4308. The second cultivation step was carried out at 30/sup 0/ and a pH of 5 for 24 h with the pH stabilized by NH/sub 3/ gas. To the second stage culture, 0.3 g Fe(CN)/sup 4 -//L was added. The yield of pyruvic acid in the first stage was 32 g/L and the final yield of citric acid was 43 g/L.}
place = {Germany}
year = {1978}
month = {Nov}
}