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Pilot plant production of glucose from starch with soluble. cap alpha. -amylase and immobilized glucoamylase

Journal Article:

Abstract

Pilot plant studies were conducted on cooking and thinning of corn starch with free ..cap alpha..-amylase and the conversion of the resulting dextrin with immobilized glucoamylase adsorbed on porous SiO/sub 2/. Feeds of intermediate DE values gave maximum yields unless the flow rate of low DE feeds was decreased. Final DE values and glucose concentrations after dextrin treated with Thermamyl 60 ..cap alpha..-amylase had been further hydrolyzed in an immobilized glucoamylase column, were slightly lower than they were when free glucoamylase was used. Similar results were obtained when dextrin, thinned with HT-1000 ..cap alpha..-amylase, was hydrolyzed at 38/sup 0/ and pH 4.4 in the immobilized glucoamylase column. Free glucoamylase yielded values of DE and glucose almost identical with dextrin thinned with Thermamyl 60 ..cap alpha..-amylase. Yields with the free glucoamylase were also slightly higher than they were with SiO/sub 2/-bound enzyme.
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
EDB-80-048599
Resource Relation:
Journal Name: Enzyme Eng.; (United States); Journal Volume: 3
Subject:
09 BIOMASS FUELS; GLUCOSE; BIOSYNTHESIS; STARCH; ENZYMATIC HYDROLYSIS; AMYLASE; CHEMICAL REACTION YIELD; DEXTRIN; IMMOBILIZED ENZYMES; PILOT PLANTS; SILICON OXIDES; ALDEHYDES; CARBOHYDRATES; CHALCOGENIDES; CHEMICAL REACTIONS; DECOMPOSITION; ENZYMES; FUNCTIONAL MODELS; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MONOSACCHARIDES; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; POLYSACCHARIDES; REAGENTS; SACCHARIDES; SILICON COMPOUNDS; SOLVOLYSIS; SYNTHESIS; YIELDS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
5547273
Research Organizations:
Iowa State Univ., Ames
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ENEND
Submitting Site:
IEA
Size:
Pages: 525-530
Announcement Date:

Journal Article:

Citation Formats

Lee, D D, Reilly, P J, and Collins, Jr, E V. Pilot plant production of glucose from starch with soluble. cap alpha. -amylase and immobilized glucoamylase. United States: N. p., 1975. Web.
Lee, D D, Reilly, P J, & Collins, Jr, E V. Pilot plant production of glucose from starch with soluble. cap alpha. -amylase and immobilized glucoamylase. United States.
Lee, D D, Reilly, P J, and Collins, Jr, E V. 1975. "Pilot plant production of glucose from starch with soluble. cap alpha. -amylase and immobilized glucoamylase." United States.
@misc{etde_5547273,
title = {Pilot plant production of glucose from starch with soluble. cap alpha. -amylase and immobilized glucoamylase}
author = {Lee, D D, Reilly, P J, and Collins, Jr, E V}
abstractNote = {Pilot plant studies were conducted on cooking and thinning of corn starch with free ..cap alpha..-amylase and the conversion of the resulting dextrin with immobilized glucoamylase adsorbed on porous SiO/sub 2/. Feeds of intermediate DE values gave maximum yields unless the flow rate of low DE feeds was decreased. Final DE values and glucose concentrations after dextrin treated with Thermamyl 60 ..cap alpha..-amylase had been further hydrolyzed in an immobilized glucoamylase column, were slightly lower than they were when free glucoamylase was used. Similar results were obtained when dextrin, thinned with HT-1000 ..cap alpha..-amylase, was hydrolyzed at 38/sup 0/ and pH 4.4 in the immobilized glucoamylase column. Free glucoamylase yielded values of DE and glucose almost identical with dextrin thinned with Thermamyl 60 ..cap alpha..-amylase. Yields with the free glucoamylase were also slightly higher than they were with SiO/sub 2/-bound enzyme.}
journal = {Enzyme Eng.; (United States)}
volume = {3}
journal type = {AC}
place = {United States}
year = {1975}
month = {Jan}
}