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Measurement of plasma canine C peptide by radioimmunoassay

Journal Article:

Abstract

A sensitive radioimmunoassay for canine C peptide (CCP) was established using synthetic CCP, a specific antiserum, and rabbit anti-guinea pig serum. Radioiodination was performed according to a modified chloramine T method. Tracer preparations have been used for 6 weeks after iodination. The standard curve ranges from 0.028 to 3.0 nmol/l. The intra-assay coefficient of variation (CV) was 3-5% and the inter-assay CV was 6-9% in the optimal range between 0.3 and 0.8 nmol/l. The average recovery of CCP added to plasma samples was 100.6% (n = 9). Canine insulin, porcine proinsulin, bovine proinsulin, and human C peptide exhibited no cross-reactivity. The mean fasting plasma CCP concentration was 0.089 +- 0.021 nmol/l in normal dogs and -0.005 +- 0.007 nmol/l (mean +- SEM) in diabetic dogs, respectively.
Publication Date:
Dec 01, 1985
Product Type:
Journal Article
Reference Number:
AIX-17-047111; EDB-86-121889
Resource Relation:
Journal Name: Exp. Clin. Endocrinol.; (German Democratic Republic); Journal Volume: 86:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; DIABETES MELLITUS; DIAGNOSTIC TECHNIQUES; KININS; RADIOIMMUNOASSAY; PEPTIDES; ACCURACY; BLOOD CHEMISTRY; DOGS; INSULIN; IODINE 125; SENSITIVITY; SPECIFICITY; ANIMALS; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE DISEASES; HORMONES; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; METABOLIC DISEASES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; POLYPEPTIDES; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5530154
Research Organizations:
Zentralinstitut fuer Diabetes, Karlsburg, German Democratic Republic
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EXCED
Submitting Site:
INIS
Size:
Pages: 146-150
Announcement Date:
Jul 01, 1986

Journal Article:

Citation Formats

Besch, W, Woltanski, K P, Fischer, U, Kohnert, K D, and Ziegler, M. Measurement of plasma canine C peptide by radioimmunoassay. Germany: N. p., 1985. Web.
Besch, W, Woltanski, K P, Fischer, U, Kohnert, K D, & Ziegler, M. Measurement of plasma canine C peptide by radioimmunoassay. Germany.
Besch, W, Woltanski, K P, Fischer, U, Kohnert, K D, and Ziegler, M. 1985. "Measurement of plasma canine C peptide by radioimmunoassay." Germany.
@misc{etde_5530154,
title = {Measurement of plasma canine C peptide by radioimmunoassay}
author = {Besch, W, Woltanski, K P, Fischer, U, Kohnert, K D, and Ziegler, M}
abstractNote = {A sensitive radioimmunoassay for canine C peptide (CCP) was established using synthetic CCP, a specific antiserum, and rabbit anti-guinea pig serum. Radioiodination was performed according to a modified chloramine T method. Tracer preparations have been used for 6 weeks after iodination. The standard curve ranges from 0.028 to 3.0 nmol/l. The intra-assay coefficient of variation (CV) was 3-5% and the inter-assay CV was 6-9% in the optimal range between 0.3 and 0.8 nmol/l. The average recovery of CCP added to plasma samples was 100.6% (n = 9). Canine insulin, porcine proinsulin, bovine proinsulin, and human C peptide exhibited no cross-reactivity. The mean fasting plasma CCP concentration was 0.089 +- 0.021 nmol/l in normal dogs and -0.005 +- 0.007 nmol/l (mean +- SEM) in diabetic dogs, respectively.}
journal = {Exp. Clin. Endocrinol.; (German Democratic Republic)}
volume = {86:2}
journal type = {AC}
place = {Germany}
year = {1985}
month = {Dec}
}