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Expression of maize prolamins in Escherichia Coli. [Zea mays L]

Journal Article:

Abstract

A cDNA expression library of developing corn (Zea mays L.) endosperm has been constructed using plasmid pUC8 as vector and Escherichia coli strain DH1 as host. The expression library was screened with non-radioactive immunological probes to detect the expression of gamma-zein and alpha-zein. When anti-gamma-zein antibody was used as the probe, 23 colonies gave positive reactions. The lengths of cDNA inserts of the 23 colonies were found to be 250-900 base pairs. When anti-alpha zein antibody was used, however, fewer colonies gave positive reactions. The library was also screened by colony-hybridization with /sup 32/P-labeled DNA probes. Based on immunological and hybridization screening of the library and other evidence, it was conclude that alpha-zein was either toxic to E. coli cells or rapidly degraded whereas gamma-zein and its fragments were readily expressed. 21 references.
Publication Date:
Dec 02, 1985
Product Type:
Journal Article
Reference Number:
EDB-86-138346
Resource Relation:
Journal Name: Plant Sci. Lett.; (Netherlands); Journal Volume: 42:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; ESCHERICHIA COLI; HYBRIDIZATION; ANTIBODIES; CELL CULTURES; CLONING; DNA; ENDOSPERM; GENETICS; IMMUNOLOGY; LABELLED COMPOUNDS; MAIZE; PHOSPHORUS 32; PLANT CELLS; PLASMIDS; PROTEINS; TOXICITY; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGY; BODY; CELL CONSTITUENTS; CEREALS; DAYS LIVING RADIOISOTOPES; GRASS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PLANT TISSUES; PLANTS; RADIOISOTOPES; TISSUES; 550401* - Genetics- Tracer Techniques
OSTI ID:
5528357
Research Organizations:
Virginia Polytechnic Institute and State Univ., Blacksburg
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PTSLA
Submitting Site:
INIS
Size:
Pages: 49-54
Announcement Date:

Journal Article:

Citation Formats

Wang, Szu-zhen, and Esen, Asim. Expression of maize prolamins in Escherichia Coli. [Zea mays L]. Netherlands: N. p., 1985. Web.
Wang, Szu-zhen, & Esen, Asim. Expression of maize prolamins in Escherichia Coli. [Zea mays L]. Netherlands.
Wang, Szu-zhen, and Esen, Asim. 1985. "Expression of maize prolamins in Escherichia Coli. [Zea mays L]." Netherlands.
@misc{etde_5528357,
title = {Expression of maize prolamins in Escherichia Coli. [Zea mays L]}
author = {Wang, Szu-zhen, and Esen, Asim}
abstractNote = {A cDNA expression library of developing corn (Zea mays L.) endosperm has been constructed using plasmid pUC8 as vector and Escherichia coli strain DH1 as host. The expression library was screened with non-radioactive immunological probes to detect the expression of gamma-zein and alpha-zein. When anti-gamma-zein antibody was used as the probe, 23 colonies gave positive reactions. The lengths of cDNA inserts of the 23 colonies were found to be 250-900 base pairs. When anti-alpha zein antibody was used, however, fewer colonies gave positive reactions. The library was also screened by colony-hybridization with /sup 32/P-labeled DNA probes. Based on immunological and hybridization screening of the library and other evidence, it was conclude that alpha-zein was either toxic to E. coli cells or rapidly degraded whereas gamma-zein and its fragments were readily expressed. 21 references.}
journal = {Plant Sci. Lett.; (Netherlands)}
volume = {42:1}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Dec}
}