Abstract
In determining the effects of heavy metals in microbial activity and litter degradation in river sediments, one approach is to determine the effects of these pollutants on sediment enzyme activity and synthesis. Methods to assay amylase, cellulase and urease activity in diverse river sediments are reported. Enzyme activity was low in non-amended sediments, but increased markedly when the appropriate substrate was added, paralleling both athropogenic and natural amendment. Linear relationships between enzyme activity, length of incubation, sample size and substrate concentration were established. Sediment enzyme activity generally obeyed Michaelis-Menton kinetics, but of the three enzymes, urease gave least significant correlation coefficients when the data for substrate concentration versus activity was applied to the Eadie-Hofstee transformation of the Michaelis-Menten equation. K/sub m/ and V/sub max/ for amylase, cellulase and urease in sediments are reported. (JMT)
Citation Formats
Duddridge, J E, and Wainwright, M.
Enzyme activity and kinetics in substrate-amended river sediments.
United Kingdom: N. p.,
1982.
Web.
Duddridge, J E, & Wainwright, M.
Enzyme activity and kinetics in substrate-amended river sediments.
United Kingdom.
Duddridge, J E, and Wainwright, M.
1982.
"Enzyme activity and kinetics in substrate-amended river sediments."
United Kingdom.
@misc{etde_5528267,
title = {Enzyme activity and kinetics in substrate-amended river sediments}
author = {Duddridge, J E, and Wainwright, M}
abstractNote = {In determining the effects of heavy metals in microbial activity and litter degradation in river sediments, one approach is to determine the effects of these pollutants on sediment enzyme activity and synthesis. Methods to assay amylase, cellulase and urease activity in diverse river sediments are reported. Enzyme activity was low in non-amended sediments, but increased markedly when the appropriate substrate was added, paralleling both athropogenic and natural amendment. Linear relationships between enzyme activity, length of incubation, sample size and substrate concentration were established. Sediment enzyme activity generally obeyed Michaelis-Menton kinetics, but of the three enzymes, urease gave least significant correlation coefficients when the data for substrate concentration versus activity was applied to the Eadie-Hofstee transformation of the Michaelis-Menten equation. K/sub m/ and V/sub max/ for amylase, cellulase and urease in sediments are reported. (JMT)}
journal = []
volume = {16:3}
journal type = {AC}
place = {United Kingdom}
year = {1982}
month = {Jan}
}
title = {Enzyme activity and kinetics in substrate-amended river sediments}
author = {Duddridge, J E, and Wainwright, M}
abstractNote = {In determining the effects of heavy metals in microbial activity and litter degradation in river sediments, one approach is to determine the effects of these pollutants on sediment enzyme activity and synthesis. Methods to assay amylase, cellulase and urease activity in diverse river sediments are reported. Enzyme activity was low in non-amended sediments, but increased markedly when the appropriate substrate was added, paralleling both athropogenic and natural amendment. Linear relationships between enzyme activity, length of incubation, sample size and substrate concentration were established. Sediment enzyme activity generally obeyed Michaelis-Menton kinetics, but of the three enzymes, urease gave least significant correlation coefficients when the data for substrate concentration versus activity was applied to the Eadie-Hofstee transformation of the Michaelis-Menten equation. K/sub m/ and V/sub max/ for amylase, cellulase and urease in sediments are reported. (JMT)}
journal = []
volume = {16:3}
journal type = {AC}
place = {United Kingdom}
year = {1982}
month = {Jan}
}