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Iodination of monoclonal antibodies, proteins and peptide using iodogen

Journal Article:

Abstract

The use of the iodinating reagent 1,3,4,6-tetrachloro-3{alpha}, 6{alpha}-diphenylglycholuril (Iodogen) to label monoclonal antibodies (McAbs). Proteins and peptides was invesrigated with McAbs identified as mouse IgG and IgM, arginine-vasopressin (AVP), glucagon (Glu), human insulin(hI) and albumin(Alb). The labeled products were purified by gel chromatography and their immunoreactivity were detected by RIA or IRMA> Comparison of the Iodogen method with the lactoperoxides and chloramine-T methods showed that the Iodogen method had a number of advantages: (1) technically simpler ; (2) a high labeling efficiency could be obtained; (3) the immunoreactivity of the products was minimally affected; (4) the products were stable for up to 4 months.
Authors:
Zhanpo, Niu [1] 
  1. Chinese Academy of Medical Sciences, Beijing, BJ (China). PUMC Hospital; and others
Publication Date:
May 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-20-074999; EDB-89-145861
Resource Relation:
Journal Name: Zhonghua Heyixue Zazhi; (China); Journal Volume: 8:2
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; MONOCLONAL ANTIBODIES; LABELLING; POLYPEPTIDES; PROTEINS; INSULIN; IODINE 125; PERFORMANCE TESTING; STABILITY; ANTIBODIES; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; RADIOISOTOPES; TESTING; 400703* - Radiochemistry & Nuclear Chemistry- Radioisotope Production
OSTI ID:
5521919
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 0253-9780; CODEN: CITCD
Submitting Site:
INIS
Size:
Pages: 98-100
Announcement Date:
Sep 01, 1988

Journal Article:

Citation Formats

Zhanpo, Niu. Iodination of monoclonal antibodies, proteins and peptide using iodogen. China: N. p., 1988. Web.
Zhanpo, Niu. Iodination of monoclonal antibodies, proteins and peptide using iodogen. China.
Zhanpo, Niu. 1988. "Iodination of monoclonal antibodies, proteins and peptide using iodogen." China.
@misc{etde_5521919,
title = {Iodination of monoclonal antibodies, proteins and peptide using iodogen}
author = {Zhanpo, Niu}
abstractNote = {The use of the iodinating reagent 1,3,4,6-tetrachloro-3{alpha}, 6{alpha}-diphenylglycholuril (Iodogen) to label monoclonal antibodies (McAbs). Proteins and peptides was invesrigated with McAbs identified as mouse IgG and IgM, arginine-vasopressin (AVP), glucagon (Glu), human insulin(hI) and albumin(Alb). The labeled products were purified by gel chromatography and their immunoreactivity were detected by RIA or IRMA> Comparison of the Iodogen method with the lactoperoxides and chloramine-T methods showed that the Iodogen method had a number of advantages: (1) technically simpler ; (2) a high labeling efficiency could be obtained; (3) the immunoreactivity of the products was minimally affected; (4) the products were stable for up to 4 months.}
journal = {Zhonghua Heyixue Zazhi; (China)}
volume = {8:2}
journal type = {AC}
place = {China}
year = {1988}
month = {May}
}