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In vitro proliferation of normal and leukemic human leukocytes controlled by an inhibitory endopeptide. [/sup 3/H-TdR incorporation]

Journal Article:

Abstract

GI-3, an endogenous inhibitory fraction isolated from leukocytes, selectively inhibits the proliferation of granuloid precursor cells in a non-toxic manner. Its active principle is an acidic chlor-tolidine positive decapeptide. The in vitro effect on normal and acute leukemic human bone marrow and blood cells was examined. A dose dependent inhibition by GI-3 of /sup 3/H-TdR incorporation into myeloid cells of normal bone marrow was found, the sensitivity of human cells being higher than that of rat cells. The proliferation of the target leukemic bone marrow and blood cells was also decreased by the endogenous inhibitor in a dose-dependent manner in untreated subjects as well as in patients in remission or relapse. The rate of inhibition of leukemic cell proliferation in the short-term suspension system examined almost coincided with the action of well-known cytostatics applied for comparison. Beyond its direct cytostatic effect, GI-3 could be used in the differential diagnosis of blastic leukemias, complementing the routine cytochemical methods.
Publication Date:
Jan 01, 1983
Product Type:
Journal Article
Reference Number:
AIX-14-795451; EDB-83-197722
Resource Relation:
Journal Name: Folia Haematol. (Leipzig); (German Democratic Republic); Journal Volume: 110:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIMITOTIC DRUGS; BIOLOGICAL EFFECTS; LEUKEMIA; CELL PROLIFERATION; LEUKOCYTES; BONE MARROW CELLS; CELL CULTURES; COMPARATIVE EVALUATIONS; DNA; INHIBITION; MAN; PATIENTS; PEPTIDES; RADIONUCLIDE KINETICS; SENSITIVITY; THYMIDINE; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; DRUGS; HEMIC DISEASES; HETEROCYCLIC COMPOUNDS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; NEOPLASMS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PRIMATES; PROTEINS; PYRIMIDINES; RIBOSIDES; SOMATIC CELLS; VERTEBRATES; 550600* - Medicine
OSTI ID:
5519723
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: FOLHA
Submitting Site:
HEDB
Size:
Pages: 24-31
Announcement Date:

Journal Article:

Citation Formats

Balazs, A, Mann, J, Takacsi-Nagy, L, Zimonyi, I, Molnar, A, Klupp, T, Istvan Municipal Hospital, Budapest (Hungary), and Heim Pal Children's Hospital Budapest, (Hungary)). In vitro proliferation of normal and leukemic human leukocytes controlled by an inhibitory endopeptide. [/sup 3/H-TdR incorporation]. Germany: N. p., 1983. Web.
Balazs, A, Mann, J, Takacsi-Nagy, L, Zimonyi, I, Molnar, A, Klupp, T, Istvan Municipal Hospital, Budapest (Hungary), & Heim Pal Children's Hospital Budapest, (Hungary)). In vitro proliferation of normal and leukemic human leukocytes controlled by an inhibitory endopeptide. [/sup 3/H-TdR incorporation]. Germany.
Balazs, A, Mann, J, Takacsi-Nagy, L, Zimonyi, I, Molnar, A, Klupp, T, Istvan Municipal Hospital, Budapest (Hungary), and Heim Pal Children's Hospital Budapest, (Hungary)). 1983. "In vitro proliferation of normal and leukemic human leukocytes controlled by an inhibitory endopeptide. [/sup 3/H-TdR incorporation]." Germany.
@misc{etde_5519723,
title = {In vitro proliferation of normal and leukemic human leukocytes controlled by an inhibitory endopeptide. [/sup 3/H-TdR incorporation]}
author = {Balazs, A, Mann, J, Takacsi-Nagy, L, Zimonyi, I, Molnar, A, Klupp, T, Istvan Municipal Hospital, Budapest (Hungary), and Heim Pal Children's Hospital Budapest, (Hungary))}
abstractNote = {GI-3, an endogenous inhibitory fraction isolated from leukocytes, selectively inhibits the proliferation of granuloid precursor cells in a non-toxic manner. Its active principle is an acidic chlor-tolidine positive decapeptide. The in vitro effect on normal and acute leukemic human bone marrow and blood cells was examined. A dose dependent inhibition by GI-3 of /sup 3/H-TdR incorporation into myeloid cells of normal bone marrow was found, the sensitivity of human cells being higher than that of rat cells. The proliferation of the target leukemic bone marrow and blood cells was also decreased by the endogenous inhibitor in a dose-dependent manner in untreated subjects as well as in patients in remission or relapse. The rate of inhibition of leukemic cell proliferation in the short-term suspension system examined almost coincided with the action of well-known cytostatics applied for comparison. Beyond its direct cytostatic effect, GI-3 could be used in the differential diagnosis of blastic leukemias, complementing the routine cytochemical methods.}
journal = {Folia Haematol. (Leipzig); (German Democratic Republic)}
volume = {110:1}
journal type = {AC}
place = {Germany}
year = {1983}
month = {Jan}
}