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Preparation of radiolabeled bioactive asbestos fibers

Abstract

We have developed an efficient procedure to radiolabel asbestos fibers while retaining the bioactivity of the fibers. The fibers are labeled with {sup 68}Ge. The {sup 68}Ge decays into {sup 68}Ga, which then can be detected by its characteristic positron emission. Both chrysotile and crocidolite asbestos, a serpentine and an amphibole, respectively, were radiolabeled successfully. Mild reaction conditions and short reaction times were found under which {similar to}90% of the added {sup 68}Ge and {sup 68}Ga bound to the fibers. The radiolabel was retained even after washing the fibers extensively with physiologic buffers. The effects of the labeling on the bioactivity of the fibers were evaluated in an in vitro assay using guinea pig alveolar macrophages as a target cell. Labeled chrysotile fibers were found to retain >95% of their ability to stimulate these cells. The labeling procedure described in this study should be useful in preparing labeled fibers to investigate both in vitro and in vivo phenomena. (author).
Authors:
Tewson, T J; Francsechini, M P; Scheule, R K; Holian, A [1] 
  1. Texas Univ., Houston, TX (USA). Health Science Center
Publication Date:
Jan 01, 1991
Product Type:
Journal Article
Reference Number:
AIX-22-068434; EDB-91-118044
Resource Relation:
Journal Name: Applied Radiation and Isotopes (International Journal of Radiation Applications and Instrumentation, Part A); (United Kingdom); Journal Volume: 42:6
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; ASBESTOS; GERMANIUM 68; LABELLING; BIOASSAY; FIBERS; GALLIUM 68; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-EVEN NUCLEI; GALLIUM ISOTOPES; GERMANIUM ISOTOPES; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOTOPES; NUCLEI; ODD-ODD NUCLEI; RADIOISOTOPES; 400703* - Radiochemistry & Nuclear Chemistry- Radioisotope Production
OSTI ID:
5516729
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0883-2889; CODEN: ARISE; Other: CNN: BC-614; ES-04804
Submitting Site:
GBN
Size:
Pages: 499-502
Announcement Date:

Citation Formats

Tewson, T J, Francsechini, M P, Scheule, R K, and Holian, A. Preparation of radiolabeled bioactive asbestos fibers. United Kingdom: N. p., 1991. Web. doi:10.1016/0883-2889(91)90151-P.
Tewson, T J, Francsechini, M P, Scheule, R K, & Holian, A. Preparation of radiolabeled bioactive asbestos fibers. United Kingdom. doi:10.1016/0883-2889(91)90151-P.
Tewson, T J, Francsechini, M P, Scheule, R K, and Holian, A. 1991. "Preparation of radiolabeled bioactive asbestos fibers." United Kingdom. doi:10.1016/0883-2889(91)90151-P. https://www.osti.gov/servlets/purl/10.1016/0883-2889(91)90151-P.
@misc{etde_5516729,
title = {Preparation of radiolabeled bioactive asbestos fibers}
author = {Tewson, T J, Francsechini, M P, Scheule, R K, and Holian, A}
abstractNote = {We have developed an efficient procedure to radiolabel asbestos fibers while retaining the bioactivity of the fibers. The fibers are labeled with {sup 68}Ge. The {sup 68}Ge decays into {sup 68}Ga, which then can be detected by its characteristic positron emission. Both chrysotile and crocidolite asbestos, a serpentine and an amphibole, respectively, were radiolabeled successfully. Mild reaction conditions and short reaction times were found under which {similar to}90% of the added {sup 68}Ge and {sup 68}Ga bound to the fibers. The radiolabel was retained even after washing the fibers extensively with physiologic buffers. The effects of the labeling on the bioactivity of the fibers were evaluated in an in vitro assay using guinea pig alveolar macrophages as a target cell. Labeled chrysotile fibers were found to retain >95% of their ability to stimulate these cells. The labeling procedure described in this study should be useful in preparing labeled fibers to investigate both in vitro and in vivo phenomena. (author).}
doi = {10.1016/0883-2889(91)90151-P}
journal = {Applied Radiation and Isotopes (International Journal of Radiation Applications and Instrumentation, Part A); (United Kingdom)}
volume = {42:6}
journal type = {AC}
place = {United Kingdom}
year = {1991}
month = {Jan}
}