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Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody

Journal Article:

Abstract

Rat monoclonal antibodies were prepared by immunising rats with human colorectal carcinoma cell membranes and fusing splenic lymphocytes with a rat myeloma. Hybridoma supernatants were screened by binding assays on membranes prepared from colorectal carcinoma tissue. One hybridoma supernatant, containing a monoclonal antibody with high binding activity on malignant compared to normal colon sections, was grown in large quantities in serum-free medium. After ammonium sulphate precipitation the antibody was purified by ion-exchange chromatography and labelled with /sup 131/I. Radiolabelled antibody was administered i.v. to 27 patients with colonic and other tumours. Scintigrams were obtained at 48 h. Computerised subtraction of the blood pool image revealed localised areas of uptake corresponding with areas of known disease in 13/16 patients with colorectal carcinoma and 3/4 patients with breast cancer.
Authors:
Smedley, H M; Ritson, A; Wraight, P; Sikora, K; [1]  Hinchingbrooke Hospital, Huntingdon (UK)); Finan, P; [2]  Lennox, E S; Takei, F [3] 
  1. Addenbrooke's Hospital, Cambridge (UK)
  2. St. James Hospital, Leeds (UK)
  3. Medical Research Council, Cambridge (UK)
Publication Date:
Feb 01, 1983
Product Type:
Journal Article
Reference Number:
AIX-14-795917; EDB-83-201092
Resource Relation:
Journal Name: Br. J. Cancer; (United Kingdom); Journal Volume: 47:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOMAS; DIAGNOSIS; INDIUM 131; DIAGNOSTIC USES; METASTASES; MONOCLONAL ANTIBODIES; LABELLING; BIOLOGICAL LOCALIZATION; SCINTISCANNING; ANTIBODIES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; COUNTING TECHNIQUES; DIAGNOSTIC TECHNIQUES; DISEASES; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOTOPES; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPE SCANNING; RADIOISOTOPES; SECONDS LIVING RADIOISOTOPES; USES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5505184
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BJCAA
Submitting Site:
HEDB
Size:
Pages: 253-259
Announcement Date:

Journal Article:

Citation Formats

Smedley, H M, Ritson, A, Wraight, P, Sikora, K, Hinchingbrooke Hospital, Huntingdon (UK)), Finan, P, Lennox, E S, and Takei, F. Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody. United Kingdom: N. p., 1983. Web.
Smedley, H M, Ritson, A, Wraight, P, Sikora, K, Hinchingbrooke Hospital, Huntingdon (UK)), Finan, P, Lennox, E S, & Takei, F. Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody. United Kingdom.
Smedley, H M, Ritson, A, Wraight, P, Sikora, K, Hinchingbrooke Hospital, Huntingdon (UK)), Finan, P, Lennox, E S, and Takei, F. 1983. "Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody." United Kingdom.
@misc{etde_5505184,
title = {Localisation of metastatic carcinoma by a radiolabelled monoclonal antibody}
author = {Smedley, H M, Ritson, A, Wraight, P, Sikora, K, Hinchingbrooke Hospital, Huntingdon (UK)), Finan, P, Lennox, E S, and Takei, F}
abstractNote = {Rat monoclonal antibodies were prepared by immunising rats with human colorectal carcinoma cell membranes and fusing splenic lymphocytes with a rat myeloma. Hybridoma supernatants were screened by binding assays on membranes prepared from colorectal carcinoma tissue. One hybridoma supernatant, containing a monoclonal antibody with high binding activity on malignant compared to normal colon sections, was grown in large quantities in serum-free medium. After ammonium sulphate precipitation the antibody was purified by ion-exchange chromatography and labelled with /sup 131/I. Radiolabelled antibody was administered i.v. to 27 patients with colonic and other tumours. Scintigrams were obtained at 48 h. Computerised subtraction of the blood pool image revealed localised areas of uptake corresponding with areas of known disease in 13/16 patients with colorectal carcinoma and 3/4 patients with breast cancer.}
journal = {Br. J. Cancer; (United Kingdom)}
volume = {47:2}
journal type = {AC}
place = {United Kingdom}
year = {1983}
month = {Feb}
}