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Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation

Journal Article:

Abstract

A rapid mixing system of the stopped-flow type, used with small-angle X-ray scattering equipment using synchrotron radiation, is described. The process of aggregation of bovine serum albumin was traced with a time interval of 50s, initiated upon cleavage of its disulfide bonds by reduction with dithiothreitol. The results indicate that a 218-fold molar excess of dithiothreitol over the number of moles of disulfide bonds in bovine serum albumin is sufficient to initiate the reaction immediately after mixing, which reaches equilibrium in about 15 min. On the other hand, half this amount is not sufficient to initiate the reaction, so that the reaction is delayed by about 150s. Such a single-shot time-resolved experiment showed that experiments with a time interval of 100 ms are possible with repeated multi-shot runs. 26 refs.; 8 figs.
Publication Date:
Nov 01, 1985
Product Type:
Journal Article
Reference Number:
AIX-17-047754; EDB-86-130160
Resource Relation:
Journal Name: Biophys. Chem.; (Netherlands); Journal Volume: 23:1-2
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; AGGLOMERATION; REDUCTION; X-RAY DIFFRACTOMETERS; SYNCHROTRON RADIATION; TIME RESOLUTION; DATA ACQUISITION SYSTEMS; DISULFIDES; DITHIOLS; REDUCING AGENTS; X-RAY DIFFRACTION; BREMSSTRAHLUNG; CHEMICAL REACTIONS; COHERENT SCATTERING; DIFFRACTION; DIFFRACTOMETERS; ELECTROMAGNETIC RADIATION; MEASURING INSTRUMENTS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PROTEINS; RADIATIONS; REAGENTS; RESOLUTION; SCATTERING; THIOLS; TIMING PROPERTIES; 440105* - Radiation Instrumentation- Radiometric Instruments- (-1987); 550200 - Biochemistry
OSTI ID:
5493840
Research Organizations:
Osaka City Univ., Japan. Faculty of Engineering; Kyoto Univ., Japan. Inst. for Chemical Research; Tohoku Univ., Sendai, Japan. Faculty of Science; National Lab. for High Energy Physics, Oho, Ibaraki, Japan; Hokkaido Univ., Sapporo, Japan. Faculty of Science
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BICIA
Submitting Site:
INIS
Size:
Pages: 115-124
Announcement Date:

Journal Article:

Citation Formats

Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio. Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation. Netherlands: N. p., 1985. Web.
Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, & Muroga, Yoshio. Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation. Netherlands.
Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio. 1985. "Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation." Netherlands.
@misc{etde_5493840,
title = {Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation}
author = {Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio}
abstractNote = {A rapid mixing system of the stopped-flow type, used with small-angle X-ray scattering equipment using synchrotron radiation, is described. The process of aggregation of bovine serum albumin was traced with a time interval of 50s, initiated upon cleavage of its disulfide bonds by reduction with dithiothreitol. The results indicate that a 218-fold molar excess of dithiothreitol over the number of moles of disulfide bonds in bovine serum albumin is sufficient to initiate the reaction immediately after mixing, which reaches equilibrium in about 15 min. On the other hand, half this amount is not sufficient to initiate the reaction, so that the reaction is delayed by about 150s. Such a single-shot time-resolved experiment showed that experiments with a time interval of 100 ms are possible with repeated multi-shot runs. 26 refs.; 8 figs.}
journal = {Biophys. Chem.; (Netherlands)}
volume = {23:1-2}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Nov}
}