Abstract
A rapid mixing system of the stopped-flow type, used with small-angle X-ray scattering equipment using synchrotron radiation, is described. The process of aggregation of bovine serum albumin was traced with a time interval of 50s, initiated upon cleavage of its disulfide bonds by reduction with dithiothreitol. The results indicate that a 218-fold molar excess of dithiothreitol over the number of moles of disulfide bonds in bovine serum albumin is sufficient to initiate the reaction immediately after mixing, which reaches equilibrium in about 15 min. On the other hand, half this amount is not sufficient to initiate the reaction, so that the reaction is delayed by about 150s. Such a single-shot time-resolved experiment showed that experiments with a time interval of 100 ms are possible with repeated multi-shot runs. 26 refs.; 8 figs.
Citation Formats
Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio.
Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation.
Netherlands: N. p.,
1985.
Web.
Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, & Muroga, Yoshio.
Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation.
Netherlands.
Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio.
1985.
"Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation."
Netherlands.
@misc{etde_5493840,
title = {Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation}
author = {Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio}
abstractNote = {A rapid mixing system of the stopped-flow type, used with small-angle X-ray scattering equipment using synchrotron radiation, is described. The process of aggregation of bovine serum albumin was traced with a time interval of 50s, initiated upon cleavage of its disulfide bonds by reduction with dithiothreitol. The results indicate that a 218-fold molar excess of dithiothreitol over the number of moles of disulfide bonds in bovine serum albumin is sufficient to initiate the reaction immediately after mixing, which reaches equilibrium in about 15 min. On the other hand, half this amount is not sufficient to initiate the reaction, so that the reaction is delayed by about 150s. Such a single-shot time-resolved experiment showed that experiments with a time interval of 100 ms are possible with repeated multi-shot runs. 26 refs.; 8 figs.}
journal = []
volume = {23:1-2}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Nov}
}
title = {Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle X-ray scattering technique with synchrotron radiation}
author = {Ueki, Tatzuo, Inoko, Yoji, Hiragi, Yuzuru, Kataoka, Mikio, Amemiya, Yoshiyuki, Izumi, Yoshinobu, Tagawa, Hiroyuki, and Muroga, Yoshio}
abstractNote = {A rapid mixing system of the stopped-flow type, used with small-angle X-ray scattering equipment using synchrotron radiation, is described. The process of aggregation of bovine serum albumin was traced with a time interval of 50s, initiated upon cleavage of its disulfide bonds by reduction with dithiothreitol. The results indicate that a 218-fold molar excess of dithiothreitol over the number of moles of disulfide bonds in bovine serum albumin is sufficient to initiate the reaction immediately after mixing, which reaches equilibrium in about 15 min. On the other hand, half this amount is not sufficient to initiate the reaction, so that the reaction is delayed by about 150s. Such a single-shot time-resolved experiment showed that experiments with a time interval of 100 ms are possible with repeated multi-shot runs. 26 refs.; 8 figs.}
journal = []
volume = {23:1-2}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Nov}
}