Abstract
The use of genetic engineering methodologies in breeding programmes seems to be very promising to find new resistance-related genes present in other phyla, to clone and transfer them into plants; and, to shorten the time to obtain an improved genotype since only a single gene is involved in this process. The main ``bottle-neck`` to apply this scheme in chickpea and cowpea is the absence of a reliable protocol of regeneration and genetic transformation. In this frame, following some pilot experiments on these grain legumes to induce regeneration and gene transfer, we attempted to find a regeneration medium, assay the effect of different hormones on young tissues; and, to select the best procedures for transfer of genes into the plant genome.
Filippone, E;
Monti, L
[1]
- Department of Agronomy and Plant Genetics, Univ. of Naples Federico 2, Naples (Italy)
Citation Formats
Filippone, E, and Monti, L.
Chickpea and cowpea grain improvement using mutation and other advanced genetic techniques.
IAEA: N. p.,
1997.
Web.
Filippone, E, & Monti, L.
Chickpea and cowpea grain improvement using mutation and other advanced genetic techniques.
IAEA.
Filippone, E, and Monti, L.
1997.
"Chickpea and cowpea grain improvement using mutation and other advanced genetic techniques."
IAEA.
@misc{etde_548104,
title = {Chickpea and cowpea grain improvement using mutation and other advanced genetic techniques}
author = {Filippone, E, and Monti, L}
abstractNote = {The use of genetic engineering methodologies in breeding programmes seems to be very promising to find new resistance-related genes present in other phyla, to clone and transfer them into plants; and, to shorten the time to obtain an improved genotype since only a single gene is involved in this process. The main ``bottle-neck`` to apply this scheme in chickpea and cowpea is the absence of a reliable protocol of regeneration and genetic transformation. In this frame, following some pilot experiments on these grain legumes to induce regeneration and gene transfer, we attempted to find a regeneration medium, assay the effect of different hormones on young tissues; and, to select the best procedures for transfer of genes into the plant genome.}
place = {IAEA}
year = {1997}
month = {Dec}
}
title = {Chickpea and cowpea grain improvement using mutation and other advanced genetic techniques}
author = {Filippone, E, and Monti, L}
abstractNote = {The use of genetic engineering methodologies in breeding programmes seems to be very promising to find new resistance-related genes present in other phyla, to clone and transfer them into plants; and, to shorten the time to obtain an improved genotype since only a single gene is involved in this process. The main ``bottle-neck`` to apply this scheme in chickpea and cowpea is the absence of a reliable protocol of regeneration and genetic transformation. In this frame, following some pilot experiments on these grain legumes to induce regeneration and gene transfer, we attempted to find a regeneration medium, assay the effect of different hormones on young tissues; and, to select the best procedures for transfer of genes into the plant genome.}
place = {IAEA}
year = {1997}
month = {Dec}
}