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Psoralen plus ultraviolet radiation-induced inhibition of DNA synthesis and viability in human lymphoid cells in vitro

Journal Article:

Abstract

The present study investigated whether conditions of 8-methoxypsoralen (8-MOP) concentration and of exposure to high intensity long wavelength ultraviolet radiation (UV-A) during psoriasis and mycosis fungoides therapy might be sufficient to result directly in decreased lymphoid cell DNA synthesis and viability in vitro. Tritiated thymidine (/sup 3/HtdR) incorporation and cell growth following UV-A exposure alone or with 8-MOP was examined in peripheral blood lymphocytes and in Ebstein-Barr virus transformed human lymphoblastoid cell lines. UV-A exposure alone induced a dose-dependent inhibition of /sup 3/HTdR incorporation in both types of lymphoid cells. Pre-incubation with 0.1 ..mu..g/ml 8-MOP before UV-A exposure induced a significantly greater inhibition of /sup 3/HTdr incorporation. Further inhibition of /sup 3/HTdR incorporation was observed by preincubation of the lymphoblastoid cells with 1.0 ..mu..g/ml 8-MOP but not in the lymphocytes. The concentration of viable lymphoblastoid cells did not decrease below the original concentration after the highest dose of UV-A alone (29,00 J/m/sup 2/) but preincubation with 0.1 ..mu..g/ml 8-MOP resulted in 40% and 0.6% survival respectively after 3000 J/m/sup 2/. This study suggested that the low doses of 8-MOP and UV-A received by patients' lymphocytes may be sufficient to explain the decreased DNA synthesis found in their circulating leucocytes. (author).
Authors:
Kraemer, K H; Waters, H L; [1]  Ellingson, O L; Tarone, R E
  1. National Cancer Inst., Bethesda, MD (USA)
Publication Date:
Aug 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-505048; EDB-80-072957
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 30:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA REPLICATION; DOSE-RESPONSE RELATIONSHIPS; NEAR ULTRAVIOLET RADIATION; BIOLOGICAL RADIATION EFFECTS; PSORALEN; BIOLOGICAL EFFECTS; RADIOTHERAPY; SIDE EFFECTS; BIOSYNTHESIS; EXTERNAL IRRADIATION; GROWTH; IN VITRO; LABELLED COMPOUNDS; LYMPHOCYTES; MAN; SURVIVAL CURVES; THYMIDINE; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; ANTICOAGULANTS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DRUGS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; IRRADIATION; LEUKOCYTES; MAMMALS; MATERIALS; MEDICINE; NUCLEAR MEDICINE; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PRIMATES; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADIOLOGY; RIBOSIDES; SOMATIC CELLS; SYNTHESIS; THERAPY; ULTRAVIOLET RADIATION; VERTEBRATES; 550603* - Medicine- External Radiation in Therapy- (1980-); 560301 - Chemicals Metabolism & Toxicology- Cells- (-1987); 560121 - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
5451893
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 263-270
Announcement Date:

Journal Article:

Citation Formats

Kraemer, K H, Waters, H L, Ellingson, O L, and Tarone, R E. Psoralen plus ultraviolet radiation-induced inhibition of DNA synthesis and viability in human lymphoid cells in vitro. United Kingdom: N. p., 1979. Web.
Kraemer, K H, Waters, H L, Ellingson, O L, & Tarone, R E. Psoralen plus ultraviolet radiation-induced inhibition of DNA synthesis and viability in human lymphoid cells in vitro. United Kingdom.
Kraemer, K H, Waters, H L, Ellingson, O L, and Tarone, R E. 1979. "Psoralen plus ultraviolet radiation-induced inhibition of DNA synthesis and viability in human lymphoid cells in vitro." United Kingdom.
@misc{etde_5451893,
title = {Psoralen plus ultraviolet radiation-induced inhibition of DNA synthesis and viability in human lymphoid cells in vitro}
author = {Kraemer, K H, Waters, H L, Ellingson, O L, and Tarone, R E}
abstractNote = {The present study investigated whether conditions of 8-methoxypsoralen (8-MOP) concentration and of exposure to high intensity long wavelength ultraviolet radiation (UV-A) during psoriasis and mycosis fungoides therapy might be sufficient to result directly in decreased lymphoid cell DNA synthesis and viability in vitro. Tritiated thymidine (/sup 3/HtdR) incorporation and cell growth following UV-A exposure alone or with 8-MOP was examined in peripheral blood lymphocytes and in Ebstein-Barr virus transformed human lymphoblastoid cell lines. UV-A exposure alone induced a dose-dependent inhibition of /sup 3/HTdR incorporation in both types of lymphoid cells. Pre-incubation with 0.1 ..mu..g/ml 8-MOP before UV-A exposure induced a significantly greater inhibition of /sup 3/HTdr incorporation. Further inhibition of /sup 3/HTdR incorporation was observed by preincubation of the lymphoblastoid cells with 1.0 ..mu..g/ml 8-MOP but not in the lymphocytes. The concentration of viable lymphoblastoid cells did not decrease below the original concentration after the highest dose of UV-A alone (29,00 J/m/sup 2/) but preincubation with 0.1 ..mu..g/ml 8-MOP resulted in 40% and 0.6% survival respectively after 3000 J/m/sup 2/. This study suggested that the low doses of 8-MOP and UV-A received by patients' lymphocytes may be sufficient to explain the decreased DNA synthesis found in their circulating leucocytes. (author).}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {30:2}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Aug}
}