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/sup 3/H-dextran method for measurements of the blood volume in the rat choroid

Journal Article:

Abstract

A new method was developed using /sup 3/H-dextran for measuring the blood volume in the choroid. Under pentobarbital-anesthesia, albino rats weighing 200 grams were perfused through the left ventricle with a 2.5 percent glutaraldehyde solution containing the radioactive dextran. The procedure allowed exchange of the choroidal blood with the /sup 3/H-dextran solution with a simultaneous fixation of the choroid. The blood volume in the choroid was calculated from the radioactivity count, which is estimated to be 1.690 x 10/sup -4/ ml per mg wet weight and 5.070 x 10/sup -4/ ml per mg dry weight. Epinephrine subconjunctivally injected diminished the blood volume in the choroid by 68 percent. Pretreatment with lidocaine almost nullified the effect of epinephrine. Applicability of this method to the analytical study of the choroidal circulation is discussed.
Authors:
Matsusaka, T; [1]  Morimoto, K; Kikkawa, Y
  1. Osaka Prefectural Center for Adult Diseases (Japan)
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-13-664629; EDB-82-090610
Resource Relation:
Journal Name: Jpn. J. Ophthalmol.; (Japan); Journal Volume: 24:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; BLOOD FLOW; TRACER TECHNIQUES; ADRENALINE; BLOOD CIRCULATION; DEXTRAN; LABELLED COMPOUNDS; RATS; RESPONSE MODIFYING FACTORS; TRITIUM COMPOUNDS; UVEA; ADRENAL HORMONES; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BLOOD SUBSTITUTES; BODY; BODY AREAS; CARBOHYDRATES; CARDIOTONICS; CARDIOVASCULAR AGENTS; DRUGS; EYES; FACE; HEAD; HEMATOLOGIC AGENTS; HORMONES; ISOTOPE APPLICATIONS; MAMMALS; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANS; POLYSACCHARIDES; RODENTS; SACCHARIDES; SENSE ORGANS; STEROID HORMONES; SYMPATHOMIMETICS; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
5431200
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JJOPA
Submitting Site:
HEDB
Size:
Pages: 439-443
Announcement Date:

Journal Article:

Citation Formats

Matsusaka, T, Morimoto, K, and Kikkawa, Y. /sup 3/H-dextran method for measurements of the blood volume in the rat choroid. Japan: N. p., 1980. Web.
Matsusaka, T, Morimoto, K, & Kikkawa, Y. /sup 3/H-dextran method for measurements of the blood volume in the rat choroid. Japan.
Matsusaka, T, Morimoto, K, and Kikkawa, Y. 1980. "/sup 3/H-dextran method for measurements of the blood volume in the rat choroid." Japan.
@misc{etde_5431200,
title = {/sup 3/H-dextran method for measurements of the blood volume in the rat choroid}
author = {Matsusaka, T, Morimoto, K, and Kikkawa, Y}
abstractNote = {A new method was developed using /sup 3/H-dextran for measuring the blood volume in the choroid. Under pentobarbital-anesthesia, albino rats weighing 200 grams were perfused through the left ventricle with a 2.5 percent glutaraldehyde solution containing the radioactive dextran. The procedure allowed exchange of the choroidal blood with the /sup 3/H-dextran solution with a simultaneous fixation of the choroid. The blood volume in the choroid was calculated from the radioactivity count, which is estimated to be 1.690 x 10/sup -4/ ml per mg wet weight and 5.070 x 10/sup -4/ ml per mg dry weight. Epinephrine subconjunctivally injected diminished the blood volume in the choroid by 68 percent. Pretreatment with lidocaine almost nullified the effect of epinephrine. Applicability of this method to the analytical study of the choroidal circulation is discussed.}
journal = {Jpn. J. Ophthalmol.; (Japan)}
volume = {24:4}
journal type = {AC}
place = {Japan}
year = {1980}
month = {Jan}
}