Abstract
A method is described for determination of glucose, sucrose, and lactose in biological solutions using a glucose enzyme electrode characterized by high sensitivity and selectivity. The enzyme membrane (15 nm thick) is prepared from glucose oxidase isolated from Penicillium vitale. Glucose is determined in one minute (using static currents) or in 12 s (using registered current in a kinetic regime). Phosphate buffer (5-10 mM) is the only reagent required for analysis. Determination of sucrose and lactose require prior hydrolysis with 17.8% HCl at 70 degrees Celcius for O.5 and lO.7 minutes, respectively.
Citation Formats
Kulys, J, and Pesliakiene, M.
Analytical systems as a basis for immobilized enzymes. 3. Use of a glucose enzyme electrode to determine carbohydrates in biological solutions.
USSR: N. p.,
1981.
Web.
Kulys, J, & Pesliakiene, M.
Analytical systems as a basis for immobilized enzymes. 3. Use of a glucose enzyme electrode to determine carbohydrates in biological solutions.
USSR.
Kulys, J, and Pesliakiene, M.
1981.
"Analytical systems as a basis for immobilized enzymes. 3. Use of a glucose enzyme electrode to determine carbohydrates in biological solutions."
USSR.
@misc{etde_5428535,
title = {Analytical systems as a basis for immobilized enzymes. 3. Use of a glucose enzyme electrode to determine carbohydrates in biological solutions}
author = {Kulys, J, and Pesliakiene, M}
abstractNote = {A method is described for determination of glucose, sucrose, and lactose in biological solutions using a glucose enzyme electrode characterized by high sensitivity and selectivity. The enzyme membrane (15 nm thick) is prepared from glucose oxidase isolated from Penicillium vitale. Glucose is determined in one minute (using static currents) or in 12 s (using registered current in a kinetic regime). Phosphate buffer (5-10 mM) is the only reagent required for analysis. Determination of sucrose and lactose require prior hydrolysis with 17.8% HCl at 70 degrees Celcius for O.5 and lO.7 minutes, respectively.}
journal = []
volume = {3}
journal type = {AC}
place = {USSR}
year = {1981}
month = {Jan}
}
title = {Analytical systems as a basis for immobilized enzymes. 3. Use of a glucose enzyme electrode to determine carbohydrates in biological solutions}
author = {Kulys, J, and Pesliakiene, M}
abstractNote = {A method is described for determination of glucose, sucrose, and lactose in biological solutions using a glucose enzyme electrode characterized by high sensitivity and selectivity. The enzyme membrane (15 nm thick) is prepared from glucose oxidase isolated from Penicillium vitale. Glucose is determined in one minute (using static currents) or in 12 s (using registered current in a kinetic regime). Phosphate buffer (5-10 mM) is the only reagent required for analysis. Determination of sucrose and lactose require prior hydrolysis with 17.8% HCl at 70 degrees Celcius for O.5 and lO.7 minutes, respectively.}
journal = []
volume = {3}
journal type = {AC}
place = {USSR}
year = {1981}
month = {Jan}
}